철 화합물을 이용한 당 탈수소화 효소-혈당센서의 연구
Development of GDH-glucose Sensor using Ferrate Complex
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산화/환원 매개체는 혈당 센서의 구성에서 전극과 효소 반응의 전자 전달 매개체로서 중요한 역할을 담당한다. 본 연구에서는 기존의 산화/환원 매개체보다 전자 전달 반응이 용이하며, 높은민감도를 위해 페레이트에 아닐린을 결합시켜, 1차 아민기를 갖는 Fe(CN)5-aminopyridine를 합성하였다. 합성된 Fe(CN)5-aminopyridine 는 순환 전압 전류 법과 분광학적 방법을 이용하여합성 결과를 확인하였다. 합성된 물질과 포도당을 측정하기 위한 당 탈 수소 효소를 ITO 전극위에 고정시켜 효소전극을 제작하였고, 또한 신호 증폭을 위하여 금 나노 입자를 함께 고정시켰다. 금 나노 입자가 고정된 효소 전극은 그렇지 않은 전극에 비해 약 2배 가량의 전류 밀도가증가함을 확인하였다. 만들어진 효소 전극에서 포도당의 농도 별 산화 촉매 전류를 순환 전압전류 법으로 측정한 결과 0.4 V (vs. Ag/AgCl)에서 전기적 신호가 발생되었으며, 포도당0~10 mM의 농도 범위에서 전기적 신호가 선형 증가함을 확인할 수 있었다.
Abstract
Redox complexes to transport electrons from enzyme to electrodes are very importantpart in glucose sensor. Pentacyanoferrate-bound aniline (Fe(CN)5-aminopyridine), was preparedas a potential redox mediator in a glucose dehydrogenase (GDH)-glucose sensor. Thesynthesized pyridyl-NH2 to pentacyanoferrate was characterized by the electrochemical andspectroscopic methods. A amperometric enzyme-linked electrode was developed based onGDH, which catalyses the oxidation of glucose. Glucose was detected using GDH that wasco-immobilized with an Fe(CN)5-aminopyridine and gold nano-particles (AuNPs) on ITO electrodes. The Fe(CN)5-aminopyridine and AuNPs immobilized onto ITO electrodes providedabout a two times higher electrochemical response compared to that of a bare ITO electrode. As glucose was catalyzed by wired GDH, the electrical signal was monitored at 0.4 V versusAg/AgCl by cyclic voltammetry. The anode currents was linearly increased in proportion tothe glucose concentration over the 0~10 mM range.
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