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Evaluation of Cytoplasm types by PCR-marker in Male-Sterility of Onion (Allium cepa L.)

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The production of hybrid onion (Allium cepa L.) seed is economically feasible using systems of cytoplasmic-genic male sterility(CMS). The identification of cytoplasm types might be helpful to development of sterile and maintainer lines. Due to this reason, a knowledge of the cytoplasm type is essential for hybrid breeding in onion. Especially, due to the biennial generation time of onion, to distinguish of onion cytoplasm type in individual plant may be helpful to reduce the efforts in a breeding program, when new sterile lines and the corresponding maintainer lines be developed. In our study, we applied the PCR-marker (orfA-501) to identify the cytoplasmic genotypes of collected 100 accessions of bulb onion. Among accessions, S-cytoplasm was found in 57 accessions. Nineteen accessions possessed only N-cytoplasm and twenty four accessions possessed both S- and N-cytoplasm.

Abstract

The production of hybrid onion (Allium cepa L.) seed is economically feasible using systems of cytoplasmic-genic male sterility(CMS). The identification of cytoplasm types might be helpful to development of sterile and maintainer lines. Due to this reason, a knowledge of the cytoplasm type is essential for hybrid breeding in onion. Especially, due to the biennial generation time of onion, to distinguish of onion cytoplasm type in individual plant may be helpful to reduce the efforts in a breeding program, when new sterile lines and the corresponding maintainer lines be developed. In our study, we applied the PCR-marker (orfA-501) to identify the cytoplasmic genotypes of collected 100 accessions of bulb onion. Among accessions, S-cytoplasm was found in 57 accessions. Nineteen accessions possessed only N-cytoplasm and twenty four accessions possessed both S- and N-cytoplasm.

참고문헌 (13)

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  12. specific mitocondrial nucleotide sequences to identify cytoplasmic genotypes of onion (Allium cepa L.), , 1998, 367~370
  13. Nagai M, "The use of mitocondrial DNA polymorphism in the classification of the individual onion plants by the cytoplamic genotypes"

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