HPLC/UV를 이용한 혈장 중 이트라코나졸의 분석
Deternubatuib if traconazole in human plasma by high performance liquid chromatography
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본 연구는 LLE(liquid-liquid extraction) 전처리 방법과 HPLC/UV를 이용하여 혈장에서 이트라코 나졸을 정량 분석하는 방법을 연구하였다. 이트라코나졸과 내부표준물질(펠로디핀)을 디에틸에테르로 추 출하고 C18 컬럼을 이용하여 분리한 후 UV 검출기를 통하여 254 nm에서 검출하였다. 이동상은 10 mM 아세트산 암모늄 완충액(pH 7) :아세토나이트릴(35:65, v/v) 혼합용액을 사용하였으며, 유속은 0.2 mL/min. 으로 하였다. 정량범위는 2~1,000 ng/mL 이며, 상관관계(R2)는 0.9991로 좋은 직선성을 보였다. 정량한계 는 2 ng/mL, 재현성은 변동계수가 10.8 이하, 정확도는 97.2~108.2% 였다. 이 분석방법은 혈장 중의 약 물 정량 및 약물의 약동력학에 유용함을 보여주었다.
Abstract
This method is used for the determination of itraconazole in human plasma by liquid-liquid extractionand high performance liquid chromatography. Felodipine was used as an internal standard. After extraction ofthe plasma with diethyl ether, the centrifuged upper layer was then transferred. The supernantant was evaporatedand then reconsituted with mobile phase. The mobile phase was composed of 10 mM ammonium acetate adjustedto pH 7 by phosphoric acid with a flow rate of 0.2 mL/min. A C18 reversed-phase column with a pre-columnfor 2~1,000 ng/mL. The correlation coefficient of linear regression(R2) was 0.9991, limit of quantification (LOQ)was 2 ng/mL, reproducibility was less than 10.8 %, and accuracy was 97.2~108.2%. This method has beensuccessfully applied to the pharmacokinetic study of itraconazole in human plasma.
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