구강암 세포주의 이소위 누드마우스 종양 모델
AN ATOPIC NUDE MOUSE MODEL OF ORAL CANCER CELL LINE
2
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
In order to make successful oral cancer treatment, we need to understand about tumor biology and effective chemotherapeutic agents. To achieve these studies, it is necessary to develope a proper in-vivo model. Therefore the author will make try to develop more improved animal model of more applicable in various method of cancer study. In this study, the author induced in-vivo tumorigenesis in nude mice by YD-10Bmod cell line used by YD-10B cell line originated from oral tongue squamous cell carcinoma and observed tumor formations and invasiveness of surrounding tissue, and found some results as follows : 1. The experimental group (YD-10Bmod, subcutaneous injection) produced tumors 13 out of 15 mice, while the control group produced none of 5 mice. 2. The inoculation of 1×106cells/mouse produced tumors 3 out of 5 mice and inoculation of 1×107cells/mouse, 2×107cells/mouse produced tumors in every 5 mice. 3. In the histopathologic studies, the inoculation of 1×106cells/mouse group showed the characteristic features of well-differentiated squamous cell carcinoma and demarcated expansile growth, while the inoculation of 1×107cells/mouse, 2×107cells/mouse group showed the expansile growth with partial central necrosis and invasive growth to surrounding fat & connective tissue. These findings suggest that atopic xenograft of YD-10Bmod cell line in nude mice has a improved productivity of tumors, produced tumors showed the characteristics feature of human tumor and invasive growth to surrounding tissue in histopathologic appearance. These atopic nude mouse model of tongue carcinoma might assist in studying oral cancer biology and effective choice of chemotherapeutic agents.
Abstract
In order to make successful oral cancer treatment, we need to understand about tumor biology and effective chemotherapeutic agents. To achieve these studies, it is necessary to develope a proper in-vivo model. Therefore the author will make try to develop more improved animal model of more applicable in various method of cancer study. In this study, the author induced in-vivo tumorigenesis in nude mice by YD-10Bmod cell line used by YD-10B cell line originated from oral tongue squamous cell carcinoma and observed tumor formations and invasiveness of surrounding tissue, and found some results as follows : 1. The experimental group (YD-10Bmod, subcutaneous injection) produced tumors 13 out of 15 mice, while the control group produced none of 5 mice. 2. The inoculation of 1×106cells/mouse produced tumors 3 out of 5 mice and inoculation of 1×107cells/mouse, 2×107cells/mouse produced tumors in every 5 mice. 3. In the histopathologic studies, the inoculation of 1×106cells/mouse group showed the characteristic features of well-differentiated squamous cell carcinoma and demarcated expansile growth, while the inoculation of 1×107cells/mouse, 2×107cells/mouse group showed the expansile growth with partial central necrosis and invasive growth to surrounding fat & connective tissue. These findings suggest that atopic xenograft of YD-10Bmod cell line in nude mice has a improved productivity of tumors, produced tumors showed the characteristics feature of human tumor and invasive growth to surrounding tissue in histopathologic appearance. These atopic nude mouse model of tongue carcinoma might assist in studying oral cancer biology and effective choice of chemotherapeutic agents.
참고문헌 (38)
- Johnson NW, "Global epidemiology in oral cancer", pp.10~20 (2003), Martin Dunitz
- Carew JF, "Factors affecting choice of treatment. In Oral Cancer", pp.209~213 (2003), Martin Dunitz
- Hawkins BL, "4NQO carcinogenesis : A mouse model of oral cavity squamous cell carcinoma", Head Neck, 16, pp.424~432 (1994)
- Giovanella BC, "Heterotransplantation of human malignant tumors in nude thymusless mice 1. Breathing and maintenance of nude mice", J Natl Cancer Inst, 51, pp.615~619 (1973)
- Giovanella BC, "Heterotransplantation of human malignant tumors in nude thymusless mice. II. Malignant tumors induced by injection of cell cultures derived from human solid tumors", J Natl Cancer Inst, 52, pp.921~930 (1974)
- Santis H, "Histochemistry of experimentary induced leukoplakia and carcinoma of the hamster buccal pouch", Oral Surg, 17, pp.207~218 (1964)
- Odukoya O, "Two-phase carcinogenesis in hamster buccal pouch", Oral Surg, 54, pp.547~552 (1982)
- Fidler IJ, "Rationale and methods for the use of nude mice to study the biology and therapy of human cancer metastasis", Cancer Metastasis Rev, 5, pp.29~49 (1986)
- Rygaard J, "Heterotransplantation of a human malignant tumour to “Nude”mice", Acta Pathol Microbiol Scand, 77(4), pp.758~760 (1969)
- McDermott-Lancaster RD, "The nude mouse characteristics, breeding and husbandary", Int J Leprosy, 55, pp.885~888 (1987)
- Hendrickson EA, "The SCID mouse : relevance as an animal model system for studying human disease", Am J Pathol, 143, pp.1151~ (1993)
- Kubota T, "Metastastic models of human cancer xenografted in mice: the importance of orthotopic transplantation", J Cell Biochem, 56, pp.4~ (1994)
- Fu XY, "Extensive multi organ metastasis following orthotopic onplantation of histologically intact human bladder carcinoma tissue in nude mice", Int J Cancer, 49, pp.938~ (1991)
- Umeda M, "Establishment and characterization of a human adenoid cystic carcinoma line of the salivary gland which is serially transplantable and spontaneously metastasis to the lung in nude mice", Oral Oncol, 38, pp.30~40 (2002)
- Kim S, "An Orthotopic model of anaplastic thyroid carcinoma", Clin Cancer Res, 11, pp.1713~1721 (2005)
- Khanna C, "Modeling metastasis in vivo", Carcinogenesis, 26(3), pp.513~523 (2005)
- Killion JJ, "Orthotopic models are necessary to predict therapy of transplantable tumors in mice", Cancer metastasis Rev, 17, pp.279~284 (1998)
- Scheper MA, "Sulindac induces apoptosis and inhibits tumor growth in vivo in head and neck Squamous cell carcinoma", Neoplasia, 9(3), pp.192~199 (2007)
- Huang SM, "Modulation of radiation response after epidermal growth factor recept or blockade in squamous cell carcinomas : inhibition of damage repair, cell cycle kinetics, and tumor angiogenesis", Clin Cancer Res, 6(6), pp.2166~2174 (2000)
- Huang SM, "Modulation of radiation response and tumor-induced angiogenesis after epidermal growth factor receptor inhibition by ZD1839(Iressa)", Cancer Res, 62(15), pp.4300~4306 (2002)
- Hoffman, RM, "Orthotopic is orthodox : why are orthotopictransplant metastatic models different from all other models?", J. Cell. Biochem, 56, pp.1~3 (1994)
- Myers JN, "An orthotopic nude mouse model of oral squamous cell carcinoma", Clin Cancer Res, 8, pp.293~298 (2002)
- Takashi S, "Lymph node and pulmonary metastasis after transplantation of oral squamous cell carcinoma cell line (HSC-3) into the subcutaneous tissue of nude mouse : detection of metastases by genetic methods using β-globin and mutant p53 genes", Oral Surg Oral Pathol Oral Radiol Endod, 105, pp.486~490 (2008)
- 이은주, "Characterization of newly established oral cancer cell lines derived fromsix squamous cell carcinoma and two mucoepidermoid carcinoma cells", Experimental and Molecular Medicine, 37(5), pp.379~390 (2005), 생화학분자생물학회
- Dinesman A, "Development of a new in vivo model for head and neck cancer", Otolaryngol Head Neck Surg, 103, pp.766~774 (1990)
- Jeffrey N, "An orthotopic nude mouse model of oral tongue squamous cell carcinoma", Clin Cancer Res, 8(1), pp.293~298 (2002)
- 김문기, "누드마우스에서 Snail 발현을 이용한 이종이식 종양의 침윤성 성장 유도", 박사, pp.2005
- Holsinger FC, "Epidermal growth factor receptor blockade potentiates apoptosis mediated by paclitaxel and leads to prolonged survival in an murine model of oral cancer", Clin Cancer Res, 9, pp.9183~9189 (2003)
- Dinney CP, "Isolation and characterization of metastatic variants from human transitional cell carcinoma passaged by orthotopic implantation in athymic nude mice", J Urol, 154, pp.1532~1538 (1995)
- Bruns CJ, "In vivo selection and characterization of metastatic varients from human pancreatic adenocarcinoma by using orthotopic implantation in nude mice", Neoplasia, 1, pp.50~62 (1999)
- Lensch R, "Comprehensive genotype analysis of human prostate cancer cell lines and sublines derived from metastases after orthotopic implantation in nude mice", Int J Oncol, 21, pp.695~706 (2002)
- Boeheim K, "The effect of chemotherapeutic agents on human oral squamous cell carcinoma transplanted to nude mice: a histologic study", Oral Surg Oral Med Oral Pathol, 62(1), pp.50~56 (1986)
- Huang J, "Tumor suppressive effect of retinoid-induced gene- 1(RRIG 1) in esophageal cancer", Cancer Res, 67(4), pp.1589~1593 (2007)
- 박형국, "Making in vivo model to study about human oral cancer", 대한악안면성형재건외과학회지, 19(3), pp.300~310 (1997)
- 박영욱, "마우스에서 타액선암 동위종양 모델 제작을 위한 실험적 연구", 대한구강악안면외과학회지, 33(2), pp.81~93 (2007), 대한구강악안면외과학회
- 장명진, "누드마우스에 이종이식된 구강편평상피세포암종에 대한 "ABNOBAviscum?"의 치료효과에 관한 실험적 연구", 대한악안면성형재건외과학회지, 25(5), pp.383~397 (2003), 대한악안면성형재건외과학회
- 김수곤, "누드마우스에 이종이식된 구강편평상 피세포암종에 대한 thalidomide의 항암효과와 혈관형성억제에 관한 연구", 대한구강악안면외과학회지, 27(4), pp.330~336 (2001)
- 명훈, "구강편평세포암종 이식 누드마우스에서의 혈액 점도 변화", 대한구강악안면외과학회지, 30(2), pp.81~84 (2004), 대한구강악안면외과학회
