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Production of nitric oxide by murine macrophages induced by lipophosphoglycan of Leishmania major

Sussan K. Ardestani|Amina Kariminia|Gholamreza Kavoosi|Zahra Tavakoli
대한기생충학ㆍ열대의학회 (The Korean Society For Parasitology and Tropical Medicine)|2006.03|Parasites, Hosts and Diseases|제44권 제1호|pp. 35-41

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참고문헌

주제분류예방의학/직업환경의학

초록

Abstract

Protozoan parasites of the genus Leishmania cause a number of important human diseases. One of thekey determinants of parasite infectivity and survival is the surface glycoconjugate lipophosphoglycan (LPG). In addi-tion, LPG is shown to be useful as a transmission blocking vaccine. Since culture supernatant of parasite promastig-otes is a good source of LPG, we made attempts to characterize functions of the culture supernatant, and membraneLPG isolated from metacyclic promastigotes of Leishmania major. The purification scheme included anion-exchangechromatography, hydrophobic interaction chromatography and cold methanol precipitation. The purity of supernatantLPG (sLPG) and membrane LPG (mLPG) was determined by SDS-PAGE and thin layer chromatography. The effectof mLPG and sLPG on nitric oxide (NO) production by murine macrophages cell line (J774.1A) was studied. BothsLPG and mLPG induced NO production in a dose dependent manner but sLPG induced significantly higher amountof NO than mLPG. Our results show that sLPG is able to promote NO production by murine macrophages.

참고문헌 (21)

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