방사선에 의한 난포 퇴축과 난포 대식세포에 관한 형태학적 연구
Morphological Study on the Radiation-induced Follicular Atresia and the Follicular Macrophage
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목적 : 방사선이 난포 퇴축을 일으키는 난포과립층세포들에 미치는 영향과 난포내 대식세포의 일부역할을 형태학적으로 살펴보기 위해 본 연구를 수행하였다.연구 방법 : 3주령의 Sprague-Dawley계 흰쥐를 실험동물로 8 Gy의 방사선을 조사하고, 조사 후 6시간, 12시간 및 24시간에 각각 희생하여 난소를 채취하고 통상적인 방법으로 처리한 후 광학현미경적 및 투과전자현미경적 관찰, TUNEL 및 대식세포 면역조직화학을 실시하였다.결과 : 1) 방사선 조사 후 6시간에 난포 퇴축이 대조군에 비해 유의하게 (p<0.01) 증가하였으며 12시간과 24시간 후에는 감소하였다. 2) 방사선은 난포과립층세포들에서 염색질의 농축, 핵분체 형성 등 전형적인 아포토시스를 유발하였다. 3) 아포토시스를 일으킨 과립층세포들은 주변의 정상적인 과립층세포들과 대식세포들에 의해 탐식되는 것을 관찰할 수 있었다. 4) 퇴축이 진행되지 않는 일부 난포에서 방사선 저항성이 있는 과립층세포들을 관찰할 수 있었다. 5) 난포내에서 대식세포는 과립층과 난포동 모두에서 관찰되었으며, 탐식작용에 관여하지 않는 것으로 생각되는 대식세포가 난포기저막 위에서 관찰되었다.
Abstract
Objective : It is well known that X-ray induces follicular atresia, but the exact mechanism of atresia is not still unveiled completely. In addition, the role of macrophage related with clean-up the dead granulosa cells and other functions within the ovarian follicle is emphasized recently. The aim of this study is to assess the radiation-induced morphological changes of ovarian follicles and follicular macrophages. Methods : 8 Gy X-ray irradiated on the 3-week old rats (Sprague-Dawley strain), sacrificed at 6, 12, and 24 hours after irradiation, and performed morphological studies with light and transmission electron microscopy, TUNEL, and macrophage immunohistochemistry. Results : Follicular atresia increased significantly (p<0.01) at 6 hours after X-irradiation, and it was decreased significantly (p<0.01) at 12 and 24 hours after irradiation. X-ray induced chromatin condensation in the nucleus and nuclear fragmentation of granulosa cells, which were the typical features of apoptosis. Apoptotic granulosa cells were phagocytosed by the neighboring normal granulosa cells and the macrophages. During atresia of follicles, radioresistant granulosa cells were found in some follicles, which showed similar features morphologically with the granulosa cells of normal follicles. Macrophages were found both within the antrum and at the follicular granulosa layer. Conclusion : X-radiation induced follicular atresia by means of granulosa cell apoptosis, and radioresistant granulosa cells which have similar features morphologically with the granulosa cells of normal follicles were observed in some follicles. And the macrophages which phagocytose the apoptotic granulosa cells were located within the follicular antrum and at the follicular granulosa layer.
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