PTU 투여 갑상선기능저하증 유발 랫드모델에서 고용량 MOK 약침의 효능 및 안전성 평가 연구
Study on Efficacy and Safety of High Dose MOK Pharmacopuncture in Hypothyroidism-induced Rats with Propylthiouracil
0
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
In this study, we investigated the effects of MOK pharmacopuncture at high-doses which are increased 10 to 100-fold in clinics, on propylthiouracil (PTU)-induced hypothyroidism in rats and the safety. We measured the changes of body weight, food and water intake, body temperature, the serum levels of thyroid hormones (TSH, T3, and T4), AST and ALT, glucose, lipid metabolites (total cholesterol, HDL-cholesterol, LDL-cholesterol, and triglyceride) and observed histopathological changes of thyroid tissues by H&E staining. We also analyzed the peaks of constituents of MOK using HPLC. In the results, the treatment of MOK pharmacopuncture at high-dose (30 mg/kg) in hypothyroidism-induced rats for 2 weeks was shown the improvement effects on the decrease of body weight, food intake, and body temperature, The MOK pharmacopunture at high dose regulated the imbalance of thyroid hormones, glucose, and lipid metabolites and also inhibited the structural damages of thyroid tissues. In liver damage, the MOK pharmacopuncture at high dose reduced the increase of AST and ALT levels in hypothyroid rats. We identified the MOK constituents in HPLC analysis. In conclusion, the treatment of MOK pharmacopuncture at high dose has a therapeutic effect on hypothyroidism without liver toxicity, suggesting that the MOK pharmacopuncture be usefully applicable to treat with hypothyroidism in clinics.
Abstract
In this study, we investigated the effects of MOK pharmacopuncture at high-doses which are increased 10 to 100-fold in clinics, on propylthiouracil (PTU)-induced hypothyroidism in rats and the safety. We measured the changes of body weight, food and water intake, body temperature, the serum levels of thyroid hormones (TSH, T3, and T4), AST and ALT, glucose, lipid metabolites (total cholesterol, HDL-cholesterol, LDL-cholesterol, and triglyceride) and observed histopathological changes of thyroid tissues by H&E staining. We also analyzed the peaks of constituents of MOK using HPLC. In the results, the treatment of MOK pharmacopuncture at high-dose (30 mg/kg) in hypothyroidism-induced rats for 2 weeks was shown the improvement effects on the decrease of body weight, food intake, and body temperature, The MOK pharmacopunture at high dose regulated the imbalance of thyroid hormones, glucose, and lipid metabolites and also inhibited the structural damages of thyroid tissues. In liver damage, the MOK pharmacopuncture at high dose reduced the increase of AST and ALT levels in hypothyroid rats. We identified the MOK constituents in HPLC analysis. In conclusion, the treatment of MOK pharmacopuncture at high dose has a therapeutic effect on hypothyroidism without liver toxicity, suggesting that the MOK pharmacopuncture be usefully applicable to treat with hypothyroidism in clinics.
참고문헌 (27)
- Doo HK., "Oriental Medicine of Nephrology" (1993), The Academy of oriental Medicine
- Escobar-Morreale HF, "Treatment of hypothyroidism with levothyroxine or a combination of levothyroxine plus L-triiodothyronine", Best Pract Res Clin Endocrinol Metab, 29(1), pp.57~75 (2015)
- Cho BY, "The clinical thyroidology", pp.437~476 (2010), Korea Medicine
- 이민혜, "2000년 이후 갑상선 기능저하증에 대한 국내 한의학 실험연구 체계적 고찰", 대한한방내과학회지, 36(4), pp.570~581 (2015), 대한한방내과학회
- 이민혜, "갑상선 기능항진증에 대한 국내 한의약 실험연구 고찰- 2000년 이후", 대한한방내과학회지, 37(6), pp.949~960 (2016), 대한한방내과학회
- 황민섭, "포부자추출물의 갑상선기능저하증 흰쥐모델에서의 한열조절작용에 의한 개선효능 연구", 대한본초학회지, 31(6), pp.63~71 (2016), 대한본초학회
- 김승모, "인삼이 PTU (6-n-propyl-2-thiouracil)로 유발된 Rat 갑상선 기능저하증에 미치는 영향", 대한본초학회지, 25(3), pp.11~18 (2010), 대한본초학회
- 김승모, "시호가 PTU (6-n-propyl-2-thiouracil)로 유발된 Rat 갑상샘 기능저하증에 미치는 영향", 동의생리병리학회지, 26(5), pp.714~723 (2012), 한의병리학회
- 손윤희, "감궁탕이 면역기능 저하 마우스의 임파구활성에 미치는 영향", 동의생리병리학회지, 18(4), pp.995~1000 (2004), 한의병리학회
- Lee YS, "The Effect of Labiatae Herba Prunellae and Thesium chinese Turczaninow Aqua-acupuncture on Taechung(LR3)in Hyperthyroidism Rats", Journal of Korean Acupuncture & Moxibustion Society, 14(1), pp.494~502 (1997)
- 김경식, "The Effect of Radix Angelicae gigantis aqua-acupuncture on the hypothyroidism induced by thiourea in rats", Journal of Pharmacopuncture, 1(1), pp.53~76 (1997), 대한약침학회[DOI]
- Kim KH, "Effect of Haejo-okho-tang Aqua-acupuncture by Density on the Hyperthyroidism Induced by Sodium Levothyroxine in Rats", Journal of Korean Acupuncture & Moxibustion Society, 12(1), pp.221~235 (1995)
- Kim YS, "A Study of the Effects of Ginseng Radix Aqua-Acupuncture on the Hypothyroidism induced by Thiourea in Rats", Kyunghee Medicine, 19(2), pp.202~210 (1990)
- Li L, "Clinical study on acupuncture and moxibustion for treatment of hypofunction of spleen kidney yang deficiency of thyroid function", master's, pp.2014
- Jung C, "A clinical study of immune pharmacopuncturology" (2011), Kyungrak medical publishing co
- Hwang JH, "MOK, a pharmacopuncture medicine, regulates thyroid dysfunction in L-thyroxine-induced hyperthyroidism in rats through the regulation of oxidation and the TRPV1 ion channel", BMC complementary and alternative medicine, 17(1), pp.535~ (2017)
- Hwang JH, "Therapeutic effects of acupuncture with MOK, a polyherbal medicine, on PTU‑induced hypothyroidism in rats", Experimental and therapeutic medicine, 16(1), pp.310~320 (2018)
- Hwang JH, "Anti-inflammatory and antioxidant effects of MOK, a polyherbal extract, on lipopolysaccharide‑stimulated RAW 264.7 macrophages", International journal of molecular medicine, 43(1), pp.26~36 (2019)
- Jo BY, "Clinical Thytoidology" (2001), Korea Medical Book Publisher
- Roti E, "The use and misuse of thyroid hormone", Endocr Rev, 14(4), pp.401~424 (1993)
- Clyde PW, "Combined levothyroxine plus liothyronine compared with levothyroxine alone in primary hypothyroidism : a randomized controlled trial", JAMA, 290(22), pp.2952~2958 (2003)
- 홍민진, "淫羊藿이 실험적으로 유발된 갑상선 기능저하증 동물모델에 미치는 영향과 안전성", Journal of Pharmacopuncture, 14(4), pp.13~22 (2011), 대한약침학회
- 이승진, "육종용이 PTU로 유발된 갑상선 기능저하증 동물모델에 미치는 영향", 동의생리병리학회지, 25(6), pp.989~995 (2011), 한의병리학회
- 최재영, "PTU(6-n-propyl-2-thiouracil)로 유발된 Rat 갑상선 기능저하증에 미치는 烏梅의 효과", 대한본초학회지, 30(4), pp.109~119 (2015), 대한본초학회
- O'Connor JC, "Evaluation of a 15-day screening assay using intact male rats for identifying steroid biosynthesis inhibitors and thyroid modulators", Toxicol Sci, 69, pp.79~91 (2002)
- Zulewski H, "Estimation of tissue hypothyroidism by a new clinical score : evaluation of patients with various grades of hypothyroidism and controls", J Clin Endocrinol Metab, 82(3), pp.771~776 (1997)
- Werner SC, "The thyroid: a fundamental and clinical text", pp.847~ (1978), Harper & Row;