마치현(Portulaca oleracea L.) 추출물의 항염 및 항소양 작용: LPS 처리로 염증을 유도한 Raw264.7세포와 각질형성세포, NC/Nga 마우스, Hairless 마우스에 대한 결과
Anti-inflammatory and Anti-pruritic Effects of Portulaca oleracea L. Extract Using In Vitro and In Vivo Inflammation Model: LPS-treated Raw264.7 Cells, Keratinocytes, NC/Nga Mice and Hairless SKH-1 Mice
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Background: Portulaca oleracea L. is known to have detoxification and antibacterial effects. Inflammatory responses in LPS-treated Raw264.7 cells include the secretions of NO,PGE2 and numerous cytokines. Atopic dermatitis is a chronic inflammatory skin disease. This study was performed to identify the antiinflammatory or anti-pruritic effects of Portulaca oleracea L. extract in LPS-treated Raw264.7 cells and keratinocytes and the skin of NC/Nga mice with atopic dermatitis, as well as hairless mice with pruritus. Methods: After the treatment of different concentrations for Portulaca oleracea L. extract in LPS-treated Raw264.7 cells and keratinocytes, the levels of NO, PGE2 and proinflammatory cytokines were measured in the media. Skin tissues of all Nc/Nga mice were subjected to H&E staining and toluidine blue staining. An antipruritic effect was defined as the number of scratching behaviors of the hairless SKH-1 mice treated with compound 48/80. Results: LPS- and IFN-γ-treated Raw264.7 cells decreased NO and PGE2 productions compared to non-treated Raw264.7cells. H&E or toluidine blue staining revealed that the thicknesses of the epidermis and dermis were reduced in NC/Nga mice with atopic dermatitis treated with Portulaca oleracea L. extract. However, Itching behaviors in hairless mice treated with Portulaca oleracea L. extract were not decreased compared to non-treated hairless mice. Conclusion: The results of this study suggest that Portulaca oleracea L. extract could be a new therapeutic agent for atopic dermatitis.
Abstract
Background: Portulaca oleracea L. is known to have detoxification and antibacterial effects. Inflammatory responses in LPS-treated Raw264.7 cells include the secretions of NO,PGE2 and numerous cytokines. Atopic dermatitis is a chronic inflammatory skin disease. This study was performed to identify the antiinflammatory or anti-pruritic effects of Portulaca oleracea L. extract in LPS-treated Raw264.7 cells and keratinocytes and the skin of NC/Nga mice with atopic dermatitis, as well as hairless mice with pruritus. Methods: After the treatment of different concentrations for Portulaca oleracea L. extract in LPS-treated Raw264.7 cells and keratinocytes, the levels of NO, PGE2 and proinflammatory cytokines were measured in the media. Skin tissues of all Nc/Nga mice were subjected to H&E staining and toluidine blue staining. An antipruritic effect was defined as the number of scratching behaviors of the hairless SKH-1 mice treated with compound 48/80. Results: LPS- and IFN-γ-treated Raw264.7 cells decreased NO and PGE2 productions compared to non-treated Raw264.7cells. H&E or toluidine blue staining revealed that the thicknesses of the epidermis and dermis were reduced in NC/Nga mice with atopic dermatitis treated with Portulaca oleracea L. extract. However, Itching behaviors in hairless mice treated with Portulaca oleracea L. extract were not decreased compared to non-treated hairless mice. Conclusion: The results of this study suggest that Portulaca oleracea L. extract could be a new therapeutic agent for atopic dermatitis.
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