능소화 잎 및 줄기 추출물의 Human Immunodeficiency Virus Type I 억제활성
Inhibitory Effects of Campsis grandiflora on HIV-1 reverse Transcriptase, HIV-1 Protease and α-glucosidase
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초록
적 요능소화 추출물의 HIV-1에 대한 항바이러스 효과를 복제 관련 효소에 대한 억제실험과 바이러스 복제억제 실험을 통하여 살펴보았다. 역전사효소 억제활성을 ELOSA 방법으로 실험한 결과 능소화 줄기의 물 추출물이 100 ㎍/㎖농도에서 각각 37.9%의 활성을 나타내었고, 능소화 잎과줄기의 메탄올 추출물에서 33.6% 및 31.5%의 HIV-1 protease 억제활성 나타 내었다. 그리고 HIV-1 복제 억제활성은 MT-4 세포에 대한 HIV-1 유도 세포변성억제를 광학현미경으로 관찰하여 살펴본 결과 줄기의 물 추출물이100 ㎍/㎖ 농도에서 HIV-1 바이러스 증식을 완전히 억제하였다.
Abstract
For the elucidation of action mechanism on anti-HIV of natural resources, the extracts of Campsis grandiflora were tested for their inhibitory effects on HIV-1 replication and its essential enzymes as the reverse transcriptase (RT),protease and α- glucosidase. In the assay of HIV-1-infected human T-cell line, water extracts of stem inhibited the HIV-1-induced cytopathic effects with IC (inhibitory concentration) of 100 ㎍/ml. Moreover water extracts (100 ㎍/ml) of stem showed strong activity of 37.9% on anti-HIV-1 RT using Enzyme Linked Oligonucleotide Sorbent Assay (ELOSA)method. In the HIV-1 protease inhibition assay, methanol extracts of stem and leaf extract showed 33.6% and 31.5%inhibition of the enzyme activity to cleave an oligopeptide resembling one of the cleavage sites in the viral polyprotein which can only be processed by HIV-1 protease, but did not exhibited glucosidase inhibitory activities. From these results,it is suggested that the inhibition of the viral replication in vitro is due to the inhibition of reverse transcriptase by water extracts of stem of Campsis grandiflora.
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