본문 바로가기
KERD
논문

산벚나무 수피추출물의 생리활성 및 페놀성 화합물 분리

Biological Activity Investigation, and Phenol Compounds Isolation from Barks of Prunus Sargentii R.

이진영|김태훈|권오준|박근혜|박강수|박태순|안봉전|박정미|조영제|최경임
한국약용작물학회|2008.06|한국약용작물학회지 (KOREAN JOURNAL OF MEDICINAL CROP SCIENCE)|제16권 제3호|pp. 173-182

1

조회

0

다운로드

7

인용

0

참고문헌

키워드

TaxifolinPrunus sargentii R. Biological activitiesEpicatechin

초록

Prunus sargentii of Rosaceae familiy, has been reported to have radical scavenging activity and anti-inflammatory effect. On these facts, this research was conducted to evaluate pharmaceutical activities of the bark extracts P. sargentii. Free radical scavenging activities of fraction (Fr) −5~10 isolated from P. sargentii was higher than 80% respectively at 10ppm. The superoxide dismutase (SOD)-like activity of Fr-5, 9 were about 97, 84%, respectively at 1,000 ppm. Xanthine oxidase inhibitory effect of Fr-9, 10 were about 75, 78%, respectively at 1,000 ppm. The tyrosinase inhibitory effect related to skin-whitening was 72, 68% in Fr-2, 9 isolated from Prunus sargentii R. at 1,000 ppm. Hyaluronidase inhibition activity related to the anti-inflammation effect was 98% for Fr-8 at 500 ppm. Isolation of the methanol soluble fraction from P. sargentii yielded two major phenol compounds, (−)-epicatechin and taxifolin. The structure of the compound was certainly determined by chemical analyses, as well as NMR and Mass spectroscopy. The present study was carried out in a search for new cosmetic material from the bark from P. sargentii. and, (−)-epicatechin and taxifolin were isolated as active principles. So P. sargentii R. methanolic extracts may be used for the cosmetic material.

Abstract

Prunus sargentii of Rosaceae familiy, has been reported to have radical scavenging activity and anti-inflammatory effect. On these facts, this research was conducted to evaluate pharmaceutical activities of the bark extracts P. sargentii. Free radical scavenging activities of fraction (Fr) −5~10 isolated from P. sargentii was higher than 80% respectively at 10ppm. The superoxide dismutase (SOD)-like activity of Fr-5, 9 were about 97, 84%, respectively at 1,000 ppm. Xanthine oxidase inhibitory effect of Fr-9, 10 were about 75, 78%, respectively at 1,000 ppm. The tyrosinase inhibitory effect related to skin-whitening was 72, 68% in Fr-2, 9 isolated from Prunus sargentii R. at 1,000 ppm. Hyaluronidase inhibition activity related to the anti-inflammation effect was 98% for Fr-8 at 500 ppm. Isolation of the methanol soluble fraction from P. sargentii yielded two major phenol compounds, (−)-epicatechin and taxifolin. The structure of the compound was certainly determined by chemical analyses, as well as NMR and Mass spectroscopy. The present study was carried out in a search for new cosmetic material from the bark from P. sargentii. and, (−)-epicatechin and taxifolin were isolated as active principles. So P. sargentii R. methanolic extracts may be used for the cosmetic material.

참고문헌 (48)

  1. An BJ, "Cosmetic and biological novel materials", pp.41~69 (1999), Budagaya Press. Seoul
  2. 안봉전, "黃連의 생리활성검증 및 화장품 소재로서의 이용가능성", 대한본초학회지, 20(3), pp.83~92 (2005), 대한본초학회
  3. An BJ, "Phytochemical", pp.15~23 (2006), Sunmeongsa Press. Seoul.
  4. Association of Analytical Chemists, "Offical methods of analysis of the AOAC. 14th ed" (1984), Washington (DC) : Association of Analytical Chemists INC.
  5. Blois MS, "Antioxidant determination by the use of a stable free radical", Nature, 26, pp.1199~1120 (1958)
  6. Carmichael J, "Evaluation of a tetrazolium based semiautomated colorimetric assay: assessment of chemosensitivity testing", Cancer Res, 47, pp.936~942 (1987)
  7. 차배천, "야생복숭아 나무 추출물의 항산화 및 항염증 효과", 한국약용작물학회지, 12(4), pp.289~294 (2004), 한국약용작물학회
  8. 차배천, "계혈등의 항산화 활성", 생약학회지, 36(1), pp.50~55 (2005), 한국생약학회
  9. Chae YM, "Chinese medicinal dictionary", pp.260~260 (1996), Chinese medicine Press. Beijing.
  10. Cho EJ, "Study on the inhibitory effects of Korean medicinal plants and their main compounds in the 1,1-diphenyl-2- picrylhydrazyl radical", Phytomedicin, 10, pp.544~544 (2003)
  11. Cho MJ, "Nitrite scavenging ability and SOD-like activity of a sterol glucoside from Chrysanthemum coronarium L. var. spatiosum", Kor. J. Food Sci. Technal, 39, pp.77~82 (2007)
  12. Cho YC, "Isolation and enzyme inhibition of tannins from Korean Green Tea", Kor. J. Biochem, 26, pp.216~223 (1993)
  13. Choi J, "Anticiceptive and anti-inflammatory effects of Nigaichigoside F1 and 23-hydroxytormentic acid obtained from Rubus coreanus", Biol. Pharm. Bull, 26, pp.1436~1436 (2003)
  14. Do JC, "Studies on the constituents of the roots of Rubus parvifolius (I). Isolation of (−)-epicatechin", Kor. J. Pharmacogn, 19, pp.170~173 (1988)
  15. Gao H, "Antitumor-promoting constituents from Chaenomeles sinensis K. and their activities in JB6 mouse epidermal cells", Chem. Pharm. Ball, 51, pp.1318~1318 (2003)
  16. Han BH, "Immuno-suppressant activity of cherry bark extract", J. Pharmacog Kor, 9, pp.173~175 (1978)
  17. Hayashi T, "Inhibition of cow's milk xanthine oxidase by flavonoids", J. Nat Prod, 51, pp.345~348 (1988)
  18. Jin GS, "A botanical list", pp.1680~1681 (1992), Hakwon Press. Beijing
  19. Ju YC, "The northeast medicinal plant", pp.212~214 (1989), Amur river scientific Press. Beijing
  20. Jung S, "Genome database for Rosaceae (GDR) integrated web resources for Rosaceae genomics and genetics research", BMC Bioinformatics, 5, pp.130~130 (2004)
  21. Jung SW, "Screening of tyrosinase inhibitor from plants", Kor J. Food Sci. Technol, 27, pp.891~896 (1995)
  22. Kang YH, "The nitrite scavenging and electron donating ability of phenolic compounds", Kor J Food Sci Technol, 28, pp.232~232 (1996)
  23. Pharmaceutical Botany, "Kam WS", A national Chinese medicinne institute. Seoul., 305~306 (1981)
  24. Kim HK, "Antioxidative activity and physiological activity of some Korean medical plants", Kor. J. Food Sci. Technol, 27, pp.80~85 (1995)
  25. Kim JG, "The original color nature medicines dictionary", pp.160~160 (1984), Namsandang Press. Seoul
  26. Kim JG, "A primary color pictorial book of the orient nature medicines", pp.165~165 (1995), Yeonglimsa. Seoul.
  27. Kim JO, "In Illustrated Natural Drugs Encyclopedia (Color Edition)", pp.437~437 (2005), Yeokang Press. Seoul
  28. Kim KS, "New material isolation and structural elucidation", pp.193~251 (2003), Chosun Univ. Press. Korea.
  29. Kim YJ, "Medicinal resources botany", pp.136~136 (1974), Dongmangsa Press. Seoul
  30. Kim YM, "Oxyresveratrol and hydroxystibene compounds, inhibitory effect on tyrosinase and mechanism of action", J. Biol. Chem, 277, pp.163~164 (2002)
  31. Lee HJ, "Studies on biological activity of wood extractives (VI)-Flavonoids in heartwood of Prunus sargentii", Mokchae Konghak, 29, pp.133~139 (2001)
  32. 이학주, "수목추출물의 생리활성에 관한 연구(XII)- 산벚나무 심재 추출성분의 항균 및 항산화활성(2) -", 목재공학, 31(4), pp.16~23 (2003), 한국목재공학회
  33. Yeon-Suk Lee, "Antioxidative Constituents from the Woods of Liriodendron tulipifera", 목재공학, 32(6), pp.43~49 (2004), 한국목재공학회
  34. Lee JT, "study on the materials of functional Korea herb cosmetics using atractylodis rhizoma alba and saururi herba seu radix", J. applied oriental medicine, 5, pp.7~14 (2005)
  35. Lee SJ, "Korea folk medicine", pp.116~116 (1976), Seomoondang Press. Seoul
  36. Lee SS, "Studies on biological activity of wood extractives(7)-Antimicrobial and antioxidation activities of extractives from the heartwood of Prunus sargentii", Mokchae Konghak, 29, pp.140~145 (2001)
  37. Ligaa U, "A pictorial book of the medicinal plant", pp.22~23 (1996), Korean society of Chunal manual medicine. Seoul
  38. 임정대, "한국산 약용식물로부터의 SOD 활성 및 Phenolic Compounds 함량 비교", 한국약용작물학회지, 12(3), pp.191~202 (2004), 한국약용작물학회
  39. Marklund S, "Marklund S, Marklund G (1974) Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase", Eur. J. Biochem, 47, pp.469~474 (1974)
  40. Matsuda H, "Studies of cuticle drugs from natural sources II. Inhibitory effects of prunus plants on melamin biosynthesis", Biol. Pharm. Bull, 17, pp.1417~1417 (1994)
  41. Oku H, "Antipruritic effects of the fruits of Chaenomeles sinensis", Biol. Pharm. Bull, 26, pp.1031~1031 (2003)
  42. Park SJ, "Species identification of Tripitaka Koreana", Mokchae Konghak, 24, pp.80~89 (1996)
  43. Reissig JL, "A modified colorimetric method for the estimation of N-acetylamino sugars", J. Biol. Chem, 217, pp.959~966 (1955)
  44. Stirpe F, "The regulation of rat liver xanthine oxidase. Conversion in vitroof the enzyme activity from dehydrogenase (type D) to oxidase (type O)", J. Biol. Chem, 244, pp.3855~3863 (1969)
  45. Wieslaw Oleszek, "Flavonoids from Pinus sylvestris needles and their variation in trees of different origin grown for nearly a century at the same area", Biochemical systematics and ecology, 30, pp.1011~1022 (2002)
  46. 허영남, "풀명자 뿌리의 성분", 생약학회지, 33(4), pp.267~271 (2002), 한국생약학회
  47. Yagi A, "The effect of tyrosinase inhibition for aloe", Planta Medica, 3981, pp.517~519 (1986)
  48. Yeo SG, "Inhibition of xanthine oxidase by tea extracts from green tea, oolong tea and black tea", J. Kor. Soc. Food Nutr, 24, pp.154~159 (1995)

관련 논문AI추천

산벚나무 부위별 추출물의 생리활성 비교김선민, 양선아, 이경인, 조주현, 표병식한국약용작물학회 · 한국약용작물학회지2012 · 제20권 제3호벚나무 잎 과 어린 가지 추출물의 항산화 활성효과 연구김성건, 김숙하, 한효선, 황완균한국피부과학연구원 · 아시안뷰티화장품학술지2010 · 제8권 제2호Whitening and Anti-oxidative Activities of Chemical Components Extracted from Branches of Sorbus alnifolia김정은, 이남호, Bo Shi Liu대한화학회 · 대한화학회지2023 · 제67권 제2호초고압 및 초음파 추출공정을 이용한 매자나무 수피의 향장활성 증진김영옥, 김지선, 김진철, 서용창, 이현용, 차선우, 최운용, 하지혜, JIN LING한국약용작물학회 · 한국약용작물학회지2011 · 제19권 제1호산벚나무 가지 추출물 및 용매 분획물의 Nitric Oxide 생성 억제 효과와 여드름 원인균에 대한 항균활성김선민, 양선아, 표병식한국약용작물학회 · 한국약용작물학회지2016 · 제24권 제2호음나무(Kalopanax pictus) 수피 추출물의 항산화 활성에 관한 연구김종봉, 이양숙, 최정숙, 황중덕대한미용학회 · 대한미용학회지2011 · 제7권 제4호

이 간행물의 다른 논문 (10건)

짚신나물, 삼백초의 항산화와 항암활성 효과정봉환, 서훈석, 조용구pp. 139-143인산과 카리 시비량에 따른 바위솔의 생장과 개화김홍영, 신성철, 강진호, 이창우, 전승호pp. 144-149유통생약 황금의 품질모니터링 연구이아영, 이혜원, 장설, 김홍준, 김호경, 추병길, 천진민pp. 150-154Leuconostoc fallax LH3이 생산하는 효소에 의한 Ginsenoside Rd의 Ginsenoside F2로의 전환성락금, 전림호, 양덕춘, 김세화, 김명겸, 박민주, 김호빈, 나주련pp. 155-160한국 약용식물 33분류군의 염색체수와 핵형분석김찬수, 김건래, 김수영, 박상홍, 이중구, 장태수, 이원규, 김진기pp. 161-167
원문 보기