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Edwardsiella tarda 의 특이 Bacteriophage와 Bacillus subtilis 가 혼합된 사료급이가 나일 틸라피아(Oreochromis niloticus)의 선천적 면역반응과 항균효과에 미치는 영향

The Effects of a Dietary Edwardsiella tarda Specific Bacteriophage and Bacillus subtilis Mixture on Innate Immune Responses and Antibacterial Activity of Nile tilapia Oreochromis niloticus

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초록

The present study investigated the effects of dietary Edwardsiella tarda ( E. tarda ) specific bacteriophage (phage) and Bacillus subtilis ( B. subtilis ) mixture on innate immune responses and antibacterial activity of Nile tilapia, Oreo - chromis niloticus . In a dietary experiment, tilapia were fed the control diet (C), a phage-only supplemented diet (P), a B. subtilis only supplemented diet (B), or a B. subtilis and phage mixed diet (B+P). A respiratory burst and significant increase in lysozyme activity ( P <0.05) were noted in the B+P group, as compared to other groups after 4 days of feed- ing. The B group showed a significant ( P <0.05) increase in respiratory burst and lysozyme activity versus the C and P groups, whereas no significant increases ( P <0.05) were observed in the P and C groups. ACH50 was significantly up-regulated in the B+P group versus other groups after 8 days of feeding ( P <0.05). In vivo antibacterial activity was significantly enhanced in the B+P fed group, as compared to other groups ( P <0.05) after 7 days of E. tarda challenge. A significant ( P <0.05) increase in antibacterial activity was seen in the B group, as compared to C or P groups after 14 days of feeding. These results suggest that a B. subtilis and phage mixture could be utilized as an alternative to antibiotics in the control of fish diseases caused by E. tarda .

Abstract

The present study investigated the effects of dietary Edwardsiella tarda ( E. tarda ) specific bacteriophage (phage) and Bacillus subtilis ( B. subtilis ) mixture on innate immune responses and antibacterial activity of Nile tilapia, Oreo - chromis niloticus . In a dietary experiment, tilapia were fed the control diet (C), a phage-only supplemented diet (P), a B. subtilis only supplemented diet (B), or a B. subtilis and phage mixed diet (B+P). A respiratory burst and significant increase in lysozyme activity ( P <0.05) were noted in the B+P group, as compared to other groups after 4 days of feed- ing. The B group showed a significant ( P <0.05) increase in respiratory burst and lysozyme activity versus the C and P groups, whereas no significant increases ( P <0.05) were observed in the P and C groups. ACH50 was significantly up-regulated in the B+P group versus other groups after 8 days of feeding ( P <0.05). In vivo antibacterial activity was significantly enhanced in the B+P fed group, as compared to other groups ( P <0.05) after 7 days of E. tarda challenge. A significant ( P <0.05) increase in antibacterial activity was seen in the B group, as compared to C or P groups after 14 days of feeding. These results suggest that a B. subtilis and phage mixture could be utilized as an alternative to antibiotics in the control of fish diseases caused by E. tarda .

참고문헌 (51)

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관련 논문AI추천

Edwardsiella tarda의 특이 Bacteriophage와 Bacillus subtilis KM-1혼합액이 Edwardsiella tarda 에 미치는 항균효과백민석, 최상훈, 황요셉수산생명의학회 · 한국어병학회지2013 · 제26권 제3호The dietary effect of medicinal herbs extract and multiple probiotics mixture on the growth performance, innate immune response and antibacterial activity of nile tilapia Oreochromis niloticus강인성, 강태윤, 박관하, 방석진, 전세영, 정상목, 최상훈, 최재혁, 황요셉수산생명의학회 · 한국어병학회지2019 · 제32권 제1호서해안 양식장에서 분리한 Edwadsiella tarda의 특이 bacteriophage 특성강자영, 경서봉, 백민석, 이정은, 이찬흔, 최상훈, 한미정, 허용주수산생명의학회 · 한국어병학회지2011 · 제24권 제2호Effects of Bacillus SW1-1 coated diets on innate immunity and disease resistance of olive flounder Paralichthys olivaceus against Edwardsiella tarda infection김민기, 이경준, Buddi E. Gunathilaka, Sungho Lee, Youjeong Kim한국수산과학회 · Fisheries and Aquatic Sciences2022 · 제25권 제4호사료 내 Bacillus 균주의 첨가가 넙치(Paralichthys olivaceus) 치어의 성장, 사료효율, 선천성면역 및 연쇄구균 질병저항성에 미치는 영향송진우, 양시용, 오대한, 우서형, 이경준, 차지훈한국수산과학회 · 한국수산과학회지2012 · 제45권 제1호Respiratory burst activity of tilapia (Oreochromis mosambicus) phagocytes to Edwardsiella tarda ghosts권세련, 김기홍, 김민선, 남윤권, 이은혜수산생명의학회 · 한국어병학회지2006 · 제19권 제3호

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