바이오-전기화학 융합기술 기반 NADH 재생 시스템 연구
Efficient NADH Regeneration by a Bioelectrocatalytic Enzymatic System
1
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Efficient NADH regeneration from its oxidized form (NAD+) is required to support enzyme-based organic synthesis and the pharmaceutical industry. Prominent industrial NADH regeneration methods have shown promise; however, require the continuous addition of substrate, requiring downstream separation and purification. In this study, the utilization of diaphorase which can directly reduce the NAD+to NADH, with strong and stable mediator is presented which is capable of producing bioactive 1,4-NADH, faradaic efficiencies ~83% at varying temperature (20 to 60 °C). The efficiency of this approach as well as its high operational stability (87% of the maximum activity after six days) make it promising strategy for NADH regeneration.
Abstract
Efficient NADH regeneration from its oxidized form (NAD+) is required to support enzyme-based organic synthesis and the pharmaceutical industry. Prominent industrial NADH regeneration methods have shown promise; however, require the continuous addition of substrate, requiring downstream separation and purification. In this study, the utilization of diaphorase which can directly reduce the NAD+to NADH, with strong and stable mediator is presented which is capable of producing bioactive 1,4-NADH, faradaic efficiencies ~83% at varying temperature (20 to 60 °C). The efficiency of this approach as well as its high operational stability (87% of the maximum activity after six days) make it promising strategy for NADH regeneration.
참고문헌 (23)
- Wenshuo W., "Hydride Transfer for NADH Regeneration: From Nature, Beyond Nature", Adv. Ener. Sus. Res, 4(3), pp.2200172~2200174 (2023)
- Ryu J., "Rational Design and Engineering of Quantum-Dot-Sensitized TiO2 Nanotube Arrays for Artificial Photosynthesis", Adv. Mater., 23(16), pp.1883~1888 (2011)
- Morrison C. S., "Improved Strategies for Electrochemical 1,4-NAD(P)H2Regeneration: A New Era of Bioreactors for Industrial Biocatalysis", Biotechnol. Adv., 36(1), pp.120~131 (2018)
- Kim S. H., "Electrochemical NADH Regeneration and Electroenzymatic CO2 Reduction on Cu Nanorods", Glassy Carbon Electrode Prepared by Cyclic Deposition, 2016, pp.210(20), 837~845
- Wu H., "Methods for the Regeneration of Nicotinamide Coenzymes", Green Chem, 15(7), pp.1773~1789 (2013)
- Yuanyuan Z., "Bioinspired NADH regeneration based on conjugated photocatalytic systems", Sol. RRL, 5(2), pp.2000339~2000342 (2021)
- Hollmann F., "Biocatalytic Redox Reactions for Organic Synthesis: Nonconventional Regeneration Methods", ChemCatChem, 2(7), pp.762~782 (2010)
- Lucy BF B., "Controlled Biocatalytic Synthesis of a Metal Nanoparticle‐Enzyme Hybrid: Demonstration for Catalytic H2‐driven NADH Recycling", Ang. Chem. Int. Edition, pp.e202404024~ (2024)
- Baik, S. H., "Direct Electrochemical Regeneration of Nadh from Nad+ Using Cholesterol-Modified Gold Amalgam Electrode", Biotechnol. Tech, 13, pp.1~5 (1999)
- Barin, R., "Direct Electrochemical Regeneration of 1, 4-Nadh at the Copper Foam and Bimetallic Copper Foam", Electrochim. Acta, 247, pp.1095~1102 (2017)
- Betanzos-Lara S., "Organometallic Ruthenium and Iridium Transfer-Hydrogenation Catalysts Using Coenzyme NADH as a Cofactor", Angew. Chem. Int. Ed. Engl, 51(16), pp.3897~3900 (2012)
- 2+ and Enzymes−Analysis and Potential Remedies, "J. Mol. Catal. B: Enzym", 2010, 149~156
- Zhang L., "Covalent Immobilization of (2, 2 ′-Bipyridyl)(Pentamethylcyclopentadienyl)-Rhodiu m Complex on a Porous Carbon Electrode for Efficient Electrocatalytic NADH Regeneration", ACS Catal, 7(7), pp.4386~4394 (2017)
- Kun D, "Electricity production and key exoelectrogens in a mixed-culture psychrophilic microbial fuel cell at 4° C", Appl. Microb. Biotech, 106(12), pp.4801~4811 (2022)
- Schmid A., "The Use of Enzymes in the Chemical Industry in Europe", Curr. Opin. Biotechnol, 13(4), pp.359~366 (2002)
- Complexes for NADH Regeneration, "Inorg. Chem.", 2017, 1366~1374
- Yaoxuan L., "Electrocatalytic NAD(P)H regeneration for biosynthesis", Green Chem. Eng, 5(1), pp.1~15 (2024)
- Mizue W., "Significance of nano-structures of carbon materials for direct-electron-transfer-type bioelectrocatalysis of bilirubin oxidase", Electrochem, 88(5), pp.374~379 (2020)
- Dinh T. H., "Diaphorase-Viologen Conjugates as Bioelectrocatalysts for NADH Regeneration", J. Electrochem. Soc., 163(6), pp.H440~H444 (2016)
- Ren L., "Synthesis and Solution Self-Assembly of Side-Chain Cobaltocenium-Containing Block Copolymers", J. Am. Chem. Soc, 132(26), pp.8874~8875 (2010)
- Wang X., "Heterogeneous Catalysis Mediated Cofactor NADH Regeneration for Enzymatic Reduction", ACS Catal, 6(3), pp.1880~1886 (2016)
- Damian A., "Direct Electrochemical Regeneration of NADH on Au, Cu and Pt-Au Electrodes", Chem. Biochem. Eng. Q, 21(1), pp.21~32 (2007)
- Yunling B., "Production and separation of formate dehydrogenase from Candida boidinii", Enzyme microb. technol, 40(4), pp.940~946 (2007)