SWCNT 및 MWCNT의 기관내 점적주입 후 폐 계면활성제의 분석
Analysis of Pulmonary Surfactant after Intratracheal Instillation of SWNCT and MWCNT
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Objectives: Carbon nanotubes (CNTs) are next-generation industrial nanoparticles which possess excellent mechanical strength along with good thermal conductivity and electric properties. Given these characteristics, carbon nanotubes are being widely applied in various fields, including research and development. However, concerns have been raised over hazardous properties due to their similar fiber shape to asbestos. Recent studies have shown that CNTs pose potential hazards which may cause fibrosis and/or lung inflammation similarly to asbestos. Methods: After intratracheal instillation of SWCNTs and MWCNTs to rats, pulmonary surfactant (PS) of the SWCNTs and MWCNTs was measured and analyzed using bronchoalveolar lavage fluid collected from the lung. After a single intratracheal instillation of SWCNTs and MWCNTs, phospholipid predominantly showed a significant increase compared to the control group, while proteins exhibited a significant increase both three days and one week after instillation. Results: As a result of surface tension, MWCNTs showed a significant decrease three days after treatment compared to the control group. In the case of the total cell number three days after instillation, MWCNTs revealed a temporarily significant increase when compared to the control group. For PMN number, when compared to the control group, SWCNTs displayed a significant increase throughout the observation period, while MWCNTs showed a significant increase three days and three months after treatment. Conclusions: After exposure to CNTs, the total cell number and PNT number, which indicate inflammatory response, were significantly increased. Therefore, this study suggests fiber–shaped CNTs may have a harmful effect on the lungs.
Abstract
Objectives: Carbon nanotubes (CNTs) are next-generation industrial nanoparticles which possess excellent mechanical strength along with good thermal conductivity and electric properties. Given these characteristics, carbon nanotubes are being widely applied in various fields, including research and development. However, concerns have been raised over hazardous properties due to their similar fiber shape to asbestos. Recent studies have shown that CNTs pose potential hazards which may cause fibrosis and/or lung inflammation similarly to asbestos. Methods: After intratracheal instillation of SWCNTs and MWCNTs to rats, pulmonary surfactant (PS) of the SWCNTs and MWCNTs was measured and analyzed using bronchoalveolar lavage fluid collected from the lung. After a single intratracheal instillation of SWCNTs and MWCNTs, phospholipid predominantly showed a significant increase compared to the control group, while proteins exhibited a significant increase both three days and one week after instillation. Results: As a result of surface tension, MWCNTs showed a significant decrease three days after treatment compared to the control group. In the case of the total cell number three days after instillation, MWCNTs revealed a temporarily significant increase when compared to the control group. For PMN number, when compared to the control group, SWCNTs displayed a significant increase throughout the observation period, while MWCNTs showed a significant increase three days and three months after treatment. Conclusions: After exposure to CNTs, the total cell number and PNT number, which indicate inflammatory response, were significantly increased. Therefore, this study suggests fiber–shaped CNTs may have a harmful effect on the lungs.
참고문헌 (22)
- Maynard AD, "Nanotechnology: the next big thing, or much ado about nothing?", Ann Occup Hyg, 51, pp.1~12 (2007)
- Oh KH, "Standardization trends and technologies for carbon nanotubes", JIES, 12(4), pp.20~25 (2009)
- Aiso S, "Pulmonary toxicity of intratracheally instilled multiwall carbon nanotubes in male Fischer 344 rats", Ind Health, 48, pp.783~795 (2010)
- DeLorme MP, "Ninety-day inhalation toxicity study with a vapor grown carbon nanofiber in rats", Toxicol Sci, 128, pp.449~460 (2012)
- Ma-Hock, "Inhalation toxicity of multi-wall carbon nanotubes in rats exposed for three month", Toxicol Sci, 112, pp.468~481 (2009)
- Mitchell LA, "Pulmonary and systemic immune response to inhaled multiwalled carbon nanotubes", Toxicol Sci, 100, pp.203~214 (2007)
- Muller J, "Absence of carcinogenic response to multiwall carbon nanotubes in a 2-year bioassay in the peritoneal cavity of the rat", Toxicol Sci, 110, pp.442~448 (2009)
- Pauluhn J, "Subchronic 13week inhalation exposure of rats to multiwalled carbon nanotubes: toxic effects are determined by density of agglomerate structures, not fibrillar structures", Toxicol Sci, 113, pp.226~242 (2010)
- Lam CW, "Pulmonary toxicity of single-wall carbon nanotubes in mice 7 and 90days after intratracheal instillation", Toxicol Sci, 77(1), pp.126~134 (2004)
- Guang Jia, "Cytotoxicity of carbon nanomaterials: Single-wall nanotube, multi-wall nanotube, and fullerene", Environ Sci Technol, 39(5), pp.1378~1383 (2005)
- Risk assessment of manufactured nanomaterials: Approaches- overview of approaches and results, in Research and development of nanoparticle characterization methods, "Nakanishi J", NEDO, ~ (2011)
- Kobayashi N, "Biological response and morphological assessment of individually dispersed multiwall carbon nanotubes in the lung after intratracheal instillation in rats", Toxicology, 276, pp.143~153 (2010)
- Morimoto Y, "Pulmonary toxicity of well-dispersed multi-wall carbon nanotubes following inhalation and intratracheal instillation", Nanotoxicology, 6, pp.587~599 (2011)
- Schleh C, "Interaction of nanoparticles with the pulmonary surfactant system", Inhal Toxicol, 21, pp.97~103 (2009)
- Kadoya C, "Analysis of bronchoalveolar lavage fluid adhering to lung surfactant", Ind Health, 50, pp.31~36 (2012)
- 최용성, "신생아 호흡곤란증후군에서 인공 폐표면활성제의 사용", 대한의사협회지, 58(4), pp.330~335 (2015), 대한의사협회[DOI]
- 나주옥, "한국인에서 만성폐쇄성폐질환과 인체 폐 표면 활성제 단백-A 유전자 대립형질의 상관관계", Tuberculosis and Respiratory Diseases, 60(6), pp.638~644 (2006), 대한결핵및호흡기학회
- Shvedova AA, "Unusual inflammatory and fibrogenic pulmonary responses to single walled carbon nanotubes in mice", Am J Physiol Lung Cell Mol Physiol, 289, pp.698~708 (2005)
- Li JG, "Comparative study of pathological lesions induced by multiwalled carbon nanotubes in lungs of mice by intratracheal instillation and inhalation", Environ Toxicol, 22(4), pp.415~421 (2007)
- Cunningham, MJ, "Gene-cellular interactions of nanomaterials: genotoxicity to genomics in Nanotoxicoloy-Characterization, Dosing and Health Effects", pp.173~196 (2007), Informa healthcare
- Muller J, "Clastogenic and aneugenic effects of multi-wall carbon nanotubes in epithelial cells", Carcinogenesis, 29, pp.427~433 (2008)
- Tanaka Isamu, "Evaluating Risks associated with Manufactured Nanomaterials. Developing Toxicity Evaluating Methods by the Inhalation Exposure", ~ (2006)