자궁내막 Heparin-Binding EGF-like Growth Factor (HB-EGF)의 발현에 미치는 외인성 성선자극호르몬의 작용
Effect of exogenous gonadotropin on HB-EGF expression in endometrium
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목적: 체외수정시술시 성선자극호르몬은 자궁수용성에 부정적인 영향을 주는 것으로 생각되어 왔으며, HB-EGF는 생쥐 자궁내막에서 착상기에서 중요한 역할을 하는 것으로 시사되어 왔다. 본 연구에서는 생쥐 자궁내막에서 착상 직전 발현되는 HB-EGF의 발현양상이 과배란유도에 의하여 어떠한 영향을 받는지에 대하여 평가하였다. 연구 방법: 위약을 투여한 자연임신군과 성선자극호르몬 PMSG 5, 10 IU를 투여한 군에서 임신 5.5일의 자궁을 이용하여 그 발현정도의 비교, 분석을 시행하였다. 각 mRNA 발현의 평가에는 competitive RT-PCR을 이용하였다. 결과: HB-EGF mRNA 발현은 internal standard의 10-4에서 10-6의 희석농도에서 관찰되었으며, dual band (423/360 bp)는 10-5 희석농도에서 관찰되었다. 임신 제 5.5일의 생쥐자궁에서 HB-EGF mRNA의 발현은 PMSG 투여군에서 자연임신군에 비하여 차이를 나타내지 아니하였다. 또한 PMSG의 용량에 따른 차이는 발견할 수 없었다. 결론: 성선자극호르몬을 이용한 과배란유도는 생쥐 자궁내막에서 HB-EGF 발현에 영향을 미치지 아니한다. 향후 과배란유도에 의하여 영향을 받는 다른 인자들이 착상의 어느 단계에서 영향을 미치는지에 대한 연구가 필요할 것으로 사료된다.
Abstract
Objective: Gonadotropin hyperstimulation for in vitro fertilization and embryo transfer (IVF-ET) has been considered to be harmful to the uterine receptivity. The role of HB-EGF has been implicated in the mouse embryonic implantation. In the present study, we assessed the effect of gonadotropin hyperstimulation on the expression of HB-EGF mRNA in murine endometrium. Methods: Competitive, reverse transcription-PCR was performed to evaluate the expression of HB-EGF mRNA in the hyperstimulated murine endometrum from pregnant uteri of gestation day (g.d.) 5.5 after the injection of saline (placebo), pregnant mare serum gonadotropin (PMSG) 5 IU and 10 IU. Results: HB-EGF expression was observed at 10-4-10-6 dilution concentrations of internal standard, and dual bands (423/360 bp) were observed at 10-5 dilution concentration. The expression of HB-EGF mRNA showed no significant change in the endometrial expression in PMSG groups, compared to the control group, on g.d. 5.5. There was no dose-response relationship between the two PMSG groups. Conclusion: Our data reveals that ovarian hyperstimulation using gonadotropin does not influence the expression of HB-EGF in the murine endometrium. Further studies are necessary to elucidate which stage of embryonic implantation and the expression of other substance is affected by the ovarian hyperstimulation.
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