멜론 유전자원의 원예형질 특성 및 유연관계 분석
Evaluation of horticultural traits and genetic relationship in melon germplasm
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Horticultural traits and genetic relationship were evaluated for 83 melon (Cucumis melo L.) cultivars. Survey of a total of 36 characteristics for seedling, leaf, stem, flower, fruit, and seed and subsequent multiple analysis of variance (MANOVA) were conducted. Principal component analysis (PCA) showed that 8 principle components including fruit weight, fruit length, fruit diameter, cotyledon length, seed diameter, and seed length accounted for 76.3% of the total variance. Cluster analysis of the 83 melon cultivars using average linkage method resulted in 5 clusters at coefficient of 0.7. Cluster I consisted of cultivars with high values for fruit-related traits, Cluster II for soluble solid content, and Cluster V for high ripening rate. Genotyping of the 83 cultivars was conducted using 15 expressed-sequence tagged-simple sequence repeat (EST-SSR) from the Cucurbit Genomics Initiative (ICuGI) database. Analysis of genetic relatedness by UPGMA resulted in 6 clusters. Mantel test indicated that correlation between morphological and genetic distance was very low (r = -0.11).
Abstract
Horticultural traits and genetic relationship were evaluated for 83 melon (Cucumis melo L.) cultivars. Survey of a total of 36 characteristics for seedling, leaf, stem, flower, fruit, and seed and subsequent multiple analysis of variance (MANOVA) were conducted. Principal component analysis (PCA) showed that 8 principle components including fruit weight, fruit length, fruit diameter, cotyledon length, seed diameter, and seed length accounted for 76.3% of the total variance. Cluster analysis of the 83 melon cultivars using average linkage method resulted in 5 clusters at coefficient of 0.7. Cluster I consisted of cultivars with high values for fruit-related traits, Cluster II for soluble solid content, and Cluster V for high ripening rate. Genotyping of the 83 cultivars was conducted using 15 expressed-sequence tagged-simple sequence repeat (EST-SSR) from the Cucurbit Genomics Initiative (ICuGI) database. Analysis of genetic relatedness by UPGMA resulted in 6 clusters. Mantel test indicated that correlation between morphological and genetic distance was very low (r = -0.11).
참고문헌 (19)
- Botstein D, "Construction of a genetic linkage map in man using restriction fragment length polymorphisms", Am. J. Hum. Genet, 32, pp.314~331 (1980)
- Chen Y, "Genetic variation and relationships among cultivated, wild and semi wild soybean", Crop Sci, 44, pp.316~325 (2004)
- 조두현, "AFLP 분석을 통한 국내 떫은감의 유연관계 분석", 원예과학기술지, 25(2), pp.114~118 (2007), 한국원예학회
- Hosaka K, "Genetic relationships of Japanese potato cultivars assayed by RAPD analysis", Amer. J. Potato Res, 71, pp.535~546 (1994)
- Jamshidi S, "NTSYSpc 2.02e implementation in molecular biodata analysis(Clustering, screening and individual selection)" (2011), IACSIT press
- Kim TC, "Classification of persimmon (Diospyros kaki thunb.) cultivars by multivariate analysis", J. Kor Soc. Hort. Sci, 36(4), pp.513~521 (1995)
- Kerje T, "The origin of melon, Cucumis melo: A review of the literature", Acta. Hort, 510, pp.37~44 (2000)
- Yong-Sham Kwon, "Use of SSR Markers to Complement Tests of Distinctiveness,Uniformity, and Stability (DUS) of Pepper (Capsicum annuum L.) Varieties", Molecules and Cells, 19(3), pp.428~435 (2005), 한국분자세포생물학회
- 권용삼, "Assessment of Genetic Variation among Commercial Tomato (Solanum lycopersicum L.) Varieties Using SSR Markers and Morphological Characteristics", Genes & Genomics, 31(1), pp.1~10 (2009), 한국유전학회
- 김혁준, "박과작물의 유연관계 분석을 통한 수박 EST-SSR 마커의 종간 적용성 검정", 원예과학기술지, 33(1), pp.93~105 (2015), 한국원예학회
- 김나휘, "The CmACS-7 Gene Provides Sequence Variation for Development of DNA Markers Associated with Monoecious Sex Expression in Melon (Cucumis melo L.)", Horticulture, Environment, and Biotechnology, 56(4), pp.535~545 (2015), 한국원예학회[DOI]
- Mantel N, "The detection of disease clustering and a generalized repression approach", Cancer Res, 27, pp.209~220 (1967)
- Nei M, "Mathematical model for studying genetic variation in terms of restriction endonucleases", Proc. Natl. Acad. Sci U.S.A, 76, pp.5269~5273 (1979)
- Pitrat M, "Some comments on infraspecific classification of cultivars of melon", Acta. Hort, 510, pp.29~36 (2000)
- Rohlf F, "NTSYSpc: Numerical taxonomy system, version 2.1" (2002), Exeter Publishing
- Sneath PHA, "Numerical taxonomy: The principles and practice of numerical classification" (1973), W. H. Freeman
- Schaefer H, "Gourds afloat: A dated phylogeny reveals an Asian origin of the gourd family (Cucurbitaceae) and numerous oversea dispersal events", Proc. Biol. Sci, 276, pp.843~851 (2009)
- 서동휘, "국내 수집 감 품종을 이용한 EST-SSR marker 개발과 유전적 다양성 분석", 한국자원식물학회지, 26(4), pp.491~502 (2013), 한국자원식물학회[DOI]
- 이승인, "참외·멜론의 품종 분류 및 명명에 관한 최근 동향과 분석", 원예과학기술지, 22(4), pp.515~522 (2004), 한국원예학회