Enhanced tumor formation of YD-15 mucoepidermoid carcinoma cells via intradermal injection in nude mice: a preliminary study
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The YD-15 cell line is derived from a mucoepidermoid carcinoma of the tongue. It has historically shown extremely low tumor formation in vivo (0% take rate in nude mice). This feature poses a challenge for developing xenograft models to test new treatments for this cancer. Thus, this study aimed to establish a reliable tumor xenograft model for YD-15 cells by exploring an alternative implantation technique. Specifically, this study aimed to evaluate whether an intradermal injection method could improve tumorigenicity when standard subcutaneous injection fails. This preliminary study reveals that intradermal injections can substantially improve the in vivo tumorigenicity of YD-15 mucoepidermoid carcinoma cells. The intradermal technique presents as a simple and effective alternative to achieve tumor formation if conventional subcutaneous xenografting for low-tumorigenic cell lines is not successful. However, the approach should be validated in independent cohorts and compared with other methods in future research.
Abstract
The YD-15 cell line is derived from a mucoepidermoid carcinoma of the tongue. It has historically shown extremely low tumor formation in vivo (0% take rate in nude mice). This feature poses a challenge for developing xenograft models to test new treatments for this cancer. Thus, this study aimed to establish a reliable tumor xenograft model for YD-15 cells by exploring an alternative implantation technique. Specifically, this study aimed to evaluate whether an intradermal injection method could improve tumorigenicity when standard subcutaneous injection fails. This preliminary study reveals that intradermal injections can substantially improve the in vivo tumorigenicity of YD-15 mucoepidermoid carcinoma cells. The intradermal technique presents as a simple and effective alternative to achieve tumor formation if conventional subcutaneous xenografting for low-tumorigenic cell lines is not successful. However, the approach should be validated in independent cohorts and compared with other methods in future research.