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Isolation of New Strain of Cordyceps militaris HB8 and Optimal Condition for Production of Adenosine and Cordycepin in Fruit Body

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Cordyceps has been used in traditional Chinese medicine more than 2000 year ago. In this study, the newCordyceps militaris was founded and isolated from O-dae mountain in Korea, and was identified its genetic characteristics. The newly isolation strain HB8 was most closet to Cordyceps militaris W141449 (99.82%), Cordyceps militarisJLCY-LI819 (99.82%) and Cordyceps militaris 4642 (99.81%), respectively. the genotypic result was show that train HB8was belonging to the Cordyceps militaris genus, therefore, Cordycep militaris HB8 proposed with accession numberMT835161. This study we find the optimal condition for production of cordycepin from Cordyceps militaris HB8 was 8 ㎎/g (200 g of pupa, 1 g of KH2PO4, 0.5 g of K2HPO4, 20 g of glucose, 1 g of MgSO4, 0.05 g of vitamin B1, and 1 ㎎ of NAAper liter; light condition 300-700 Lux and day/night was 14 h/10 h) and the optimum condition for the production ofadenosine was 2.6 ㎎/g (15 g of skim milk powder, 1 g of KH2PO4, 0.5 g of K2HPO4, 20 g of glucose, 1 g of MgSO4, 0.05g of vitamin B1, and 1 ㎎ of NAA per liter; light condition 300-700 Lux and day/night was 14 h/10 h).

Abstract

Cordyceps has been used in traditional Chinese medicine more than 2000 year ago. In this study, the newCordyceps militaris was founded and isolated from O-dae mountain in Korea, and was identified its genetic characteristics. The newly isolation strain HB8 was most closet to Cordyceps militaris W141449 (99.82%), Cordyceps militarisJLCY-LI819 (99.82%) and Cordyceps militaris 4642 (99.81%), respectively. the genotypic result was show that train HB8was belonging to the Cordyceps militaris genus, therefore, Cordycep militaris HB8 proposed with accession numberMT835161. This study we find the optimal condition for production of cordycepin from Cordyceps militaris HB8 was 8 ㎎/g (200 g of pupa, 1 g of KH2PO4, 0.5 g of K2HPO4, 20 g of glucose, 1 g of MgSO4, 0.05 g of vitamin B1, and 1 ㎎ of NAAper liter; light condition 300-700 Lux and day/night was 14 h/10 h) and the optimum condition for the production ofadenosine was 2.6 ㎎/g (15 g of skim milk powder, 1 g of KH2PO4, 0.5 g of K2HPO4, 20 g of glucose, 1 g of MgSO4, 0.05g of vitamin B1, and 1 ㎎ of NAA per liter; light condition 300-700 Lux and day/night was 14 h/10 h).

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