감마선처리 냉동 우육, 분쇄우육 및 떡갈비의 판별을 위한 Comet assay의 적용성 평가
Evaluation on the Applicability of Comet Assay for the Identification of Gamma-irradiated Frozen Beef Cut, Ground Beef and Tteokgalbi
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초록
This study was conducted to evaluate the applicability of the Comet assay, which is widelyused for the identification of irradiated meats, to detect irradiated beef cut, ground beef, and Tteokgalbiduring freezing storage. Gamma-irradiation significantly increased the DNA damage in frozen beef cutand ground beef samples. Among those, DNA nuclei of samples irradiated with absorbed doses of 1 kGyor more showed typical comet-shaped damage, convincing that the samples were irradiated. Meanwhile,DNA nuclei in non-irradiated beef cut and ground beef samples were also damaged according to storagetime. In particular, since the damage of DNA nuclei in the non-irradiated samples frozen for threemonths was similar to that of samples irradiated with a dose of 0.5 kGy, it was considered difficult todetect whether these samples were irradiated by Comet assay analysis. Likewise, gamma-irradiationof Tteokgalbi increased DNA damage. However, significant damage to DNA nuclei was observed even inthe non-irradiated samples. Therefore, the application of the analysis method to determine whether theTteokgalbi sample was irradiated was not appropriate. In conclusion, these results suggest that Cometassay could be limitedly applied only to fresh meat with a short storage period and minimal processing.
Abstract
This study was conducted to evaluate the applicability of the Comet assay, which is widelyused for the identification of irradiated meats, to detect irradiated beef cut, ground beef, and Tteokgalbiduring freezing storage. Gamma-irradiation significantly increased the DNA damage in frozen beef cutand ground beef samples. Among those, DNA nuclei of samples irradiated with absorbed doses of 1 kGyor more showed typical comet-shaped damage, convincing that the samples were irradiated. Meanwhile,DNA nuclei in non-irradiated beef cut and ground beef samples were also damaged according to storagetime. In particular, since the damage of DNA nuclei in the non-irradiated samples frozen for threemonths was similar to that of samples irradiated with a dose of 0.5 kGy, it was considered difficult todetect whether these samples were irradiated by Comet assay analysis. Likewise, gamma-irradiationof Tteokgalbi increased DNA damage. However, significant damage to DNA nuclei was observed even inthe non-irradiated samples. Therefore, the application of the analysis method to determine whether theTteokgalbi sample was irradiated was not appropriate. In conclusion, these results suggest that Cometassay could be limitedly applied only to fresh meat with a short storage period and minimal processing.
참고문헌 (25)
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