구강암 세포주를 이종이식한 설암의 동소위 누드마우스 모델
An orthotopic nude mouse model of tongue carcinoma
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Introduction: Development of carcinoma on oral tongue may cause bilateral cervical lymph node metastasis, rapid invasion and growth of the cancer cells due to rich blood supply in muscle tissues. It is not only difficult to develop an animal experimental model, but also to proceed follow-up research after the development of such model as the induction of cancer lead to difficulty in taking nutrition for the experimental animals that often causes early death. Materials and Methods: IIn this study, author have transplanted YD-10B_(mod) cells into nude mouse oral tongues with different cells number (5×10^4,5×10^5, 5×10^6 cells/mouse) and observed the development aspect of oral tongue cancers. Results: The cancer developed from orthotopic transplantation of YD-10B_(mod) cells into nude mouse oral tongue show invasion and central necrosis of the tumor, similar to the cancers developed human oral tongue cancer. The difference in tumor size and the time of central necrosis development depending on the number of transplanted tumor cells shows the feasibility of extending the survival period of the nude mouse by limiting the transplanted tumor cells to <5×10^4 cells/mouse or under per nude mouse. Conclusion: This nude mouse model could be used effectively in developing effective chemotheray agent and establishing an animal experimental model that can be used to study the mechanism of cervical lymph node metastasis of the oral tongue cancer.
Abstract
Introduction: Development of carcinoma on oral tongue may cause bilateral cervical lymph node metastasis, rapid invasion and growth of the cancer cells due to rich blood supply in muscle tissues. It is not only difficult to develop an animal experimental model, but also to proceed follow-up research after the development of such model as the induction of cancer lead to difficulty in taking nutrition for the experimental animals that often causes early death. Materials and Methods: IIn this study, author have transplanted YD-10B_(mod) cells into nude mouse oral tongues with different cells number (5×10^4,5×10^5, 5×10^6 cells/mouse) and observed the development aspect of oral tongue cancers. Results: The cancer developed from orthotopic transplantation of YD-10B_(mod) cells into nude mouse oral tongue show invasion and central necrosis of the tumor, similar to the cancers developed human oral tongue cancer. The difference in tumor size and the time of central necrosis development depending on the number of transplanted tumor cells shows the feasibility of extending the survival period of the nude mouse by limiting the transplanted tumor cells to <5×10^4 cells/mouse or under per nude mouse. Conclusion: This nude mouse model could be used effectively in developing effective chemotheray agent and establishing an animal experimental model that can be used to study the mechanism of cervical lymph node metastasis of the oral tongue cancer.
참고문헌 (16)
- MJ Kim, "Oral Cancer. 1st ed" (2002), Jisung Publishing
- Zelefsky MJ, "Postoperative radiotherapy for oral cavity cancers: impact of anatomic subsite on treatment outcome", Head Neck, 12, pp.470~475 (1990)
- Salley JJ, "Experimental carcinogenesis in the cheek pouch of the Syrian hamster", J Dent Res, 33, pp.253~262 (1954)
- Morris AL, "Factors influencing experimental carcinogensis in the hamster cheek pouch", J Dent Res, 40, pp.3~15 (1961)
- Hendrickson EA, "The SCID mouse: relevance as an animal model system for studying human disease", Am J Pathol, 143, pp.1511~1522 (1993)
- Kubota T, "Metastatic models of human cancer xenografted in the nude mouse: the importance of orthotopic transplantation", J Cell Biochem, 56, pp.4~8 (1994)
- Killion JJ, "Orthotopic models are necessary to predict therapy of transplantable tumors in mice", Cancer Metastasis Rev, 17, pp.279~284 (1998)
- 이은주, "Characterization of newly established oral cancer cell lines derived fromsix squamous cell carcinoma and two mucoepidermoid carcinoma cells", Experimental and Molecular Medicine, 37(5), pp.379~390 (2005), 생화학분자생물학회
- 김종현, "구강암 세포주의 이소위 누드마우스 종양 모델", 대한구강악안면외과학회지, 35(2), pp.74~82 (2009), 대한구강악안면외과학회
- Johnson N, "Global Epidemiology. In Oral Cancer", pp.7~22 (2003), Martin Dunitz Publication
- Henson B, "An orthotopic floor-of-mouth model for locoregional growth and spread of human squamous cell carcinoma", J Oral Pathol Med, 36, pp.363~370 (2007)
- 장명진, "누드마우스에 이종이식된 구강편평상피세포암종에 대한 ＂ABNOBAviscum？＂의 치료효과에 관한 실험적 연구", 대한악안면성형재건외과학회지, 25(5), pp.383~397 (2003), 대한악안면성형재건외과학회
- Park HK, "Making in vivo model to study about human oral cancer (I)", J Korean Assoc Maxillofac Plast Reconstr Surg, 19, pp.300~310 (1997)
- Qiu C, "A cervical lymph node metastatic model of human tongue carcinoma: Serial and orthotopic transplantation of histologically intact patient specimens in nude mice", J Oral Maxillofac Surg, 61, pp.696~700 (2003)
- Lou E, "Clinical and pathological features of the murine AT-84 orthotopic model of oral cancer", Oral Dis, 9, pp.305~312 (2003)
- Shklar G, "Oral cancer: the diagnosis, therapy, management, and rehabilitation of the oral cancer patient", W.B. Saunders, pp.138~143 (1984)