샐러드와 새싹채소에서 Enterococcus의 분리와 항생제 내성특성
Antibiotic Resistances of Enterococcus Isolated from Salad and Sprout.
0
조회
0
다운로드
10
인용
0
참고문헌
초록
To evaluate the antibiotic resistance of Enterococcus from salad and sprout, Enterococcus were isolated and identified from 47 salad samples and 37 sprout samples, and then their antibiotic resistances were analyzed. Ninety five Enterococcus, 41 strains from salad and 54 strains from sprout, were ultimately isolated. The frequent Enterococcus in salad and sprout were E. gallinarum, E. faecalis, E. faecium, E. hirae, and E. avium. Minimum inhibitory concentrations of the isolates for vancomycin were below 4 μg/mL, which were not high levels of resistance. All Enterococcus proved to be resistant to streptomycin and chloramphenicol. Twenty two percentage of the isolates were resistant to penicillin, however, almost the isolates were sensitive to tetracycline. Eighteen percentage of the isolates were resistant to erythromycin. All E. faecium and E.faecalis were found to be ampicillin-resistant, and seven E. faecalis and five E. faecium were resistant to rifampicin. Overall antibiotic resistances of Enterococcus isolates were relatively low and low resistance to vancomycin was similar to those evidenced by Enterococcus isolated from the other foods. Therefore, there may be no special risk from the antibiotics resistances of Enterococcus and especially vancomycin-resistant Enterococcus from the fresh-cut salads and the sprouts.
Abstract
To evaluate the antibiotic resistance of Enterococcus from salad and sprout, Enterococcus were isolated and identified from 47 salad samples and 37 sprout samples, and then their antibiotic resistances were analyzed. Ninety five Enterococcus, 41 strains from salad and 54 strains from sprout, were ultimately isolated. The frequent Enterococcus in salad and sprout were E. gallinarum, E. faecalis, E. faecium, E. hirae, and E. avium. Minimum inhibitory concentrations of the isolates for vancomycin were below 4 μg/mL, which were not high levels of resistance. All Enterococcus proved to be resistant to streptomycin and chloramphenicol. Twenty two percentage of the isolates were resistant to penicillin, however, almost the isolates were sensitive to tetracycline. Eighteen percentage of the isolates were resistant to erythromycin. All E. faecium and E.faecalis were found to be ampicillin-resistant, and seven E. faecalis and five E. faecium were resistant to rifampicin. Overall antibiotic resistances of Enterococcus isolates were relatively low and low resistance to vancomycin was similar to those evidenced by Enterococcus isolated from the other foods. Therefore, there may be no special risk from the antibiotics resistances of Enterococcus and especially vancomycin-resistant Enterococcus from the fresh-cut salads and the sprouts.
참고문헌 (21)
- Ahn, Y. S., "Antimicrobial effects of organic acids and ethanol on several foodborne microorganism", Korean J. Food Sci. Technol, 31, pp.1315~1323 (1999)
- Bates, J., "Farm animals as a putative reservoir for vancomycin-resistant enterococci infection in man", J. Antimicrob. Chemoth, 34, pp.507~516 (1994)
- Chadwick, P. R., "Glycopeptideresistant enterococci isolated from uncooked meat", J. Antimicrob. Chemoth, 38, pp.908~909 (1996)
- Cho, Y. S., "Comparison of antimicrobial susceptibility of vancomycin resistant enterococci from animal and human", Korean J. Vet. Public Health, 27, pp.17~29 (2003)
- Collins, M. D., "E.avium nom. rev.,comb. nov.; E. casseliflavus nom. rev., comb. nov. E.durans nom. rev., combnov.; E. gallinarum comb nov", Int. Syst. Bacteriol, 34, pp.220~223 (1984)
- Devriese, L. A., "Phenotypic identification of the genus Enterococcus and differentiation of phylogentically distanct enterococcal species and species group", J. Appl. Becteriol, 75, pp.339~408 (1993)
- Fackalm, R. R., "Identification of Enterococcus species isolated from human infections by a conventional test scheme", J. Clin. Microbiol, 27, pp.731~734 (1989)
- Johnson, A. P., "Resistance to vancomycin and teicoplanin: An emerging clinical problem", Clin. Microbiol. Rev, 3, pp.280~291 (1990)
- Kim, J. K., "A review study of food poisoning in Korea", J. Food Hyg. Saf, 4, pp.199~255 (1989)
- Klare, I., "vanA-Mediated high-level glycopeptide resistance in Enterococcus faecium from animal husbandry", FEMS Microbiol. Lett, 125, pp.165~172 (1995)
- Klein, G., "Antibiotic resistance patterns of enterococci and occurrence of vancomycinresistant enterococci in raw minced beef and pork in Germany", Appl. Environ. Microbiol, 64, pp.1825~1830 (1998)
- Mandel, G.L., "Streptococcus bovis, and Leuconostoc. spp., A Principles and Practice of Infectious Disease", pp.1826~1835 (1995), Churchill Living Stone
- Moellering, R.C., "Vancomycin-resistant enterococci", Clin. Infect. Dis, 26, pp.1196~1199 (1998)
- Mundt, O.J., "Enterococci, rgey`s Manual of Systemic Bacteriology. Vol. 2", pp.1063~1065 (1986), Williams and Wilkins
- Murray, B.E., "life and times of the Entrococcus", Clin. Microbiol. Rev, 3, pp.46~65 (1990)
- "NCCLS document M2-A7, erformance standards for antimicrobial disk susceptibility tests; Approved standard M2-A7" (2000), National Committee for Clinical Laboratory Standard
- Noble, W.C., "Co-transfer of vancomycin and other resistance genes from Enterococcus faecalis NCTC 12201 to Staphylococcus aureus", FEMS Microbiol. Lett, 93, pp.195~198 (1992)
- Pavia, M., "Vancomycin resistance and antibiotic susceptibility of enterococci in raw meat", J. Food Protect, 7, pp.912~915 (2000)
- Schlefer, K.H., "Molecular and chemotaxonomic approach to the classification of streptococci, enterococci and lactococci", Review. Syst. Appl. Microbiol, 10, pp.1~18 (1987)
- Seo, K.S., "Development of multiplex PCR for detection of vancomycin resistant enterococci(VRE) and epidemiological application in Korea", Korean J. Vet. Res, 39, pp.343~352 (1999)
- Zarazaga, M., "In vitro activities of ketolide MHR 3647, mactolides, and other antibiotics against Lacobacillus, Leuconostoc, and Pediococcus isolates", Antimicrob. Agents Ch, 43, pp.3039~3041 (1999)