Diversity based on Molecular Markers and Agronomic traits and Interspecific Crosses among Cucurbita spp.
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We have analyzed the genetic diversity of 45 squash genotypes in Cucurbita maxima, C. moschata, C. pepo and uniden-tified Cucurbita spp. (noodle-squash) with molecular markers. The 45 squash accesions showed molecular variations of 87.5% inRAPD and 74.4% in AFLP, respectively, which allowed to group them into 3 clusters at the genetic similarity of 54%. Noodle-squashlines were grouped into the same cluster with C. pepo at 89% similarity level. Genetic similarities within individual sub-groups were97% in noodle-squashes, 96% in C. pepo, 98% in C. moschata, and 98% in C. maxima, respectively. Korean, Japanese, and Americannoodle-squashes were separately grouped at the similarity of 97%. Since there were no distinct molecular markers to distinguish thenoodle-squashes and C. pepo in our study, we suggest that the noodle-squash could be one of the different ecotypes of C. pepo. In theagronomic traits of the noodle-squashes and three Cucurbita spp., the noodle-squash showed closer to the C. pepo than the other twoCucurbita species. In inter-specific crosses with the noodle-squash, the noodle-squash/C. pepo showed higher fruit and seed settingthan the other cros combinations. These results will be helpful to the studies of squash breding programs.
Abstract
We have analyzed the genetic diversity of 45 squash genotypes in Cucurbita maxima, C. moschata, C. pepo and uniden-tified Cucurbita spp. (noodle-squash) with molecular markers. The 45 squash accesions showed molecular variations of 87.5% inRAPD and 74.4% in AFLP, respectively, which allowed to group them into 3 clusters at the genetic similarity of 54%. Noodle-squashlines were grouped into the same cluster with C. pepo at 89% similarity level. Genetic similarities within individual sub-groups were97% in noodle-squashes, 96% in C. pepo, 98% in C. moschata, and 98% in C. maxima, respectively. Korean, Japanese, and Americannoodle-squashes were separately grouped at the similarity of 97%. Since there were no distinct molecular markers to distinguish thenoodle-squashes and C. pepo in our study, we suggest that the noodle-squash could be one of the different ecotypes of C. pepo. In theagronomic traits of the noodle-squashes and three Cucurbita spp., the noodle-squash showed closer to the C. pepo than the other twoCucurbita species. In inter-specific crosses with the noodle-squash, the noodle-squash/C. pepo showed higher fruit and seed settingthan the other cros combinations. These results will be helpful to the studies of squash breding programs.