Hexane 분해 혼합균의 특성
Properties of a Hexane-Degrading Consortium.
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Hexane을 유일 탄소원으로 첨가한 무기염 배지에서 hexane 생분해 속도와 비성장속도를 구하였고, 14C-hexane을 이용하여 무기화(mineralization) 정도를 측정하였다. 또한, 16S rDNA PCR-DGGE 분석기법을 활용하여 consortium의 미생물 군집 특성을 조사하였다. Consortium CH의 최대 비성장속도 (max)값은 0.2 h-1이고, 최대 hexane 분해속도 (Vmax)와 포화상수 (Ks)는 각각 460mol·g-DCW-1·h-1 및 25.87mM 이었다. Consortium CH는 14C-hexane의 약 49.1%를 무기화하였고, 43.6%의 14C-hexane는 biomass를 증가시키는데 사용하였다. DGGE band로부터 얻은 clone들은 유류, biphenyl, PCE 및 폐가스 등과 같은 오염물질 분해능을 가진 세균들과 유사성이 가장 높았다. 본 연구에서 얻은 hexane 생분해용 consortium은 향후 hexane 제거용 생물학적 시스템을 개발하는데 유용하게 활용될 수 있다.감사의 글
Abstract
It was characterized the hexane biodegradation and mineralization using a hexane-degrading consortium, and analyzed its bacterial community structure by 16S rDNA PCR-DGGE (denaturing gradient gel electrophoresis). The specific growth rate (mmax) of the hexane-degrading consortium was 0.2 h-1 in mineral salt medium supplemented with hexane as a sole carbon source. The maximum degradation rate (Vmax) and saturation constant (Ks) of hexane of the consortium are 460 mmol·g-DCW-1·h-1 and 25.87 mM, respectively. In addition, this consortium could mineralize 49.1% of 14C-hexane to 14CO2, and 43.6% of 14C-hexane) was used for the growth of biomass. The clones isolated from the DGGE bands were closely related to the bacteria which were capable of degrading pollutants such as oil, biphenyl, PCE, and waste gases. The hexane-degrading consortium obtained in this study can be applied for the biological treatment of hexane.
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