서해안 양식장에서 분리한 Edwadsiella tarda의 특이 bacteriophage 특성
Characterization of Edwardsiella tarda specific phage isolated from fish farms on west coast of Korea
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Edwardsiella tarda is a broadly infectious agent against freshwater and seawater fishes. In the present study, E.tarda specific phage was isolated from fish farms on the west coast of Korea, and the effect of environmental factors such as pH and water temperature on the phage activity was investgated. As a fish model, Nile tilapia (Oreochromis niloticus) was used and the interaction between E. tarda and phage was investigated. The phage activity, in pH test, appeared even higher in seawater than freshwater and was evenly constant up to 50℃. The phage and E. tarda were inoculated in tilapia and the phage activity and E. tarda viability were checked on the time intervals. As a result, the number of E. tarda in the group of E. tarda plus phage was constantly reduced to 24 hr post-inoculation compared to the control group without phage, whereas the phage activity was slightly increased in the experiment group. The results suggest that it might be possible to use phage to control the fish disease caused by E. tarda.
Abstract
Edwardsiella tarda is a broadly infectious agent against freshwater and seawater fishes. In the present study, E.tarda specific phage was isolated from fish farms on the west coast of Korea, and the effect of environmental factors such as pH and water temperature on the phage activity was investgated. As a fish model, Nile tilapia (Oreochromis niloticus) was used and the interaction between E. tarda and phage was investigated. The phage activity, in pH test, appeared even higher in seawater than freshwater and was evenly constant up to 50℃. The phage and E. tarda were inoculated in tilapia and the phage activity and E. tarda viability were checked on the time intervals. As a result, the number of E. tarda in the group of E. tarda plus phage was constantly reduced to 24 hr post-inoculation compared to the control group without phage, whereas the phage activity was slightly increased in the experiment group. The results suggest that it might be possible to use phage to control the fish disease caused by E. tarda.
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