Long Cut Straw Provides Stable the Rates of Survival, Pregnancy and Live Birth for Vitrification of Human Blasotcysts
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Most of the commercial devices for vitrification are directly immersed into the warming solution (WS) for increasing of warming rate. However, the previous modified cut standard straw (MCS) which has reported is difficult to immerse into the WS. The aim of this study was to investigate whether the long cut straw (LCS) could be useful as a stable tool for vitrified-warmed human blastocysts. A total of 138 vitrified-warmed cycles were performed between November 2013 and November 2014 (exclusion criteria: women ≥38 years old, poor responder, surgical retrieval sperm, and severe male factor). The artificial shrinkage was conducted using 29-gauge needles. Ethylene glycol and dimethyl sulfoxide (7.5% and 15% (v/v)) were used as cryoprotectants. Freezing and warming were conducted using the LCS tool. The cap of LCS was removed using the forceps in the liquid nitrogen (LN2) and then directly immersed into the first WS for 1 min at 37℃ (1 M sucrose). Only re-expanded blastocysts were transferred after it was cultured in sequential media for 18-20 h. A total of 294 blastocysts were warmed, and all were recovered (100%). Two hundred eighty-five embryos were survived (96.9%). The vitrifiedwarmed blastocysts of all patients were transferred without any cancellation. We were able to achieve a reasonable implantation (24.2%), following by clinical pregnancy (36.2%), which then continued to ongoing pregnancy (36.2%), and live birth (31.2%). Using LCS is achieved the acceptable rates of survival, pregnancy and live birth. Therefore, the LCS could be considered as a stable and simple tool for human embryo vitrificaton.
Abstract
Most of the commercial devices for vitrification are directly immersed into the warming solution (WS) for increasing of warming rate. However, the previous modified cut standard straw (MCS) which has reported is difficult to immerse into the WS. The aim of this study was to investigate whether the long cut straw (LCS) could be useful as a stable tool for vitrified-warmed human blastocysts. A total of 138 vitrified-warmed cycles were performed between November 2013 and November 2014 (exclusion criteria: women ≥38 years old, poor responder, surgical retrieval sperm, and severe male factor). The artificial shrinkage was conducted using 29-gauge needles. Ethylene glycol and dimethyl sulfoxide (7.5% and 15% (v/v)) were used as cryoprotectants. Freezing and warming were conducted using the LCS tool. The cap of LCS was removed using the forceps in the liquid nitrogen (LN2) and then directly immersed into the first WS for 1 min at 37℃ (1 M sucrose). Only re-expanded blastocysts were transferred after it was cultured in sequential media for 18-20 h. A total of 294 blastocysts were warmed, and all were recovered (100%). Two hundred eighty-five embryos were survived (96.9%). The vitrifiedwarmed blastocysts of all patients were transferred without any cancellation. We were able to achieve a reasonable implantation (24.2%), following by clinical pregnancy (36.2%), which then continued to ongoing pregnancy (36.2%), and live birth (31.2%). Using LCS is achieved the acceptable rates of survival, pregnancy and live birth. Therefore, the LCS could be considered as a stable and simple tool for human embryo vitrificaton.
참고문헌 (22)
- Bielanski A, "Microbial contamination of embryos and semen during long term banking in liquid nitrogen", Cryobiology, 46, pp.146~152 (2003)
- Bielanski A, "Viral contamination of embryos cryopreserved in liquid nitrogen", Cryobiology, 40, pp.110~116 (2000)
- Bielanski A, "Risk of contamination of germplasm during cryopreservation and cryobanking in IVF units", Hum Reprod, 24, pp.2457~2467 (2009)
- Cobo A, "Comparison of concomitant outcome achieved with fresh and cryopreserved donor oocytes vitrified by the Cryotop method", Fertil Steril, 89, pp.1657~1664 (2008)
- De Munck N, "Open versus closed oocyte vitrification in an oocyte donation programme: A prospective randomized sibling oocyte study", Hum Reprod, 31, pp.377~384 (2016)
- Gardner DK, "Blastocyst score affects implantation and pregnancy outcome: towards a single blastocyst transfer", Fertil Steril, 73, pp.1155~1158 (2000)
- Gvakharia M, "Implementation of an inexpensive method of vitrification and warming of human cleavage-stage embryos using cut standard straws", Fertil Steril, 95, pp.2552~2553 (2011)
- 정윤진, "Improvement of the Vitrification Method Suppressing the Disturbance of Meiotic Spindle and Chromosome Systems in Mature Oocytes", 발생과 생식, 18(2), pp.117~125 (2014), 한국발생생물학회
- 김현정, "동결 해동한 포배 이식에 있어서 동결시기가 임신결과에 미치는 영향", Clinical and Experimental Reproductive Medicine, 37(1), pp.57~64 (2010), 대한생식의학회
- Kuwayama M, "Comparison of open and closed methods for vitrification of human embryos and the elimination of potential contamination", Reprod Biomed Online, 11, pp.608~614 (2005)
- Kuwayama M, "Highly efficient vitrification method for cryopreservation of human oocytes", Reprod Biomed Online, 11, pp.300~308 (2005)
- 임준교, "Clinical outcomes of vitrified-thawed embryo transfer using a pull and cut straw method", Obstetrics & Gynecology Science, 56(03), pp.182~189 (2013), 대한산부인과학회[DOI]
- Loutradi KE, "Cryopreservation of human embryos by vitrification or slow freezing: A systematic review and meta-analysis", Fertil Steril, 90, pp.186~193 (2008)
- Marco-Jiménez F, "Development of cheaper embryo vitrifycation device using the minimum volume method", PLOS one, 11, pp.1~9 (2016)
- Mukaida T, "Artificial shrinkage of blastocoeles using either a micro-needle or a laser pulse prior to the cooling steps of vitrification improves survival rate and pregnancy outcome of vitrified human blastocysts", Hum Reprod, 21, pp.3246~3252 (2006)
- Rall WF, "Ice-free cryopreservation of mouse embryos at −196°C by vitrification", Nature, 313, pp.573~575 (1985)
- Mi Ra Shin, "In vitro development and gene expression of frozen-thawed 8-cell stage mouse embryos following slow freezing or vitrification", Clinical and Experimental Reproductive Medicine, 38(4), pp.203~209 (2011), 대한생식의학회
- Vajta G, "Improving cryopreservation systems", Theriogenology, 65, pp.236~244 (2006)
- Vajta G, "Are programmable freezers still needed in the embryo laboratory? Review on vitrification", Reprod Biomed Online, 12, pp.779~796 (2006)
- Vajta G, "Open versus closed systems for vitrification of human oocytes and embryos", Reprod Biomed Online, 30, pp.325~333 (2015)
- Vanderzwalmen P, "Closed carrier device: A reality to vitrify oocytes and embryos in aseptic conditions", Gynecol Obstet Fertil, 38, pp.541~546 (2010)
- Youssry M, "Current aspects of blastocyst cryopreservation", Reprod Biomed Online, 16, pp.311~320 (2008)