토양 내 TPH(Total Petroleum Hydrocarbons)의 생물학적 분해 연구
In-situ Bioremediation of Total Petroleum Hydrocarbons-Contaminated Soil by Pseudomonas Species
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초록
본 연구실에서 확보한 diesel 분해 고효율 균주 Pseudomonas putida KDi 19, kerosene 분해 고효율 균주 P. aeruginosa K14, gasoline 분해 고효율 균주 P. putida G8,BTEX 분해 고효율 균주 P. putida BJ10, P. putida E41의5개의 고효율 균주를 컬럼 및 반응기에 적용하여 TPH의 생물학적 분해 실험에 적용하였다. 영양염류 및 산소 농도, 균농도 등 최적의 환경인자 도출을 통해 최적의 생물학적 처리 효율을 TPH의 경우, MSM 및 activator I을 주입하여 25일 동안 76.3%의 제거 효율과 제거속도상수 K=0.711를 나타냈으며, diesel의 경우 40일 동안 99.2%의 제거 효율을 보였다. 또한, TPH 오염 토양의 lab-scale bioremediation 실험에서 고효율 균주를 적용한 결과 45일 운전 기간 동안7,209.9 mg·kg^(-1)을 825.6 mg·kg^(-1)까지 88.5% 제거하였다. 본 연구에서 도출된 TPH로 오염된 토양의 bioremediation 을 위한 고효율 균주 확보와 최적의 환경 인자 도출은 현재부족한 생물학적 처리 연구와 물리적 화학적 처리의 문제를해소하기 위한 기초적 실험 자료로서 기여할 것으로 사료된다.
Abstract
We previously showed that five strains belonging to Pseudomonas could remove TPH (Total Petroleum Hydrocarbons) efficiently when they are applied to TPH-contaminated soil. We optimized the bioremediation condition using different hydrocarbons and nutrients conditions to improve the efficiency. We setup lab-scale column bioreactor to monitor TPH and diesel removal efficiency. When we applied five Pseudomonas sp. mixtures to 25,000 mg ·kg^(-1) TPH-contaminated soil (diesel 10,000 mg ·kg^(-1),kerosene 10,000 mg·kg^(-1), gasoline 5,000 mg·kg^(-1) with the optimum condition, 76.3% of TPH removal efficiency was shown for 25 days. Meanwhile, in the application of five Pseudomonas sp. mixtures to 20,000mg·kg^(-1) diesel-contaminated soil with the optimum condition, 99.2% of diesel removal efficiency was shown for 40 days. In the application to lab-scale bioreactor with five high efficiency bacteria, 88.5% of TPH removal efficiency was shown for 45 days. Based on the results from this study, we confirmed that this mixed Pseudomonas sp. consortium might improve the bioremediation of TPH in contaminated soil, the efficacy can be controlled by improving the nutrients. We also confirmed that the nutrients and oxygen for biodegradation of TPH could contribute on the management and control of applications of these strains for the study of bioremediation of TPH-contaminated soil.
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