Comparative evaluation of Emblica officinalis as an etchant and an MMP inhibitor with orthophosphoric acid and chlorhexidine on the microshear bond strength of composite resin: an ex vivo study
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Objectives: This study aimed to evaluate Emblica officinalis (Indian gooseberry or amla) as an acid etchant and matrix metalloproteinase (MMP) inhibitor, and to compare its effect on the microshear bond strength of composite resin with orthophosphoric acid (OPA) and 2% chlorhexidine (CHX) as an acid etchant and MMP inhibitor, respectively. Materials and Methods: The etching effect and MMP-inhibiting action of amla on dentin samples were confirmed by scanning electron microscopy (SEM) and gelatin zymography, respectively. Dentinal slabs (3 mm thick) from 80 extracted human molars were divided into 10 and 20 samples to form 2 control groups and 3 experimental groups. Groups 1, 2, and 4 were etched with OPA and groups 3 and 5 with amla juice. An MMP inhibitor was then applied: CHX for group 2 and amla extract for groups 4 and 5. Groups 1 and 3 received no MMP inhibitor. All specimens received a standardized bonding protocol and composite resin build-up, and were subjected to microshear bond strength testing. The force at which the fracture occurred was recorded and statistically analyzed. Results: Amla juice had a similar etching effect as a self-etch adhesive in SEM and 100% amla extract was found to inhibit MMP-9 by gelatin zymography. The microshear bond strength values of amla were lower than those obtained for OPA and CHX, but the difference was not statistically significant. Conclusions: Amla has a promising role as an acid etchant and MMP inhibitor, but further studies are necessary to substantiate its efficacy.
Abstract
Objectives: This study aimed to evaluate Emblica officinalis (Indian gooseberry or amla) as an acid etchant and matrix metalloproteinase (MMP) inhibitor, and to compare its effect on the microshear bond strength of composite resin with orthophosphoric acid (OPA) and 2% chlorhexidine (CHX) as an acid etchant and MMP inhibitor, respectively. Materials and Methods: The etching effect and MMP-inhibiting action of amla on dentin samples were confirmed by scanning electron microscopy (SEM) and gelatin zymography, respectively. Dentinal slabs (3 mm thick) from 80 extracted human molars were divided into 10 and 20 samples to form 2 control groups and 3 experimental groups. Groups 1, 2, and 4 were etched with OPA and groups 3 and 5 with amla juice. An MMP inhibitor was then applied: CHX for group 2 and amla extract for groups 4 and 5. Groups 1 and 3 received no MMP inhibitor. All specimens received a standardized bonding protocol and composite resin build-up, and were subjected to microshear bond strength testing. The force at which the fracture occurred was recorded and statistically analyzed. Results: Amla juice had a similar etching effect as a self-etch adhesive in SEM and 100% amla extract was found to inhibit MMP-9 by gelatin zymography. The microshear bond strength values of amla were lower than those obtained for OPA and CHX, but the difference was not statistically significant. Conclusions: Amla has a promising role as an acid etchant and MMP inhibitor, but further studies are necessary to substantiate its efficacy.
참고문헌 (24)
- Mazzoni A, "Substantivity of carbodiimide inhibition on dentinal enzyme activity over time", J Dent Res, 96, pp.902~908 (2017)
- Mazzoni A, "MMP activity in the hybrid layer detected with in situ zymography", J Dent Res, 91, pp.467~472 (2012)
- Heshmat H, "Effect of chlorhexidine on micro-shear bond strength of two adhesive systems after aging", J Islam Dent Assoc Iran, 30, pp.9~14 (2018)
- Hass V, "Collagen cross-linkers on dentin bonding: Stability of the adhesive interfaces, degree of conversion of the adhesive, cytotoxicity and in situ MMP inhibition", Dent Mater, 32, pp.732~741 (2016)
- Pashley DH, "Collagen degradation by host-derived enzymes during aging", J Dent Res, 83, pp.216~221 (2004)
- Fawzy AS, "Proanthocyanidins-Loaded Nanoparticles Enhance Dentin Degradation Resistance", J Dent Res, 96, pp.780~789 (2017)
- de Macedo FA, "Dentin bonding and physicochemical properties of adhesives incorporated with epigallocatechin-3-gallate", Odontology, 107, pp.23~28 (2019)
- Carrilho MR, "In vivo preservation of the hybrid layer by chlorhexidine", J Dent Res, 86, pp.529~533 (2007)
- Breschi L, "Use of a specific MMP-inhibitor (galardin) for preservation of hybrid layer", Dent Mater, 26, pp.571~578 (2010)
- Jain PK, "Pharmacognostic comparison of bacopa monnieri, cyperus rotundus and emblica officinalis", Innov J Ayruvedic Sci, 4, pp.16~26 (2016)
- Fujii T, "Amla (Emblica officinalis Gaertn.) extract promotes procollagen production and inhibits matrix metalloproteinase-1 in human skin fibroblasts", J Ethnopharmacol, 119, pp.53~57 (2008)
- Abraham S, "Evaluation of the inhibitory effect of triphala on PMN-type matrix metalloproteinase (MMP-9)", J Periodontol, 76, pp.497~502 (2005)
- Breschi L, "Dental adhesion review: aging and stability of the bonded interface", Dent Mater, 24, pp.90~101 (2008)
- De Munck J, "Enzymatic degradation of adhesive-dentin interfaces produced by mild self-etch adhesives", Eur J Oral Sci, 118, pp.494~501 (2010)
- Green B, "Grape seed proanthocyanidins increase collagen biodegradation resistance in the dentin", adhesive interface when included in an adhesive, 2010, pp.38, 908~915
- Susin AH, "Comparative scanning electron microscopic study of the effect of different dental conditioners on dentin micromorphology", J Appl Oral Sci, 16, pp.100~105 (2008)
- Mazzoni A, "Zymographic analysis and characterization of MMP-2 and -9 forms in human sound dentin", J Dent Res, 86, pp.436~440 (2007)
- Gupta P, "Natural products as inhibitors of matrix metalloproteinases", Nat Prod Chem Res, 4, pp.e114~ (2016)
- Breschi L, "Chlorhexidine stabilizes the adhesive interface: a 2-year in vitro study", Dent Mater, 26, pp.320~325 (2010)
- Breschi L, "Influence of chlorhexidine concentration on the durability of etch-and-rinse dentin bonds: a 12-month in vitro study", J Adhes Dent, 11, pp.191~198 (2009)
- 강현정, "Effect of different chlorhexidine application times on microtensile bond strength to dentin in Class I cavities", Restorative Dentistry and Endodontics, 37(1), pp.9~15 (2012), 대한치과보존학회
- Lapinska B, "Dentine surface morphology after chlorhexidine application-SEM study", Polymers (Basel), 10, pp.905~ (2018)
- Ahmed AA, "Microshear bond strength of universal adhesives to dentin used in total-etch and self-etch modes", Tanta Dent J, 15, pp.91~98 (2018)
- Souza M, "Evaluation of chlorhexidine substantivity on human dentin: a chemical analysis", J Endod, 38, pp.1249~1252 (2012)