Production of Bioactive Compounds from Fungi Grown on Ginseng- Steaming Effluent
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We described production of bioactive compounds from fungi grown on Korean ginseng-steaming effluents (GSE) for develop high-value added nutraceuticals from Korean GSE. Hansenula anomala KCCM 11473,which grew well in Korean GSE had high RNA content, and its optimal autolysis conditions were established to produce 5’-ribonucleotides (13.9~28.5 mg/g of biomass) at 55oC and pH 5.0 for 24 h. 5’-Phosphodiesterase and adenyl deaminase were not effective in increasing the yield of 5’-ribinucleatides, but the yield of IMP increased significantly only after the addition of 1.0% adenyl deaminase. Saccharomyces cerevisiae showed the highest growth in the GSE medium. 267.1 mg of S. cerevisiae biomass was produced from 1 g of GSE solid and medicinal ginsenoside-Rg3 contents was determined with 0.033 mg. Mucor miehei KCTC 6011 produced approximately 120 mg of chitosan per g-dry mycelium in 84 h at 25oC when grown in the GSE (pH 8.0)supplemented with 0.5% yeast extract and 0.002% CuSO4. Chitosan produced by M. miehei KCTC 6011 have deacetylated approximately 56% and its viscosity and molecular weight of the chitosan were 80 cps and 1.07 x 103 kDa, respectively. The chitosan at 1.5 mg/ml inhibited 73.9% of the mycelium growth of Rhizotonia solani in 60 h.
Abstract
We described production of bioactive compounds from fungi grown on Korean ginseng-steaming effluents (GSE) for develop high-value added nutraceuticals from Korean GSE. Hansenula anomala KCCM 11473,which grew well in Korean GSE had high RNA content, and its optimal autolysis conditions were established to produce 5’-ribonucleotides (13.9~28.5 mg/g of biomass) at 55oC and pH 5.0 for 24 h. 5’-Phosphodiesterase and adenyl deaminase were not effective in increasing the yield of 5’-ribinucleatides, but the yield of IMP increased significantly only after the addition of 1.0% adenyl deaminase. Saccharomyces cerevisiae showed the highest growth in the GSE medium. 267.1 mg of S. cerevisiae biomass was produced from 1 g of GSE solid and medicinal ginsenoside-Rg3 contents was determined with 0.033 mg. Mucor miehei KCTC 6011 produced approximately 120 mg of chitosan per g-dry mycelium in 84 h at 25oC when grown in the GSE (pH 8.0)supplemented with 0.5% yeast extract and 0.002% CuSO4. Chitosan produced by M. miehei KCTC 6011 have deacetylated approximately 56% and its viscosity and molecular weight of the chitosan were 80 cps and 1.07 x 103 kDa, respectively. The chitosan at 1.5 mg/ml inhibited 73.9% of the mycelium growth of Rhizotonia solani in 60 h.
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