Clinical outcomes of vitrified-thawed embryo transfer using a pull and cut straw method
2
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
Objective: To compare the clinical outcomes of patients with vitrified-thawed embryos transferred using either the 0.25 mL straw method and the pull and cut straw (PNC) method. To evaluate the clinical outcomes of patients with transferred embryos that underwent assisted hatching at the cleaved embryo (day 3) or the blastocyst (day 5) stage. Methods: The study population consisted of women who underwent vitrified-warmed embryo transfer between May 2000 and December 2011 and assisted hatching was performed after warming of embryos. Cycles of thawing between assisted hatching treated and non treated groups were compared for survival and pregnancy rates. Results: The PNC vitrification method improved survival and pregnancy rates in partial lysed embryos. While assisted hatching did not affect the developmental and clinical pregnancy rates of the vitrified-warmed blastocyst group, it did increase the pregnancy rate of poor quality vitrified-warmed cleaved embryos. Conclusion: These results suggest that PNC may increase the number of clinical pregnancies via the vitrification of both cleaved embryos and blastocysts. In addition, selective assisted hatching treatment of embryos that show a poor prognosis after warming may increase the rate of clinical pregnancy.
Abstract
Objective: To compare the clinical outcomes of patients with vitrified-thawed embryos transferred using either the 0.25 mL straw method and the pull and cut straw (PNC) method. To evaluate the clinical outcomes of patients with transferred embryos that underwent assisted hatching at the cleaved embryo (day 3) or the blastocyst (day 5) stage. Methods: The study population consisted of women who underwent vitrified-warmed embryo transfer between May 2000 and December 2011 and assisted hatching was performed after warming of embryos. Cycles of thawing between assisted hatching treated and non treated groups were compared for survival and pregnancy rates. Results: The PNC vitrification method improved survival and pregnancy rates in partial lysed embryos. While assisted hatching did not affect the developmental and clinical pregnancy rates of the vitrified-warmed blastocyst group, it did increase the pregnancy rate of poor quality vitrified-warmed cleaved embryos. Conclusion: These results suggest that PNC may increase the number of clinical pregnancies via the vitrification of both cleaved embryos and blastocysts. In addition, selective assisted hatching treatment of embryos that show a poor prognosis after warming may increase the rate of clinical pregnancy.
참고문헌 (24)
- Rall WF, "Ice-free cryopreservation of mouse embryos at -196 degrees C by vitrification", Nature, 313, pp.573~575 (1985)
- Desai N, "Cryoloop vitrification of human day 3 cleavage-stage embryos: post-vitrification development, pregnancy outcomes and live births", Reprod Biomed Online, 14, pp.208~213 (2007)
- 김현정, "동결 해동한 포배 이식에 있어서 동결시기가 임신결과에 미치는 영향", Clinical and Experimental Reproductive Medicine, 37(1), pp.57~64 (2010), 대한생식의학회
- Mi Ra Shin, "In vitro development and gene expression of frozen-thawed 8-cell stage mouse embryos following slow freezing or vitrification", Clinical and Experimental Reproductive Medicine, 38(4), pp.203~209 (2011), 대한생식의학회
- Drobnis EZ, "Biophysical properties of the zona pellucida measured by capillary suction: is zona hardening a mechanical phenomenon?", J Exp Zool, 245, pp.206~219 (1988)
- Cohen J, "Implantation enhancement by selective assisted hatching using zona drilling of human embryos with poor prognosis", Hum Reprod, 7, pp.685~691 (1992)
- De Vos A, "Zona hardening, zona drilling and assisted hatching: new achievements in assisted reproduction", Cells Tissues Organs, 166, pp.220~227 (2000)
- El-Toukhy T, "Effect of blastomere loss on the outcome of frozen embryo replacement cycles", Fertil Steril, 79, pp.1106~1111 (2003)
- Tucker MJ, "Partial dissection of the zona pellucida of frozen-thawed human embryos may enhance blastocyst hatching, implantation, and pregnancy rates", Am J Obstet Gynecol, 165, pp.341~344 (1991)
- Check JH, "The effect of assisted hatching on pregnancy rates after frozen embryo transfer", Fertil Steril, 65, pp.254~257 (1996)
- Nagy ZP, "Removal of lysed blastomeres from frozen-thawed embryos improves implantation and pregnancy rates in frozen embryo transfer cycles", Fertil Steril, 84, pp.1606~1612 (2005)
- Martins WP, "Assisted hatching of human embryos : a systematic review and meta-analysis of randomized controlled trials", Hum Reprod Update, 17, pp.438~453 (2011)
- Schiewe MC, "Physiological characterization of blastocyst hatching mechanisms by use of a mouse antihatching model", Fertil Steril, 63, pp.288~294 (1995)
- Lanzendorf SE, "A prospective, randomized, double-blind study for the evaluation of assisted hatching in patients with advanced maternal age", Hum Reprod, 13, pp.409~413 (1998)
- Vanderzwalmen P, "Vitrification of human blastocysts with the Hemi-Straw carrier: application of assisted hatching after thawing", Hum Reprod, 18, pp.1504~1511 (2003)
- Kuwayama M, "Highly efficient vitrification method for cryopreservation of human oocytes", Reprod Biomed Online, 11, pp.300~308 (2005)
- Mukaida T, "Vitrification of human blastocysts using cryoloops : clinical outcome of 223 cycles", Hum Reprod, 18, pp.384~391 (2003)
- Huisman GJ, "Implantation rates after in vitro fertilization and transfer of a maximum of two embryos that have undergone three to five days of culture", Fertil Steril, 73, pp.117~122 (2000)
- Carroll J, "Freeze-thaw-induced changes of the zona pellucida explains decreased rates of fertilization in frozen-thawed mouse oocytes", J Reprod Fertil, 90, pp.547~553 (1990)
- Liu HC, "Assisted hatching facilitates earlier implantation", Fertil Steril, 60, pp.871~875 (1993)
- Malter HE, "Blastocyst formation and hatching in vitro following zona drilling of mouse and human embryos", Gamete Res, 24, pp.67~80 (1989)
- Hershlag A, "Effect of prefreeze assisted hatching on postthaw survival of mouse embryos", Fertil Steril, 84, pp.1752~1754 (2005)
- Cohen J, "Videocinematography of fresh and cryopreserved embryos : a retrospective analysis of embryonic morphology and implantation", Fertil Steril, 51, pp.820~827 (1989)
- Cohen J, "Assisted hatching of human embryos", J In Vitro Fert Embryo Transf, 8, pp.179~190 (1991)