웅성불임 인자를 이용한 갓(Brassica juncea L. Czern)의 F1 육종
Breeding of F1 Hybrid for Oriental Mustard(Brassica juncea L. Czern) Using the Cytoplasmic Male Sterile Line
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초록
김치가 세계 5대 건강식품으로 선정되어 세계인의 주목을 받게 되면서 갓의 수요도 점점 증가하고있어 다양한 갓품종의 육성이 절실히 요구되는 실정이다. 그러나 아직 품종보급 체계가 미흡하여 자가채종에 의한 자식약세현상, 순도저하, 상품의 불균일 등의 문제점이 발생하고 있다. 본 실험에서는 갓의 F1 품종 육성체제를 갖추기 위하여 유전자원으로 수집한 갓의 순계분리와 자식계통의 육성, 웅성불임인자의 도입을 통하여 고품질의 갓을 육성하기 위하여 각 조합을 공시하여 조합능력을 검정하고(인도자사이×고흥담양)조합과 (분리 MS 유기계-MS910×일본 적자색갓 8×강화갓 9) 분리계 조합이 우수하다고 판단되어 양친으로 선발하였다. PCR(Polymerase Chain Reaction)과 염기서열 분석을 통해CMS type을 확인해 본 결과, MS계통 MSLS와 MSLC는 갓에서 보고된 orf263, orf220 및 orf288CMS 유전자를 미토콘드리아 DNA에 포함하고 있는 것으로 나타났다. 최종적으로 생산력검정 및 농가실증시험을 실시하였다.
Abstract
Recently, the demand for new cultivar of oriental mustard(Brassica juncea) is increased as theconsumption of oriental mustard has increased dramatically in the market due to Kimchi isattracting the world's attention. However, absence of seed supply system can cause manyproblems including inbreeding depression due to self seed production, deterioration of the seedpurity and heterogeneity of commercial seed. To establish the F1 variety breeding system oforiental mustard, pure line and inbred lines were screened from inbreeding genetic resources. Male-sterile line was also selected from breeding combinations for the quality improvement ofmustard. The combining ability from(Indojasai × Goheungdamyang) combinations and isolationline of(MS910 × Japan red mustard 8 × Ganghwa mustard 9) was highest, thus these lineswere selected as parental lines. PCR(Polymerase Chain Reaction) and gene sequence analysisrevealed that the genes related to CMS(orf263, orf220, and orf288) were distributed inmitochondria. The isolated lines from this study also showed good performance in yield test andfarmhouse prove test.
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