Optimization of Metarhizium koreanum MN031-Mt 46: Nutritional Supplementation to Improve Conidia and Cuticle-Degrading Enzyme Production by Solid-State Fermentation
1
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
This study aimed to evaluate five different mixed agricultural wastes as potential substrates for solid-state fermentation (SSF) to produce conidia of Metarhizium koreanum MN031-Mt 46. Singlefactor experiments and a Box-Behnken design (BBD) were employed to optimize the fermentation conditions for enhanced conidia yield. Results indicated that a mixed substrate comprising broken rice and rice bran significantly enhanced the optimal production of aerial conidia of MN031-Mt 46. Optimal fermentation conditions established through response surface methodology (RSM) revealed that with the addition of shrimp shell waste to the mixed substrate, conidia production increased to 8.45 × 108 conidia per gram of dry substrate at 26.19o C temperature, 39.76% moisture, and 1.45% of shrimp shell waste after 301.87 h of incubation. Enhanced conidia performance indices were observed, including higher conidia weight, increased water content, and reduced residue post-harvest. The optimized fermentation conditions resulted in enhanced cuticle-degrading enzymatic activities, with maximum activities of 58.78 ± 2.29 U g- ¹ ds for protease, 126.57 ± 6.47 U g- ¹ ds for lipase, and 58.32 ± 0.78 U g- ¹ ds for chitinase. These findings highlight the potential and versatility of mixed SSF using cost-effective agricultural waste for biopesticide and hydrolytic enzyme production, while promoting sustainable waste management and environmental pollution control, aligning with circular economy principles.
Abstract
This study aimed to evaluate five different mixed agricultural wastes as potential substrates for solid-state fermentation (SSF) to produce conidia of Metarhizium koreanum MN031-Mt 46. Singlefactor experiments and a Box-Behnken design (BBD) were employed to optimize the fermentation conditions for enhanced conidia yield. Results indicated that a mixed substrate comprising broken rice and rice bran significantly enhanced the optimal production of aerial conidia of MN031-Mt 46. Optimal fermentation conditions established through response surface methodology (RSM) revealed that with the addition of shrimp shell waste to the mixed substrate, conidia production increased to 8.45 × 108 conidia per gram of dry substrate at 26.19o C temperature, 39.76% moisture, and 1.45% of shrimp shell waste after 301.87 h of incubation. Enhanced conidia performance indices were observed, including higher conidia weight, increased water content, and reduced residue post-harvest. The optimized fermentation conditions resulted in enhanced cuticle-degrading enzymatic activities, with maximum activities of 58.78 ± 2.29 U g- ¹ ds for protease, 126.57 ± 6.47 U g- ¹ ds for lipase, and 58.32 ± 0.78 U g- ¹ ds for chitinase. These findings highlight the potential and versatility of mixed SSF using cost-effective agricultural waste for biopesticide and hydrolytic enzyme production, while promoting sustainable waste management and environmental pollution control, aligning with circular economy principles.