본문 바로가기
KERD
논문KCI 등재

Protective effect of Oxya chinensis sinuosa methanol extract on UVB-induced damage in human retinal pigment epithelial cells

2

조회

0

다운로드

1

인용

0

참고문헌

키워드

초록

The human eye, constantly exposed to solar radiation, can be damaged by UV radiation. In particular, ultraviolet B (UVB)-induced damage plays an important role in retinal degeneration and cell aging. In this study, we investigated the protective effects of the methanol extract of Oxya chinensis sinuosa (OCM), an edible insect known for its high protein content (64.2%), and various pharmacological effects, on human retinal pigment epithelial cells. ARPE-19 cells were treated with OCM and subsequently UVB irradiated. Our results showed that OCM effectively attenuates UVB-induced cell damage by reducing MAPK phosphorylation (JNK and p38 MAPK). Additionally, OCM increased the phosphorylation of Akt, and cell cycle regulators, including p21 and p27, in a dose-dependent manner. Moreover, OCM treatment increased ARPE-19 cell proliferation by activating the S6K1/S6 pathway. This study suggests that OCM prevents UVB-induced retinal cell damage by increasing cell proliferation via ROS reduction, suggesting its potential as a functional therapeutic superfood against retinal cell damage.

Abstract

The human eye, constantly exposed to solar radiation, can be damaged by UV radiation. In particular, ultraviolet B (UVB)-induced damage plays an important role in retinal degeneration and cell aging. In this study, we investigated the protective effects of the methanol extract of Oxya chinensis sinuosa (OCM), an edible insect known for its high protein content (64.2%), and various pharmacological effects, on human retinal pigment epithelial cells. ARPE-19 cells were treated with OCM and subsequently UVB irradiated. Our results showed that OCM effectively attenuates UVB-induced cell damage by reducing MAPK phosphorylation (JNK and p38 MAPK). Additionally, OCM increased the phosphorylation of Akt, and cell cycle regulators, including p21 and p27, in a dose-dependent manner. Moreover, OCM treatment increased ARPE-19 cell proliferation by activating the S6K1/S6 pathway. This study suggests that OCM prevents UVB-induced retinal cell damage by increasing cell proliferation via ROS reduction, suggesting its potential as a functional therapeutic superfood against retinal cell damage.

참고문헌 (56)

  1. Abukhdeir AM, "P21 and p27 : roles in carcinogenesis and drug resistance", Expert Rev Mol Med, 10, pp.e19~ (2008)[DOI]
  2. Biesemeier A, "UV-A induced oxidative stress is more prominent in naturally pigmented aged human RPE cells compared to non-pigmented human RPE cells independent of zinc treatment", J Photochem Photobiol B : Biol, 90, pp.113~120 (2008)[DOI]
  3. Birben E, "Oxidative stress and antioxidant defense", World Allergy Organ J, 5, pp.9~19 (2012)[DOI]
  4. Bode AM, "Mitogen-activated protein kinase activation in UV-induced signal transduction", Sci STKE, 28, pp.RE2~ (2003)[DOI]
  5. Brown EJ, "Control of p70 s6 kinase by kinase activity of FRAP in vivo", Nature, 377, pp.441~446 (1995)[DOI]
  6. Burnett PE, "RAFT1 phosphorylation of the translational regulators p70 S6 kinase and 4E-BP1", Proc Natl Acad Sci U S A, 95, pp.1432~1437 (1998)[DOI]
  7. Cao G, "EGCG protects against UVB-induced apoptosis via oxidative stress and the JNK1", c-Jun pathway in ARPE19 cells, 2012, pp.5, 54~59
  8. Carnero A, "The PKB", AKT pathway in cancer, 2010, pp.16, 34~44
  9. Carr TD, "Inhibition of mammalian target of rapamycin(mTOR)suppresses UVB-induced keratinocyte proliferation and survival", Cancer Prev Res (Phila), 5, pp.1394~1404 (2012)[DOI]
  10. 정경수, "벼메뚜기(Oxya japonica japonica Thunberg) 물 추출물 LCT-CT의 항암면역 활성에 관한 유세포 분석학적 연구", 약 학 회 지, 58(3), pp.151~157 (2014), 대한약학회
  11. Costantini L, "Impact of omega-3 fatty acids on the gut microbiota", Int J Mol Sci, 18, pp.2645~ (2017)[DOI]
  12. DeBerardinis RJ, "The biology of cancer : metabolic reprogramming fuels cell growth and proliferation", Cell Metab, 7, pp.11~20 (2008)[DOI]
  13. Fernandes SA, "The multifaceted role of nutrient sensing and MTORC1 signaling in physiology and aging", Front Aging, 2, pp.38~ (2021)[DOI]
  14. Fitsiou E, "Cellular senescence and the senescence-associated secretory phenotype as drivers of skin photoaging", J Investig Dermatol, 141, pp.1119~1126 (2021)[DOI]
  15. Fruman DA, "PI3K and cancer : lessons, challenges and opportunities", Nat Rev Drug Discov, 13, pp.140~156 (2014)[DOI]
  16. Godar DE, "Long-wavelength UVA radiation induces oxidative stress, cytoskeletal damage and hemolysis", Photochem Photobiol, 57, pp.1018~1026 (1993)[DOI]
  17. Hao XN, "Effects of resveratrol on ARPE-19 cell proliferation and migration via regulating the expression of proliferating cell nuclear antigen, P21, P27 and p38MAPK", MMP-9, 2016, pp.9, 1725~1731
  18. Hseu YC, "Antrodia camphorate inhibits proliferation of human breast cancer cells in vitro and in vivo", Food Chem Toxicol, 46, pp.2680~2688 (2008)[DOI]
  19. Hsieh FC, "Protective effects of Lycium barbarum extracts on UVB-induced damage in human retinal pigment epithelial cells accompanied by attenuating ROS and DNA damage", Oxid Med Cell Longev, 2018, pp.4814928~ (2018)[DOI]
  20. Im AR, "Protective effects of Oxya chinensis sinuosa Mishchenko against untraviolet B-induced photodamage in hairless mice", BMC Complement Altern Med, 19, pp.286~ (2019)[DOI]
  21. Kadenbach B, "Degenerative diseases, oxidative stress and cytochrome c oxidase function", Trends Mol Med, 15, pp.139~147 (2009)[DOI]
  22. Kaunppinen A, "Oxidative stress activates NLRP3 inflammasomes in ARPE-19 cells-implications for age-related macular degeneration(AMD)", Immunol Lett, 147, pp.29~33 (2012)[DOI]
  23. 김아영, "인류의 생존 위기 대응을 위한 양잠과 곤충 산업의 현황", 한국응용곤충학회지, 61(4), pp.605~614 (2022), 한국응용곤충학회[DOI]
  24. 김봉선, "ARPE-19 세포에서 산화적 스트레스에 대한 갈색거저리 추출물의 항산화 효과", 생명과학회지, 32(11), pp.865~871 (2022), 한국생명과학회[DOI]
  25. Kim HJ, "Antioxidant activity and antimicrobial activity of the grasshopper, Oxya chinensis sinuosa", J Seric Entomol Sci, 53, pp.130~134 (2015)[DOI]
  26. 김하림, "약콩, 비트 추출물의 자외선에 의한 망막 상피세포와 마우스의 눈 손상 조절 효능", 생약학회지, 51(2), pp.131~138 (2020), 한국생약학회[DOI]
  27. 김인우, "Transcriptome Profiling and In Silico Analysis of the Antimicrobial Peptides of the Grasshopper Oxya chinensis sinuosa", Journal of Microbiology and Biotechnology, 26(11), pp.1863~1870 (2016), 한국미생물·생명공학회
  28. Kiyokawa H, "Enhanced growth of mice lacking the cyclin-dependent kinase inhibitor function of p27(Kip1)", Cell, 85, pp.721~732 (1996)[DOI]
  29. Klopfleisch R, "Differential expression of cell cycle regulators p21, p27 and p53 in metastasizing canine mammary adenocarcinomas versus normal mammary glands", Res Vet Sci, 87, pp.91~96 (2009)[DOI]
  30. Koskela A, "Nutraceutical with resveratrol and omega-3 fatty acids induces autophagy in ARPE-19 cells", Nutrients, 8, pp.284~ (2016)[DOI]
  31. Laplante M, "mTOR signaling at a glance", J Cell Sci, 122, pp.3589~3594 (2009)[DOI]
  32. Lee KS, "The preventive effect of deep sea water on the development of cancerous skin cells through the induction of autophagic cell death in UVB-damaged HaCaT keratinocyte", Biomed Pharmacother, 111, pp.282~291 (2019)[DOI]
  33. 이지혜, "Analysis of nutrient composition of silkworm pupae in Baegokjam, Goldensilk, Juhwangjam, and YeonNokjam varieties", International Journal of Industrial Entomology and Biomaterials, 43(2), pp.85~93 (2021), 한국잠사학회[DOI]
  34. Lei X, "UVB-induced p21degradation promotes apoptosis of human keratinocytes", Photochem Photobiol Sci, 9, pp.1640~1648 (2010)[DOI]
  35. Li T, "Ultraviolet-induced junD activation and apoptosis in myeloblastic leukemia ML-1 cells", J Biol Chem, 277, pp.32668~32676 (2002)[DOI]
  36. Liu H, "Protective effect of lutein on ARPE-19 cells upon H2O2-induced G2", M arrest, 2017, pp.16, 2069~2074
  37. Marie M, "Light action spectrum on oxidative stress and mitochondrial damage in A2E-loaded retinal pigment epithelium cells", Cell Death Dis, 9, pp.287~299 (2018)[DOI]
  38. Nilsson SEG, "Aging of cultured retinal pigment epithelial cells : oxidative reactions, lipofuscin formation and blue light damage", Doc Ophthalmol, 106, pp.13~16 (2003)[DOI]
  39. Nowakowsk AC, "Potential health benefits of edible insects", Crit Rev Food Sci Nutr, 62, pp.3499~3508 (2022)[DOI]
  40. Osaki M, "PI3K-Akt pathway : its functions and alterations in human cancer", Apoptosis, 9, pp.667~676 (2004)[DOI]
  41. Oshiro N, "The proline-rich Akt substrate of 40 kDa(PRAS40)is a physiological substrate of mammalian target of rapamycin complex1", J Biol Chem, 282, pp.20329~20339 (2007)[DOI]
  42. 박자영, "메뚜기류 추출물의 염증 조절작용 및 세포사멸보호 효과", Food Science and Preservation, 13(6), pp.796~802 (2006), 한국식품저장유통학회
  43. Roduit R, "MAP kinase pathways in UV-induced apoptosis of retinal pigment epithelium ARPE19 cells", Apoptosis, 13, pp.343~353 (2008)[DOI]
  44. Sherr CJ, "The RB and p53 pathways in cancer", Cancer cell, 2, pp.103~112 (2002)[DOI]
  45. Silván JM, "A protective effect of anthocyanins and xanthophylls on UVB-induced damage in retinal pigment epithelial cells", Food & Feunct, 7, pp.1067~1076 (2016)[DOI]
  46. Sparrow JR, "The lipofuscin fluorophore A2E mediates blue light-induced damage to retinal pigmented epithelial cells", IOVS, 41, pp.1981~1989 (2000)
  47. Strauss O, "The retinal pigment epithelium in visual function", Physiol Rev, 85, pp.845~881 (2005)[DOI]
  48. Strimpakos AS, "The role of mTOR in the management of solid tumors : an overview", Cancer Treat Rev, 35, pp.148~159 (2009)[DOI]
  49. Stull VJ, "Impact of edible cricket consumption on gut microbiota in healthy adults, a double-blind, randomized crossover trial", Sci Rep, 8, pp.10762~ (2018)[DOI]
  50. Stull VJ, "Chitin and omega-3 fatty acids in edible insects have underexplored benefits for the gut microbiome and human health", Nat Food, 4, pp.283~287 (2023)[DOI]
  51. Viglietto G, "Understanding p27(kip1)deregulation in cancer : down-regulation or mislocalization", Cell Cycle, 1, pp.394~400 (2002)[DOI]
  52. Wang Y, "mTOR signaling pathway : a potential therapeutic target for ocular neurodegenerative diseases", Antioxidant(Basel), 11, pp.1304~ (2022)
  53. Whang HJ, "Quantitative analysis of total phenolic content in apple", Anlytical Sci Technol, 14, pp.377~383 (2001)
  54. Xu JY, "Green tea polyphenols attenuating ultraviolet B-induced damage to human retinal pigment epithelial cells in vitro", Invest Opthamol Vis Sci, 51, pp.6665~6670 (2010)[DOI]
  55. 윤영일, "LPS로 유도된 RAW 264.7세포에 대한 벼메뚜기(Oxya chinensis sinuosa) 에탄올 추출물의 항염증 효과", 생명과학회지, 24(4), pp.370~376 (2014), 한국생명과학회
  56. Zwang Y, "p38 MAP kinase mediates stress-induced internalization of EGFR : implications for cancer chemotherapy", EMBO J, 25, pp.4195~4206 (2006)[DOI]

관련 논문AI추천

Anti-inflammatory and antioxidant effects of Oxya chinensis sinuosa methanol extract on LPS-induced HT-29 human colon adenocarcinoma cells권해용, 김용순, 김인우, 서민철, 이준하, 임현정한국잠사학회 · International Journal of Industrial Entomology and Biomaterials2024 · 제48권 제4호Protective Effects of Epigallocatechin Gallate after UV Irradiation in Cultured Human Retinal Pigment Epithelial CellsByung Rae Lee, Jae-Woong Koh, Seong-Won Yang대한안과학회 · Korean Journal of Ophthalmology2007 · 제21권 제4호Kjellmaniella crassifolia for the Prevention of Ultraviolet B-Induced Oxidative Stress in HaCaT KeratinocytesAhn Ginnae, Hyun Jimin, Jang Mi-Soon, Jayasinghe Arachchige Maheshika Kumari, Kirindage Kirinde Gedara Isuru Sandanuwan, Oh Jae-Young, Park Kunbawui, Ryu BoMi, Yun Hyun-Jung한국미생물·생명공학회 · Journal of Microbiology and Biotechnology2025 · 제35권 Astaxanthin Protects Ultraviolet B-Induced Oxidative Stress and Apoptosis in Human Keratinocytes via Intrinsic Apoptotic Pathway유동수, 윤소연, 이영복, Chung Bom Yee, Park Sang Ho대한피부과학회 · Annals of Dermatology2022 · 제34권 제2호Anthocyanins Inhibits Oxidative Injury in Human Retinal Pigment Epithelial ARPE-19 Cells via Activating Heme Oxygenase-1Choi Yung Hyun, Hong Sang Hoon, Hong Su Hyun, Hwangbo Hyun, Kim Gi-Young, Kim Sung Ok, Noh Jeong Sook, Park Cheol, Park Shin-Hyung한국미생물·생명공학회 · Journal of Microbiology and Biotechnology2024 · 제34권 제3호Chlorella vulgaris 미세조류 추출물의 자외선B 의존적 인간 진피섬유아 세포 손상에 대한 보호 효능 연구김광년, 김기쁨, 김수영, 김희식, 배승희, 안규중, 안성관, 안인숙, 이정주, 조대현, 허진아한국피부과학연구원 · 아시안뷰티화장품학술지2014 · 제12권 제4호

이 간행물의 다른 논문 (8건)