디젤 분해 세균 Gordonia sp. SD8 분리 및 특성
Isolation and Characterization of a Diesel-Degrading Bacterium, Gordonia sp. SD8.
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본 연구에서는 디젤로 오염된 토양에서 디젤 분해능이 우수한 Gordonia sp. SD8을 분리하였고, 이 균주의 디젤 분해특성을 액상과 토양에서 조사하였다. SD8은 유일 에너지원과 탄소원으로 디젤을 이용하여 생장 가능하였다. SD8 균주의 성장과 디젤 분해속도에 미치는 디젤 농도 영향을 조사한 결과, 20,000 mg-TPH L-1농도에서 최대 비성장속도(0.67±0.05 d-1)와 최대분해속도(1727±145 mg-TPH L-1 d-1)를 얻을 수 있었다. 또한, 이 균주는 40,000 mg-TPH L-1의 고농도 디젤을 분해할 수 있었으며, 30oC에서 비성장속도와 디젤분해속도가 가장 빨랐다. 디젤로 오염된 토양 정화에 미치는 Gordonia sp. SD8 접종 효과를 조사한 결과, 17일 경과 후, SD8을 접종하지 않은 대조군 토양의 디젤 잔류 농도는 8,150±755 mg-TPH kg-dry soil-1이었으나, SD8을 접종한 경우에는 3,724 mg-TPH kg-dry soil-1이었다. 이러한 결과는 Gordonia sp. SD8는 향후 디젤 등을 포함한 석유계 탄화수소화합물로 오염된 토양을 정화하는데 활용 가능한 유용 미생물 자원임을 의미한다.
Abstract
A diesel-degrading bacterium, Gordonia sp. SD8, was isolated from soil contaminated with petroleum, and its diesel degradation was characterized in a soil as well as a liquid culture system. SD8 could grow in the mineral salt medium supplemented with diesel as a sole carbon and energy source. The maximum specific growth rate (0.67±0.05 d-1) and diesel degradation rate (1,727±145 mg-TPH L-1d-1) of SD8 showed at 20,000 mg-TPH L-1 and 30oC, and then this bacterium could degrade high strength of diesel of 40,000 mg-TPH L-1. The residual diesel concentration in the inoculated soil with SD8 was 3,724 mg-TPH kg-dry soil-1 after 17 days, whereas the diesel concentration in the non-inoculated soil was 8,150±755mg-TPH kg-dry soil-1. These results indicate that Gordonia sp. SD8 can serve as a promising microbial resource for the bioremediaion of contaminated soil with petroleum hydrocarbons including diesel.
참고문헌 (31)
- Arenskötter, M, "Biology of the metabolically diverse genus Gordonia", Appl. Environ. Microbiol., 70, pp.3195~3204 (2004)
- Ashok, B. T, "Isolation and characterization of four polycyclic aromatic hydrocarbon degrading bacteria from soil near an oil refinery", Lett. Appl. Microbiol., 21, pp.246~248 (1995)
- Bento, F. M, "Comparative bioremediation of soils contaminated by natural attenuation, biostimulation and bioaugmentation", Bioresour. Technol., 96, pp.1049~1055 (2005)
- Dean-Ross, D, "Utilisation of mixtures of Polycyclic aromatic hydrocarbons by bacteria isolated from contaminated sediment", FEMS Microbiol. Ecol., 41, pp.1~7 (2002)
- Franzetti, A, "Surface-active compounds and their role in the access to hydrocarbons in Gordonia strains", FEMS Microbiol. Ecol., 63, pp.238~248 (2008)
- Franzetti, A, "Potential applications of surface active compounds by Gordonia sp. strain BS29 in soil remediation technologies", Chemosphere, 75, pp.801~807 (2009)
- Haritash A. K, "Biodegradation aspects of Polycyclic Aromatic Hydrocarbons (PAHs): A review", J. Hazard. Mater., 169, pp.1~15 (2009)
- Hong, J. H, "Characterization of a diesel-degrading bacterium, Pseudomonas aeruginosa IU5, isolated from oil-contaminated soil in Korea", World J. Microb. Biot., 21, pp.381~384 (2004)
- Huang, X. D, "A multi-process phytoremediation system for decontamination of persistent total petroleum hydrocarbons(TPHs) from soils", Microchem. J., 91, pp.139~147 (2005)
- Koo, S. Y, "Isolation and characterization of a plant growth promoting rhizobacterium, Serratia sp. SY5", J. Microbiol. Biotechn., 19, pp.1431~1438
- Lee, E. H, "Effect of substrate interaction on the degradation of methyl tert-butyl ether, benzene, toluene, ethylbenzene, and xylene by Rhodococcus sp", J. Hazard. Mater., 167, pp.669~674 (2009)
- Lee, E. H, "Degradation of hexane and other recalcitrant hydrocarbons by a novel isolate, Rhodococcus sp. EH831", Environ. Sci. Pollut. Res., 17, pp.64~77 (2009)
- Lee, J. J, "Degradation of 3- Methylpyridine and 3-Ethylpyridine by Gordonia nitida LE31", Appl. Environ. Microbiol, 67, pp.4342~4345 (2001)
- Liu, L., "Isolation and identification of a PAHs-degrading strain Gordonia sp. He4 and its dynamics during bioremediation of phenanthrene polluted soil", Huan. Jing. Ke. Xue., 28, pp.617~622 (2007)
- Macek, T. M, "Exploitation of plants for the removal of organics in environmental remediation(research review paper)", Biotechnol. Adv., 18, pp.23~34 (2000)
- Margesin, R, "Characterization of hydrocarbon-degrading microbial populations in contaminated and pristine Alpine soils", Appl. Environ. Microbiol., 69, pp.3085~3092 (2003)
- Margesin, R, "Bioremediation (Natural Attenuation and Biostimulation) of Diesel-Oil-Contaminated Soil in an Alpine Glacier Skiing Area", Appl. Environ. Microbiol., 67, pp.3127~3133 (2001)
- Alvarez, Vanessa Marques, "Bioremediation Potential of a Tropical Soil Contaminated with a Mixture of Crude Oil and Production Water", Journal of Microbiology and Biotechnology, 18(12), pp.1966~1974 (2008), 한국미생물·생명공학회
- Medina-Bellver, J. I, "Evidence for in situ crude oil biodegradation after the Prestige oil spill", Environ. Microbiol., 7, pp.773~779 (2005)
- Philp, J. C, "Environmental pollution and restoration: A role for bioremediation. in Bioremediation", pp.1~48 (2005), ASM Press
- Prince, R. C, "Substrate Preferences in Biodesulfurization of Diesel Range Fuels by Rhodococcus sp. Strain ECRD-1", Appl. Environ. Microbiol., 69, pp.5833~5838 (2003)
- Rehmann, K, "Pyrene degradation by Mycobacterium sp. Strain KR2", Chemosphere, 36, pp.2977~2992 (1998)
- Reichenauer, T. G, "Phytoremediation of Organic Contaminants in Soil and Groundwater", Chem. Sus., 1, pp.708~717 (2008)
- Romero, M. C, "Phenanthrene degradation by microorganisms isolated from a contaminated stream", Environ. Pollut., 101, pp.355~359 (1998)
- Tam, N. F. Y, "Preliminary study on biodegradation of phenanthrene by bacteria isolated from mangrove sediments in Hong-Kong", Mar. Poll. Bull., 42, pp.316~324 (2002)
- Torsvik, V, "Prokaryotic diversity-magnitude, dynamics, and controlling factors", Science, 296, pp.1064~1066 (2002)
- Valeria, P. A, "Biodegradation of effluent contaminated with diesel fuel and gasoline", J. Hazard. Mater., 140, pp.52~59 (2007)
- Van Hamme, J. D, "Physical and metabolic interactions of Pseudomonas sp. strain JA5-B45 and Rhodococcus sp. strain F9-D79 during growth on crude oil and effect of a chemical surfactant on them", Appl.Environ. Microbiol, 67, pp.4874~4879 (2003)
- Watanabe,K, "Microorganisms relevant to bioremediation", Curr. Opin. Biotechnol., 12, pp.237~241 (2001)
- Wei, Q. F, "Oil removal from used sorbents using a biosurfactant", Bioresour. Technol., 96, pp.331~334 (2005)
- Yuan, S. Y, "Biodegradation of polycyclic aromatic hydrocarbons by inoculated microorganisms in soil", Bull. Environ. Contam. Toxicol., 69, pp.66~73 (2002)