초기 염류 스트레스 반응 인삼 잎 단백질체 분석
Proteomics Analysis of Early Salt-Responsive Proteins in Ginseng (Panax ginseng C. A. Meyer) Leaves
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초록
Salt stress is one of the major abiotic stresses affecting the yield of ginseng (Panax ginseng C. A. Meyer). Theobjective of this study was to identify bio-marker, which is early responsive in salt stress in ginseng, using proteomicsapproach. Ginseng plants were exposed to 5 ds/m salt concentration and samples were harvested at 0, 6, 12 and 18 hoursafter exposure. Total proteins were extracted from ginseng leaves treated with salt stress using Mg/NP-40 buffer and wereseparated on high resolution 2-DE. Approximately 1003 ± 240 (0 h), 992 ± 166 (6 h), 1051 ± 51 (12 h) and 990 ± 160 (18 h)spots were detected in colloidal CBB stained 2D maps. Among these, 8 spots were differentially expressed and were identifiedby using MALDI-TOF/TOF MS or/and LC-MS/MS. Ethylene response sensor-1 (spot GL 1), nucleotide binding protein(spot GL 2), carbonic anhydrase-1 (spot GL 3), thylakoid lumenal 17.9 kDa protein (spot GL 4) and Chlorophyll a/b bindingprotein (spot GL 5, GL 6) were up-regulated at the 12 and 18 hour, while RuBisCO activase B (spot GL 7) and DNA helicase(spot GL 8) were down-
Abstract
Salt stress is one of the major abiotic stresses affecting the yield of ginseng (Panax ginseng C. A. Meyer). Theobjective of this study was to identify bio-marker, which is early responsive in salt stress in ginseng, using proteomicsapproach. Ginseng plants were exposed to 5 ds/m salt concentration and samples were harvested at 0, 6, 12 and 18 hoursafter exposure. Total proteins were extracted from ginseng leaves treated with salt stress using Mg/NP-40 buffer and wereseparated on high resolution 2-DE. Approximately 1003 ± 240 (0 h), 992 ± 166 (6 h), 1051 ± 51 (12 h) and 990 ± 160 (18 h)spots were detected in colloidal CBB stained 2D maps. Among these, 8 spots were differentially expressed and were identifiedby using MALDI-TOF/TOF MS or/and LC-MS/MS. Ethylene response sensor-1 (spot GL 1), nucleotide binding protein(spot GL 2), carbonic anhydrase-1 (spot GL 3), thylakoid lumenal 17.9 kDa protein (spot GL 4) and Chlorophyll a/b bindingprotein (spot GL 5, GL 6) were up-regulated at the 12 and 18 hour, while RuBisCO activase B (spot GL 7) and DNA helicase(spot GL 8) were down-
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