피부 항노화제, 라파콘에 관한 연구
Study of lapachone, skin anti-aging agent
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The action mechanism of β-lapachone on fibrillogenesis in human dermal fibroblasts was studied to develop a potential skin anti-aging agent. In a prior study, we found that β-lapachone stimulated type I collagen expression in human dermal fibroblasts. In cultured human dermal fibroblasts, both Smad 2 and Smad 3 (Smad 2/3) were phosphorylated by β-lapachone treatment, suggesting that β-lapachone stimulates type I collagen synthesis via the transforming growth factor-β receptor I kinase-dependent pathway. Secreted transforming growth factor-β I levels were not elevated after β-lapachone treatment, suggesting that the molecular mechanism of β-lapachone for the upregulation of collagen synthesis is due to the extracellular regulation of availability and activities of TGF-β. Our findings provide new insights into the role of β-lapachone in collagen synthesis in human dermal fibroblasts. These findings could be used for developing novel therapeutic strategies for treating aging skin.
Abstract
The action mechanism of β-lapachone on fibrillogenesis in human dermal fibroblasts was studied to develop a potential skin anti-aging agent. In a prior study, we found that β-lapachone stimulated type I collagen expression in human dermal fibroblasts. In cultured human dermal fibroblasts, both Smad 2 and Smad 3 (Smad 2/3) were phosphorylated by β-lapachone treatment, suggesting that β-lapachone stimulates type I collagen synthesis via the transforming growth factor-β receptor I kinase-dependent pathway. Secreted transforming growth factor-β I levels were not elevated after β-lapachone treatment, suggesting that the molecular mechanism of β-lapachone for the upregulation of collagen synthesis is due to the extracellular regulation of availability and activities of TGF-β. Our findings provide new insights into the role of β-lapachone in collagen synthesis in human dermal fibroblasts. These findings could be used for developing novel therapeutic strategies for treating aging skin.
참고문헌 (22)
- de Almeida ER, "Anti-inflammatory action of lapachol", J. Ethnopharmacol., 29, pp.239~241 (1990)
- Ueda S, "Production of anti-tumour-promoting furanonaphthoquinones in Tabebuia avellanedae cell cultures", Phytochemistry, 36, pp.323~241 (1990)
- Lai CC, "β-lapachone induced cell death in human hepatoma (HepA2) cells", Histol. Histopathol, 13, pp.323~325 (1994)
- Chau YP, "Involvement of hydrogen peroxide in topoisomerase inhibitor β-lapachone induced apoptosis and differentiation in human leukemia cells", Free Radic. Biol Med., 24, pp.660~670 (1998)
- Fu SC, "β-lapachone accelerates the recovery of burn-wound skin", Histol. Histopathol., 26(7), pp.905~914 (2011)
- Kung HN, "in vitro and in vivo wound healing-promoting activities of beta-lapachone", Am J Physiol Cell Physiol., 295(4), pp.C931~C943 (2008)
- Schultz GS, "Interactins between extracellular matrix and growth factors in wound healing", Wound Repair Regen., 17, pp.153~162 (2009)
- Vuorio E, "The family of collagen genes", Annu. Rev. Biochem., 59, pp.837~872 (1990)
- Choi MS, "Increase of collagen synthesis by obovatol through stimulation of the TGF-beta signaling and inhibition of matrix metalloproteinase in UVB-irradiated human fibroblast", J. Dermatol. Sci., 46, pp.127~137 (2007)
- Quan T, "Solar ultraviolet irradiation reduces collagen in photoaged human skin by blocking transforming growth factor-β type Ⅱ receptor", Smad signaling, 2004, pp.165, 741~751
- Cohen MP, "The renal TGF-β system in the db", db mouse model of diabetic nephropathy, 1998, pp.6, 226~233
- Isono M, "Stimulation of TGF-beta type Ⅱreceptor by high glucolse in mouse mesangial cells and in diabetic kidney", Am. J. Physiol. Renal Physiol., 278, pp.F830~F838 (2000)
- Wolf G, "Angiotensin Ⅱ stimulates expression of transforming growth factor beta receptor type Ⅱ in cultured mouse proximal tubular cells", J. Mol. Med., 77, pp.556~564 (1999)
- Cheng J, "Transforming growth factor-beta signal transduction and progressive renal disease", Minireview. Exp. Biol. Med, 227, pp.943~956 (2002)
- Mehra A, "TGF-beta and the Smad signal transduction pathway", Biochem. Cell Biol., 80, pp.605~622 (2002)
- Cutroneo KR., "How is type Ⅰ procollagen synthesis regulated at the gene level during tissue fibrosis", J. Cell. Biochem, 90, pp.1~5 (2003)
- Lutz M, "Integration of the TGF-β pathway in to the cellular signalling network", Cell. Signal., 14, pp.977~988 (2002)
- Leask A, "TGF-beta signaling and the fibrotic response", FASEB J ., 18(7), pp.816~827 (2004)
- Xin C, "Sphingosine 1-phosphate cross-activates the Smad signaling cascade and mimics transforming growth factor-beta-induced cell responses", J. Biol. Chem., 279, pp.35255~35262 (2004)
- Chen SJ, "Stimulation of type Ⅰ collagen transcription in human skin fibroblasts by TGF-beta: involvement of Smad 3", J. Invest. Dermatol., pp.11249~11257 (1999)
- Lee MK, "TGF-beta activates E가 MAP kinase signalling through direct phosphorylation of ShcA", EMBO J ., 26, pp.3957~3967 (2007)
- Chang C, "Agonists and antagonists of the TGF-β family ligands. The TGF-β family", pp.203~257 (2008), Cold Spring Harbor