Analysis of coenzyme Q10 in human plasma by high performance liquid chromatography
0
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Coenzyme Q10(CoQ10), a vitamin E-like substance, represents a components of the complex antioxidant system of the human organism. CoQ10 levels in human plasma were determined by high performance liquid chromatography (HPLC) with UV detection. It was dissociated from lipoproteins by methanol and extracted into n-hexane with liquid-liquid extraction procedure, after centrifugation, the supernatant was dried under nitrogen gas stream. The residue was dissolved in the absolute ethanol. Determination of CoQ10 was performed on a C18 reversed-phase analytical column with ultraviolet detection at 275 nm and the mobile phase containing 15% (v/v) ethanol in methanol at a flow rate of 1.7 mL/min. The low limit of quantitation was 0.02 mg/L (S/N=10), the linearity between the concentration and peak height is from 0.1 to 2.0 mg/L. Twenty-four randomly selected plasma samples from apparently healthy, 27 to 44 year old individuals (males and females) were analyzed for total CoQ10. The average level in these subjects was 0.62±0.13 mg/L with the range of 0.41- 0.98 mg/L. This method has a specific and a sufficient limit of quantitation (LOQ) for analysis of CoQ10 in human plasma in both a clinical study and research at laboratories
Abstract
Coenzyme Q10(CoQ10), a vitamin E-like substance, represents a components of the complex antioxidant system of the human organism. CoQ10 levels in human plasma were determined by high performance liquid chromatography (HPLC) with UV detection. It was dissociated from lipoproteins by methanol and extracted into n-hexane with liquid-liquid extraction procedure, after centrifugation, the supernatant was dried under nitrogen gas stream. The residue was dissolved in the absolute ethanol. Determination of CoQ10 was performed on a C18 reversed-phase analytical column with ultraviolet detection at 275 nm and the mobile phase containing 15% (v/v) ethanol in methanol at a flow rate of 1.7 mL/min. The low limit of quantitation was 0.02 mg/L (S/N=10), the linearity between the concentration and peak height is from 0.1 to 2.0 mg/L. Twenty-four randomly selected plasma samples from apparently healthy, 27 to 44 year old individuals (males and females) were analyzed for total CoQ10. The average level in these subjects was 0.62±0.13 mg/L with the range of 0.41- 0.98 mg/L. This method has a specific and a sufficient limit of quantitation (LOQ) for analysis of CoQ10 in human plasma in both a clinical study and research at laboratories
참고문헌 (17)
- L. Ernster, "Bioimedical and Clinical Aspects of Coenzyme Q", pp.1977, Amsterdam, Elsevier
- W. A. Cramer, "Energy Transduction in Biological Membranes" (1990), Springer Verlag, Berlin
- B. L, Trumpower, "Function of Quinones in Energy Conservation Systems" (1982), Academic Press
- P. Mitchell, "The vital protomnotive role of coenzyme Q In : Biomedical and Clinical Aspects of Coenzyme Q" (1991), Amsterdam
- J. Kaikkonen, , Clin Lab. Invest, 59(6), pp.457~466 (1999)
- S. Hodges, , Biofactors, 9, pp.365~370 (1999)
- M. Takada, , Biochim. Biophys. Acta, (679), pp.308~314 (1982)
- A. Zhiri, , J. Liq. Chromatogr, 17, pp.2633~2640 (1994)
- M. A. Pacanowski, , J. Clin Lipidology, 2, pp.289~297 (2008)
- J. Karpinska, , J. Pharm and Biomedical Analysis, 17, pp.1345~1350 (1998)
- P. H. Tang, , Clin Chem., 47(2), pp.256~265 (2001)
- W. K. Lu, , Biol. Pharm. Bull., 26(1), pp.52~55 (2003)
- K. Aikkarach, , J. Clin Biochem Nurt., 40, pp.194~202 (2007)
- J. Kucharska, , Physiol. Res., 47, pp.399~404 (1998)
- P. Kaplan, , Physiol, Res., 44, pp.39~45 (1995)
- K. Jonansen, , Ann. Med, 23, pp.649~656 (1991)
- "Skeletal muscle coenzyme Q10 in healthy man and selected patient groups. In: Biomedical and Clinical Aspects of Coenzyme Q", pp.191~204 (1991), Elsevier Science Publishers. Amsterdam