Human Immunodeficiency Virus Type Ⅰ에 대한 음나무 추출물의 억제활성
The Extracts of Kalopanax pictus Nakai. for Inhibitory Effects on HIV-1 and Its Essential Enzymes
2
조회
0
다운로드
0
인용
0
참고문헌
초록
Abstract
For the purpose of developing new anti-HIV agents from natural sources, the extracts of Kalopanax pictus were tested for their inhibitory effects on HIV-1 replication and its essential enzymes as the reverse transcriptase (RT), protease and α- glucosidase. In the assay of HIV-1-infected human T-cell line, water extracts of stem and leafstalk inhibited the HIV-1-induced cytopathic effects with IC (inhibitory concentration) of 25 and 50ug/ml, respectively. Moreover water extracts (100ug/ml) of stem and leafstalk showed strong activity of 80% and 90% on anti-HIV-1 RT using Enzyme Linked Oligonucleotide Sorbent Assay (ELOSA) method. In the HIV-1 protease inhibition assay, aqueous stem extract inhibited the activity of the enzyme to cleave an oligopeptide, resembling one of the cleavage sites in the viral polyprotein which can only be processed by HIV-1 protease with 58%, but no glucosidase inhibitory activities. We found out this result, for these samples it is possible that the inhibition of the viral replication in vitro is due to the inhibition at least one of RT and protease. It would be of great interest to identify the compounds which are responsible for this inhibition, since all therapeutically useful agent up to date are RT, PR and α-glucosidase inhibitors.
참고문헌 (22)
- "Natural product-based anti-HIV drug discovery and development facilitated by the NCI developmental therapeutics program Journal of Natural Products", pp.265~277 (2001)
- "Human Immunodeficiency Virus Type Ⅰ에대한 수종 식물 추출물의 억제활성 검색", pp.338~346 (1998)
- acridine marine alkaloids,
- "Inhibition of human immunodeficiency viral replication by tannins and related compounds", hata, pp.91~103 (1992)
- "Screening of various plant extracts used in ayurvedic medicine for inhibitory effects on human immunodeficiency virus type 1", and shimotoh, pp.~1
- "완역중약대사전" (1998), 도서출판정담
- "해동피로부터 Kalopanax - saponin B의 분리 및 함량분석", pp.277~284 (2002)
- "Flavonol glycosides from the leaves of Kalopanax pictum Korean Journal of Pharmacognosy"
- "음나무 수피의 화학적성분", pp.302~306 (2001)
- "해동피의 화학성분 및 생리활성", pp.122~129 (1995)
- "해동피추출물이 실험적으로 유발된 가토의 간장기능장애에 미치는 영향", pp.273~278 (1989)
- "Structural origins of high-affinity biotin binding to streptavidin", pp.85~88 (1989)
- "Anti-human immunodeficiency virus activity of som tropical medicinal plants"
- "Cellular distribution of HIV type Ⅰ nef protein Identification of domains in nef required for association with membrane and detergent-insoluble cellular matrix", pp.1003~1010 (1994), Kaminchik
- "Mechanism of DNA strand transfer reactions catalyzed by HIV-1 reversetranscriptase", pp.1112~1118 (1992)
- "Linker insertion mutagenesis of human immunodeficiency virus reversetranscriptase expressed in bacteria definition of the minimal polymerase domain", pp.3104~3108 (1989)
- "Inhibition of alpha-glucosidase I of the glycoprotein-processing enzymes by 6-O-butanoyl castanospermine and its consequences in human immunodeficiency virus-infected T cells", pp.1780~28 1787 (1994)
- "Inhibitory effect of tannins on reverse transcriptase from RNA tumor virus Journal of Natural Products", pp.614~621 (1985)
- "The V3 region of the envelope glycoprotein of human immunodeficiency virus type 1 binds sulfated polysaccharides and CD4-derived synthetic peptides Journal of Biological Chemistry", pp.6664~6671 (1992)
- "n-specific plant lectin from Urtica dioica are potent and selective inhibitors of human immunodeficiency virus and cytomegalovirus replication in vitro"
- "Triterpene derivatives that block entry of human immunodeficiency virus type 1 into cells", pp.3564~3568 (1994)
- "Antiviral agents targeted to interact with viral capsid proteins and a possible application to human immunodeficiency virus", pp.4625~4627 (1988)