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Neem 에탄올추출물과 분획물의 항산화, 항균 및 항염증 효과 비교

Comparison of Antioxidant, Antimicrobial, and Anti-inflammatory Effects of Ethanol Extract from Neem(Azadirachta indica) and Its Fractions

김미경
한국인체미용예술학회 (The Korean Society of Beauty and Art)|2021.03|한국인체미용예술학회지 (The Korean Society Of Beauty And Art)|제22권 제1호|pp. 61-74|DOI http://doi.org/10.18693/jksba.2021.22.1.61

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키워드

Antioxidant항균항염증항산화Anti-inflammatory총 폴리페놀Total Polyphenolic Contents님 분획물AntimicrobiaNeem Fraction
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초록

In this study, the antioxidant, antimicrobial, and anti-inflammatory activities of a 70% ethanol extract of neem (Azadirachta indica) leafves and their fractions were evaluated to confirm the applicability as a functional cosmetic material. It was found that the total polyphenol content was higher in butanol (181.77±3.25 mg/g) and ethyl acetate fraction (180.27±1.53 mg/g) as compared to ethanol extract (139.16±1.04 mg/g). The electron donating ability and ABTS radical scavenging ability increased in a concentration-dependent manner in all samples. The IC50 value of electron donating ability was lower (357.6-375.1 ㎍/㎖) in ethyl acetate and butanol fractions compared to the than in ethanol extract (384.5 ㎍/㎖), and the ABTS radical scavenging activity was higher in the butanol fraction (66.8%) than the ethanol extract (64.3%) at the concentration of 1,000 ㎍/㎖. The antimicrobial activity of S. epidermidis and S. aureus were high in the ethyl acetate fraction, 8.5-11.5 mm and 8.5-15.0 mm, respectively, and the antibacterial activity against E. coli was high in the butanol fraction (8.5-14.0 mm). However, the antimicrobial activity against P. acne did not show a significant difference between the extract and the fraction. Cytotoxicity for RAW 264.7 cells was not observed up to the concentration of 100 ㎍/㎖ in both the extracts or fractions. The inhibition rate of NO production induced by LPS stimulation was highest in the ethyl acetate fraction (84.3%) than in the LPS alone treatment group, and about 2.3 times that of the ethanol extract (36.9%). Therefore, it was confirmed that the ethyl acetate fraction of neem leaves had a significant effect on the total polyphenol content, antioxidant, antibacterial activity, and inhibition of NO. In the future, it is considered necessary to study the anti-inflammatory mechanism of the ethyl acetate fraction.

Abstract

In this study, the antioxidant, antimicrobial, and anti-inflammatory activities of a 70% ethanol extract of neem (Azadirachta indica) leafves and their fractions were evaluated to confirm the applicability as a functional cosmetic material. It was found that the total polyphenol content was higher in butanol (181.77±3.25 mg/g) and ethyl acetate fraction (180.27±1.53 mg/g) as compared to ethanol extract (139.16±1.04 mg/g). The electron donating ability and ABTS radical scavenging ability increased in a concentration-dependent manner in all samples. The IC50 value of electron donating ability was lower (357.6-375.1 ㎍/㎖) in ethyl acetate and butanol fractions compared to the than in ethanol extract (384.5 ㎍/㎖), and the ABTS radical scavenging activity was higher in the butanol fraction (66.8%) than the ethanol extract (64.3%) at the concentration of 1,000 ㎍/㎖. The antimicrobial activity of S. epidermidis and S. aureus were high in the ethyl acetate fraction, 8.5-11.5 mm and 8.5-15.0 mm, respectively, and the antibacterial activity against E. coli was high in the butanol fraction (8.5-14.0 mm). However, the antimicrobial activity against P. acne did not show a significant difference between the extract and the fraction. Cytotoxicity for RAW 264.7 cells was not observed up to the concentration of 100 ㎍/㎖ in both the extracts or fractions. The inhibition rate of NO production induced by LPS stimulation was highest in the ethyl acetate fraction (84.3%) than in the LPS alone treatment group, and about 2.3 times that of the ethanol extract (36.9%). Therefore, it was confirmed that the ethyl acetate fraction of neem leaves had a significant effect on the total polyphenol content, antioxidant, antibacterial activity, and inhibition of NO. In the future, it is considered necessary to study the anti-inflammatory mechanism of the ethyl acetate fraction.

참고문헌 (38)

  1. 박보람, "나고야의정서와 관련한 식품 의약품 및 화장품 분야에서의 대응방안", 한국에프디시법제학회지, 7, pp.69~81 (2012)
  2. 서수정, "맥문동(Liriope platyphylla) 열매 추출물의 항노화 효과 및 항산화 활성", 대한미용학회지, 9(2), pp.105~114 (2013), 대한미용학회[DOI]
  3. A. Moure, "Natural antioxidants from residual sources", Food Chem, 72(2), pp.145~171 (2001)
  4. A. Akerels., "Summary of WHO guidelines for the assessment of herbal medicines herbal gram", Nirali Prakashan, 22, pp.13~28 (1993)
  5. R. Tiwari, "Neem(Azadirachta indica)and its potential for safeguarding health of animals and humans : A review", J Biol Sc, 14(2), pp.110~123 (2014)
  6. A. Mohammad, "A review on spermicidal activities of Azadirachta indica", Journal of Pharmacognosy and Phytochemistry, 1(5), pp.61~79 (2013)
  7. Z. Y. Ping, "Global distribution and introduction cultivation state of Azadirachia indica", Forest Inventory and Planning, 792, pp.34~42 (2002)
  8. P. Prieto, "Activity of a standardized neem(Azadirachia indica)seed extract on the rodent malaria parsite plasmodium berghei", Analytical Biochemistry, 269, pp.337~341 (1999)
  9. H. Eshrat, "Using traditional Indian anti-diabetic plant Azadirachia indica", Indian Journal of Clinical Biochemistry, 17, pp.115~123 (2002)
  10. N. Morisaki, "Mechanism of angiogenic effects of Saponin from ginseng dixruber in human umbilical vein endothelial cells", Br J Pharmacol, 11(7), pp.1188~1193 (1995)
  11. R. R. Chattopadhyay, "Possible mechanism of anti-inflammatory activity of Azadirachta indica leaf extract", Indian J Pharm, 25(2), pp.99~100 (1993)
  12. M. A. Hossain, "Identification and characterization of chemical compounds in different crude extracts from leaves of Omani neem", Journal of Taibah University for Science, 7, pp.181~188 (2013)
  13. S. Johnson, "Comparison of chromatographic systems for triterpenoids from neem(Azadirachta indica)seeds", J Chromatogr A., 761, pp.53~63 (1997)
  14. J. W. Lee, "Investigation on the insecticidal limonoid content of commercial biopesticide and neem extract using solid phase extraction", J Agric Chem Environ, 2(4), pp.81~85 (2013)
  15. P. Caboni, "Fate of azadiracthin A and related azadira-chtoids on tomatoes after greenhouse treatment", J Environ Sci Health B, 44, pp.598~605 (2009)
  16. 김미경, "추출방법에 따른 Neem의 항산화, 항균 활성 및 항염 효과 비교", 한국인체미용예술학회지, 20(3), pp.265~278 (2019), 한국인체미용예술학회[DOI]
  17. 현진아, "님트리(Azadirachta indica)잎 에탄올추출물의 약리활성 및 안티폴루션 효능 검증", Food Science and Preservation, 27(3), pp.393~406 (2020), 한국식품저장유통학회
  18. V. L. Sigleton, "Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents", Am J Enol Viticult, 16, pp.144~158 (1965)
  19. 모정희, "화장품 소재로서 더덕과 발효더덕 추출물의 피부관련생리활성 비교탐색", 한국인체미용예술학회지, 14(4), pp.49~60 (2013), 한국인체미용예술학회
  20. N. Fellegrini, "Screening of dietary carotenoids and carotenoid-rich fruit extracts for antioxidant activities applying 2, 2'-azinobis(3-ethylenebenzothazoline-6-sulfonic acid radical cation decolorization assay", Method Enzymol, 299, pp.379~389 (1999)
  21. R. S. Farag, "Antimicrobial activity of some Egyptian spice essential oils", J Food Prot, 52(9), pp.665~667 (1989)
  22. C. A. Begona, "Fermented wheat germ extract inhibits glycolysis pentose cycle enzymes and induces apoptosis through poly(ADP-ribose)polymerase activation in Jurkat T cell leukemia tumor cells", J Biol Chem, 277(4), pp.46408~46414 (2002)
  23. nitrate in biological fluids, "Anal Biochem", 1982, 131~138
  24. 김정훈, "울릉 섬바디 추출물의 라디칼 소거 및 α-glucosidase 저해 활성", Food Science and Preservation, 26(2), pp.246~252 (2019), 한국식품저장유통학회
  25. 송종호, "어성초(Houttuynia cordata) 뿌리에서 추출한 순차분획물의 항균활성", 한국식품영양과학회지, 32(7), pp.1053~1058 (2003), 한국식품영양과학회
  26. K. Tawaha, "Antioxidant activity and total phenolic content of selected Jordanian plant species", Food Chem, 104, pp.1372~1378 (2007)
  27. I. Gulcin, "Antiradical and antioxidant activity of total anthocyanins from Perilla pankinensis decne", J Ethmopharmacol, 101, pp.287~293 (2005)
  28. 전상민, "참취 추출물과 용매분획물의 항산화 활성", 한국식품영양과학회지, 41(9), pp.1197~1204 (2012), 한국식품영양과학회
  29. 유미희, "로즈마리 추출물 및 분획물의 항균·항산화 활성", 생명과학회지, 21(3), pp.375~384 (2011), 한국생명과학회
  30. D. B. R. Van, "Applicability of an improved Trolox equivalent antioxidant capacity(TEAC)assay for evaluation of antioxidant capacity measurements of mixtures", Food Chem, 66, pp.511~517 (1999)
  31. J. M. Awika, "Screening methods to measure antioxidant activity of sorghum(Sorghum bicolor)and sorghum products", J Agric Food Chem, 51, pp.6657~6662 (2003)
  32. 최용민, "국내 시판되는 일부 다류 제품의 항산화 효과", 한국식품영양과학회지, 32(5), pp.723~727 (2003), 한국식품영양과학회
  33. 김대용, "브로콜리 추출물의 향장활성 효과", 한국인체미용예술학회지, 17(1), pp.29~39 (2016), 한국인체미용예술학회
  34. 정숙현, "엽차용 녹차 추출물 및 분획물의 항균효과", 한국식품영양과학회지, 37(11), pp.1382~1388 (2008), 한국식품영양과학회
  35. 이윤주, "지모 뿌리 추출물과 분획물의 항균활성과 항산화 활성 및 세포보호 연구", 한국미생물·생명공학회지, 46(4), pp.360~371 (2018), 한국미생물·생명공학회
  36. N. McCartney-Francis, "Suppression of arthritis by an inhibitor of nitric oxide synthase", J Exp Med, 178, pp.749~754 (1993)
  37. A. Weisz, "Regulation of the mouse inducible-type nitric oxide synthase gene promoter by interferon-gamma, bacterial lipopolysaccharide and NG-monomethyl-L-arginine", Biochem J, 316, pp.209~215 (1996)
  38. 김영선, "카카오(Theobroma cacao L.) 용매 분획물의 항산화 및 항염증 효과", 생명과학회지, 22(10), pp.1330~1338 (2012), 한국생명과학회[DOI]

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