신서란(Phormium tenax) 잎 조추출물 및 용매 분획물의 항염증 및 항아토피 효과
Anti-inflammatory and Anti-Atopic Effects of Crude Extracts and Solvent Fractions of Phormium tenax leaf
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초록
본 연구는 하라케케(Harakeke)로 불리는 신서란(Phormiumtenax)를 화장품 및 의약품산업의 기능성 소재로서의 이용 가능성을 확인하기 위하여 신서란 잎을 대상으로 70% 에탄올 추출물과 용매 분획물을 제조하여, 이것들의 항염증 및 항아토피의효과를 조사하였다. LPS로 유도된 RAW 264.7 세포에서 신서란에탄올 추출물과 용매 분획물의 항염증 효과를 조사한 결과,methylene chloride와 ethyl acetate 분획물에서 NO와 PGE2 생성 억제 활성이 가장 높게 나타났으며, 농도 의존적으로 NO와 PGE2 생성 억제 활성을 보였다. 또한, 이들 분획물에서는iNOS 및 COX-2 발현 억제 활성을 보였다. 신서란 잎 조추출물과 용매 분획물에 의한 NO, PGE2 생성 억제 활성이 NOS 및COX-2 발현 억제에 의한 것임을 제시한다. 더불어, hIFN-γ로자극된 HaCaT 세포에 용매 분획물을 처리하여 MDC 및 TRAC생성억제 효과를 조사한 바, methylene chloride 분획물은MDC 및 TATC의 생성을 각각 65%, 52% 생성억제 시켰으며,ethyl acetate 분획물은 MDC 및 TATC의 생성을 각각93%, 84%억제 효과를 보였다. 이상의 결과는 신서란 잎 조추출물과 용매분획물을 이용한 항염증 및 항아토피 효능을 갖는 유효성분 분리 및 활용화 연구에 중요한 기초자료가 될 것이며, 기능성 화장품, 의약외품 및 의약품 소재 개발에 적용 가능성이 높다고 사료된다.
Abstract
This study describes a preliminary evaluation of the anti-inflammatory activity and anti-atopic activity ofPhormium tenax leaf extracts. P. tenax leaf was extracted using 70% ethanol and then fractionated sequentially withn-hexane, methylene chloride, ethyl acetate, n-butanol. In order to effectively screen for anti-inflammatory agents, we firstinvestigated the inhibitory effects of P. tenax leaf crude extracts and solvent fractions on production of pro-inflammatoryfactors[nitric oxide(NO), prostaglandin E2(PGE2), inducible nitric oxide synthase(iNOS) and cyclooxygenase-2(COX-2)]and pro-inflammatory cytokines [tumor necrosis factor-α(TNF-α), interleukin-6(IL-6) and interleukin-1β(IL-1β)] inlipopolysaccharide(LPS)-stimulated RAW 264.7 cells. In addition, we also evaluated of their inhibitory effect on the atopicdermatitis-like inflammatory markers such as macrophage-derived chemokine(MDC) and thymus and activation-regulatedchemokine(TARC) in HaCaT cells. Among the five solvent fractions of P. tenax, methylene chloride and ethyl acetatefractions inhibited production of pro-inflammatory factors and pro-inflammatory cytokines in a dose dependent manner,respectively. These fractions were also showed inhibitory activity for MDC and TARC expression levels in IFN-γ-stimulated HaCaT cells, respectively. These results suggest that P. tenax have significantly effects of anti-inflammatoryactivity and anti-atopic activity that might be beneficial for the topical treatment of inflammatory skin disorders.
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