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산벚나무 가지 추출물 및 용매 분획물의 Nitric Oxide 생성 억제 효과와 여드름 원인균에 대한 항균활성

Antibacterial and Nitric Oxide Production Inhibitory Activities of Prunus sargentii Branches Extract and Its Fractions against Pathogens of Acne

김선민|표병식|양선아
한국약용작물학회|2016.04|한국약용작물학회지 (KOREAN JOURNAL OF MEDICINAL CROP SCIENCE)|제24권 제2호|pp. 129-135|DOI http://dx.doi.org/10.7783/KJMCS.2016.24.2.129

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키워드

AcnePrunus sargentiiAnti-InflammatoryAntibacterial ActivityAntioxidative Activity

초록

Background: In this study, we investigated the antibacterial and nitric oxide (NO) production inhibitory activities of 75% ethanol extract of Prunus sargentii branches and its fractions against acne pathogens. Methods and Results: The antibacterial activity against acne causing pathogens was determined using the disc diffusion assay. The ethyl acetate fraction showed higher activities against Propionibacterium acnes, Staphylococcus aureus and Staphylococcus epidermidis than those shown by other fractions. In the DPPH radical and NO scavenging assays, the butanol fraction showed strong DPPH radical and NO scavenging abilities. These activities were related to the total polyphenol and flavonoid contents of butanol fraction. On the other hand, the chloroform and ethyl acetate fractions exhibited the highest NO production inhibitory activity in Lipopolysaccharide (LPS)-stimulated Raw 264.7 cells compared to those exhibited by other fractions. Conclusions: The extract and its ethyl acetate fraction from the branches of P. sargentii exhibited antibacterial activity and could be used as functional materials in antimicrobial related fields. Moreover, the chloroform and ethyl acetate fractions are potential antiinflammatory agents and butanol fraction acts as an effective radical scavenger.

Abstract

Background: In this study, we investigated the antibacterial and nitric oxide (NO) production inhibitory activities of 75% ethanol extract of Prunus sargentii branches and its fractions against acne pathogens. Methods and Results: The antibacterial activity against acne causing pathogens was determined using the disc diffusion assay. The ethyl acetate fraction showed higher activities against Propionibacterium acnes, Staphylococcus aureus and Staphylococcus epidermidis than those shown by other fractions. In the DPPH radical and NO scavenging assays, the butanol fraction showed strong DPPH radical and NO scavenging abilities. These activities were related to the total polyphenol and flavonoid contents of butanol fraction. On the other hand, the chloroform and ethyl acetate fractions exhibited the highest NO production inhibitory activity in Lipopolysaccharide (LPS)-stimulated Raw 264.7 cells compared to those exhibited by other fractions. Conclusions: The extract and its ethyl acetate fraction from the branches of P. sargentii exhibited antibacterial activity and could be used as functional materials in antimicrobial related fields. Moreover, the chloroform and ethyl acetate fractions are potential antiinflammatory agents and butanol fraction acts as an effective radical scavenger.

참고문헌 (28)

  1. Anagnostopoulou MA, "Radical scavenging activity of various extracts and fractions of sweet orange peel(Citrus sinensis)", Food Chemistry, 94, pp.19~25 (2006)
  2. Blois MS, "Antioxidant determinations by the use of a stable free radical", Nature, 181, pp.1199~1200 (1958)
  3. 최용민, "국내 시판되는 일부 다류 제품의 항산화 효과", 한국식품영양과학회지, 32(5), pp.723~727 (2003), 한국식품영양과학회
  4. Cunliffe WJ, "Comedone formation: Etiology, clinical presentation and treatment", Clinics in Dermatology, 22, pp.367~374 (2004)
  5. Dewick PM, "Medicinal natural products", pp.149~151 (2002), John Wiley and Sons
  6. Ding AH, "Release of reactive nitrogen intermediates and reactive oxygen intermediates from mouse peritoneal macrophages: Comparison of activating cytokines and evidence for independent production", The Journal of Immunology, 141, pp.2407~2412 (1988)
  7. Folin O, "On phosphotungstic-phosphomolybdic compounds as color reagents", Journal of Biological Chemistry, 12, pp.239~243 (1912)
  8. Kang GJ, "Inhibitory effect of organic extracts from Prunus yedoensis Matsum barks on the atopic dermatitis-like inflammation", Master, pp.2007
  9. 기호균, "여드름 환자에서 세균배양과 항생제 감수성에 관한 연구", 대한피부과학회지, 43(7), pp.871~875 (2005), 대한피부과학회
  10. 김현주, "벚나무의 꽃과 유엽의 제다 방법에 따른 차의 무기성분 및 관능평가에 미치는 영향", 한국인간식물환경학회지, 9(4) (2006), 한국인간식물환경학회
  11. 김상숙, "부지화 미숙과 에센셜 오일의 항산화 및 항균 활성 효과", 한국약용작물학회지, 21(6), pp.493~497 (2013), 한국약용작물학회[DOI]
  12. Kim YH, "The characterization of anthocyanin pigments prepared from cherry(Prunus serrulata L. var. spontanea Max. Wils.) for the potential sources of red colorant", Journal of the Korean Society for Applied Biological Chemistry, 42, pp.134~169 (1999)
  13. Lee ES, "Inhibition of nitric oxide and tumor necrosis factor-α(TNF-α) production by propenone compound through blockade of nuclear factor(NF)-κB activation in cultured murine macrophage", Biological and Pharmaceutical Bulletin, 27, pp.617~620 (2004)
  14. Lee SS, "Studies on biological activity of wood extractives(VII): Antimicrobial and antioxidation activities of extractives from the heartwood of Prunus sargentii", Journal of the Korean Wood Science and Technology, 29, pp.140~145 (2001)
  15. 이화, "베리류 에탄올 추출물의 항산화 활성", 한국약용작물학회지, 23(1), pp.49~56 (2015), 한국약용작물학회[DOI]
  16. Marcocci L, "The nitric oxide-scavenging properties of Ginkgo biloba extract EGb 761", Biochemical and Biophysical Research Communications, 201, pp.748~755 (1994)
  17. Moreno MIN, "Comparison of the free radical-scavenging activity of propolis from several region of Argentina", Journal of Enthropharmacology, 71, pp.109~114 (2000)
  18. 박은영, "황촉규 추출물의 Nitric Oxide 생성 저해활성", 약 학 회 지, 52(4), pp.259~263 (2008), 대한약학회
  19. 박주아, "소리쟁이(Rumex crispus L.) 뿌리 추출물의 피부상재균에 대한 항균활성과 항염증 효과", 아시안뷰티화장품학술지, 9(2), pp.9~16 (2011), 한국피부과학연구원
  20. 박정미, "산벚나무 수피추출물의 생리활성 및 페놀성 화합물 분리", 한국약용작물학회지, 16(3), pp.173~182 (2008), 한국약용작물학회
  21. 박윤점, "벚나무 꽃과 유엽 추출물의 항균, 항산화 및 항염증 효과", 한국인간식물환경학회지, 10(1), pp.43~49 (2007), 한국인간식물환경학회
  22. Raimer SS, "Managing pediatric atopic dermatitis", Clinical Pediatric, 39, pp.1~14 (2000)
  23. 신경민, "Amygdalin의 Murine Macrophage Raw 264.7 세포에서 in vitro 항염효과", 생약학회지, 34(3), pp.223~227 (2003), 한국생약학회
  24. Shin MK, "Clinical traditional herbalogy", pp.399~400 (2006), Younglimsa
  25. Sivaranjani N, "Role of reactive oxygen species and antioxidants in atopic dermatitis", Journal of Clinical and Diagnostic Research, 7, pp.2683~2685 (2013)
  26. 송종호, "생열귀나무 잎 추출물과 분획물의 항산화 및 NO생성 억제 활성", 한국약용작물학회지, 23(1), pp.20~26 (2015), 한국약용작물학회[DOI]
  27. Velioglu YS, "Antioxidant activity and total phenolics in selected fruits, vegetables, and grain products", Journal of Agricultural and Food Chemistry, 46, pp.4113~4117 (1998)
  28. 양선아, "산벚나무 잎 추출물 및 분획물의 항균활성과 Nitric Oxide 생성억제 활성", 한국약용작물학회지, 20(5), pp.308~314 (2012), 한국약용작물학회

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