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Antioxidant and Antibacterial Effects of Mixed Extracts of Phyllanthus emblica, Geranium (Pelargonium graveolens) and Commiphora myrrha: Possibility of Natural Materials for Acne Treatment

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초록

Various skin diseases are occurring due to external factors such as urbanization and increase in environmental pollution and wearing masks due to COVID-19. Accordingly, various functional cosmetics are being released, but as some side effects are reported, research on functional cosmetics materials using natural plants is necessary. Therefore, in this study, the antioxidant, antibacterial and anti-inflammatory effects of Phyllanthus emblica, Geranium (Pelargonium graveolens), and Commiphora myrrha mixed extracts (PGC) that pharmacological efficacy has been verified were analyzed and their potential as functional cosmetics raw materials was examined. Four extracts (PGC-1~4) were prepared according to the extraction method. ABTS and DPPH radical scavenging activity experiments were conducted for the antioxidant efficacy of the extracts. In addition, paper disc experiments and LPS inflammation-inducing cytokine experiments were conducted to examine the antibacterial and anti-inflammatory effects. In addition, a cell viability test was performed to confirm cytotoxicity. As results of the study, all extracts showed antioxidant, antibacterial, and anti-inflammatory effects without cytotoxicity, and in particular, PGC-4, a fermentation and ultrasonic extract, showed the best efficacy. This means that the extraction yield of useful components varies depending on the extraction method.

Abstract

Various skin diseases are occurring due to external factors such as urbanization and increase in environmental pollution and wearing masks due to COVID-19. Accordingly, various functional cosmetics are being released, but as some side effects are reported, research on functional cosmetics materials using natural plants is necessary. Therefore, in this study, the antioxidant, antibacterial and anti-inflammatory effects of Phyllanthus emblica, Geranium (Pelargonium graveolens), and Commiphora myrrha mixed extracts (PGC) that pharmacological efficacy has been verified were analyzed and their potential as functional cosmetics raw materials was examined. Four extracts (PGC-1~4) were prepared according to the extraction method. ABTS and DPPH radical scavenging activity experiments were conducted for the antioxidant efficacy of the extracts. In addition, paper disc experiments and LPS inflammation-inducing cytokine experiments were conducted to examine the antibacterial and anti-inflammatory effects. In addition, a cell viability test was performed to confirm cytotoxicity. As results of the study, all extracts showed antioxidant, antibacterial, and anti-inflammatory effects without cytotoxicity, and in particular, PGC-4, a fermentation and ultrasonic extract, showed the best efficacy. This means that the extraction yield of useful components varies depending on the extraction method.

참고문헌 (46)

  1. 배창희, "미용관련 피부질환 인식을 위한 CNN기반 딥러닝 알고리즘의 실험적 비교", 한국컴퓨터정보학회논문지, 25(12), pp.25~34 (2020), 한국컴퓨터정보학회[DOI]
  2. Pande G, "Organic acids, antioxidant capacity, phenolic content and lipid characterisation of Georgia-grown underutilized fruit crops", Food Chem, 120, pp.1067~1075 (2010)
  3. Dasaroju S, "Current trends in the research of Emblica officinalis (Amla): A pharmacological perspective", Int. J. Pharm. Sci. Rev. Res, 24, pp.150~159 (2014)
  4. 김수찬, "피부외용제에 의한 부작용 사례 연구", 대한피부과학회지, 41(9), pp.1129~1135 (2003), 대한피부과학회
  5. Zaenglein AL, "Acne vulgaris", New Eng. J. Med, 379, pp.1343~1352 (2018)
  6. Webster GF, "Acne vulgaris: state of the science", Arch. Dermatol, 135, pp.1101~1102 (1995)
  7. Brown SK, "Acne vulgaris", Lancet, 351, pp.1871~1876 (1998)
  8. 마충제, "여드름 원인균 Propionibacterium acnes에 대한 생약 추출물의 항균활성 측정", 생약학회지, 42(1), pp.98~101 (2011), 한국생약학회
  9. Lee NH, "A clinical study on the effect of a facial cleanser consisting of 1% triclosan and 0.5% Ku Shen on acne vulgaris", Korean J. Dermatol, 36, pp.871~876 (1998)
  10. 임미혜, "Benzoyl peroxide lotion 의 여드름 피부 적용 후 pH, 유분, 수분의 변화", 아시안뷰티화장품학술지, 3(1), pp.37~48 (2005), 한국피부과학연구원
  11. Bojar RA, "The in-vitro anti-microbial effects of azelaic acid upon Propionibacterium acnes strain P37", J. Antimicrob. Chemother, 28, pp.843~853 (1991)
  12. Aires JR, "Amino acid residues essential for function of the MexF efflux pump protein of Pseudomonas aeruginosa", Antimicrob. Agents Chemother, 46, pp.2169~2173 (2002)
  13. Leyden JJ, "The antimicrobial effects in vivo of minocycline, doxycycline and tetracycline in humans", J. Dermatol. Treatment, 7, pp.223~225 (1996)
  14. 임영희, "In vitro Activity of Kaempferol Isolated from the Impatiens balsamina alone and in Combination with Erythromycin or Clindamycin against Propionibacterium acnes", The Journal of Microbiology, 45(5), pp.473~477 (2007), 한국미생물학회
  15. Kobayashi M, "Effects of oral antibiotic roxithromycin on quality of life in acne patients", J. Dermatol, 36, pp.383~391 (2009)
  16. Dasaroju S, "Current trends in the research of Emblica officinalis (CP): A pharmacological perspective", Int. J. Pharm. Sci. Rev. Res, 24, pp.150~159 (2014)
  17. Tsuji N, "The role of elastases secreted by fibroblasts in wrinkle formation:implication through selective inhibition of elastase activity", Photochem. Photobiol, 74, pp.283~290 (2001)
  18. Gates MA, "A prospective study of dietary flavonoid intake and incidence of epithelial ovarian cancer", Int. J. Cancer, 121, pp.2225~2232 (2007)
  19. Akdemir ZS, "Polyphenolic compound from Geranium pratense and their free radical scavenging activities", Phytochemistry, 56, pp.189~193 (2001)
  20. Prabuseenivasan S, "In vitro antibacterial activity of some plant essential oils", BMC Complement. Altern. Med, 6, pp.39~ (2006)
  21. Su S, "Antiinflammatory and analgesic activity of different extracts of Commiphora myrrha", J. Ethnopharmacol, 134, pp.251~258 (2011)
  22. Sotoudeh R, "The anti-diabetic and antioxidant effects of a combination of Commiphora mukul, Commiphora myrrha and Terminalia chebula in diabetic rats", Avicenna J. Phytomed, 9, pp.454~464 (2019)
  23. Madia VN, "Investigation of Commiphora myrrha (Nees) Engl. oil and its main components for antiviral activity", Pharmaceuticals, 14, pp.243~254 (2021)
  24. Que F, "Antioxidant activities of five Chinese rice wines and the involvement of phenolic compounds", Food Res. Int, 39, pp.581~587 (2006)
  25. Arnao MB, "Some methodological problems in the determination of antioxidant activity using chromogen radicals: a practical case", Trends Food Sci. Technol, 11, pp.419~421 (2000)
  26. Jeong JA, "Screening for anti-oxidative activities of extracts from aerial and underground parts of some edible and medicinal ferns", Korean J. Plant Resour, 20, pp.185~192 (2007)
  27. Aoshima H, "Aging of whiskey increases 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity", J. Agric. Food Chem, 52, pp.5240~5244 (2004)
  28. 김미경, "운향과 과실 추출물의 항균활성 조사", 한국컴퓨터정보학회논문지, 26(12), pp.237~245 (2021), 한국컴퓨터정보학회[DOI]
  29. 왕승진, "막걸리로부터 항산화 화합물의 단리·정제 및 동정", 한국식품과학회지, 44(1), pp.14~20 (2012), 한국식품과학회
  30. Guzik TJ, "Nitric oxide and superoxide in inflammation and immune regulation", J. Physiol. Pharmacol, 43, pp.469~487 (2003)
  31. Graca VC, "Phytochemical composition and biological activities of Geranium robertianum L.:A review", Ind. Crops Prod, 87, pp.363~378 (2016)
  32. Hamed AM, "Buffalo yogurt fortified with Eucalyptus (Eucalyptus camaldulensis) and Myrrh (Commiphora Myrrha)essential oils: New insights into the functional properties and extended shelf life", Molecules, 26, pp.6853~ (2021)
  33. Fu LW, "Newer approaches in topical combination therapy for acne", Skin Ther. Lett, 16, pp.3~6 (2011)
  34. Koreck A, "The role of innate immunity in the pathogenesis of acne", Dermatology, 206, pp.96~105 (2003)
  35. Witold M, "Preliminary assessment of alginic acid as a factor buffering triethanol amine interacting with artificial skin sebum", Eur. J. Pharm. Biopharm, 55, pp.237~240 (2003)
  36. 김나래, "천연물유래 여드름 치료제제의 항균활성 측정", 한국미생물·생명공학회지, 37(1), pp.80~84 (2009), 한국미생물·생명공학회
  37. 이주연, "여드름 피부에 효능 및 안전성을 가진 성분의 연구동향", 아시안뷰티화장품학술지, 16(3), pp.449~463 (2018), 한국피부과학연구원[DOI]
  38. Chan GY, "Antimicrobial effects of extracts of Taraxacum officinale H. on acnes strains", Int. J. Integrated CAM, 7, pp.3~16 (2011)
  39. Laksmitawati DR, "Anti-inflammatory potential of gandarusa (Gendarussa vulgaris Nees) and soursoup (Annona muricata L) extracts in LPS stimulated-macrophage cell (RAW264. 7)", J. Nat. Remedies, 16, pp.73~81 (2016)
  40. 김승희, "LPS로 유도된 RAW264.7 세포주 염증모델에서 소리쟁이의 법제처리에 따른 항염증 효과", 한국식품영양학회지, 32(5), pp.408~416 (2019), 한국식품영양학회[DOI]
  41. 장귀영, "열처리와 추출방법에 따른 몇 가지 약초의 항산화 활성", 한국식품영양과학회지, 41(7), pp.914~920 (2012), 한국식품영양과학회[DOI]
  42. Chemat F, "Applications of ultrasound in food technology: processing, preservation and extraction", Ultrason. Sonochem, 18, pp.813~835 (2011)
  43. Kim HY, "Effects of Oenothera odorata on vasorelaxation in thoracic and carotid artery", FASEB Exp. Biol. J, 23, pp.939~918 (2009)
  44. 김진학, "추출방법을 달리한 달맞이꽃 추출물의 항산화 및 항균 활성", Food Science and Preservation, 23(2), pp.233~238 (2016), 한국식품저장유통학회
  45. Klein A, "Prolonged stabilization of platinum-resistant ovarian cancer in a single patient consuming a fermented soy therapy", Gynecol. Oncol, 100, pp.205~209 (2006)
  46. 박미란, "발효천마의 총 폴리페놀 함량 변화 및 라디칼 소거능", 한국자원식물학회지, 25(4), pp.379~386 (2012), 한국자원식물학회[DOI]

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