Application and Analysis of Rhizopus oryzae Mycelia Extending Characteristic in Solid-state Fermentation for Producing Glucoamylase
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Enhanced application of solid-state fermentation (SSF) in industrial production and the influence of SSF of Rhizopus K1 on glucoamylase productivity were analyzed using the flat band method. A growth model was implemented through SSF of Rhizopus K1 in this experiment, and spectrophotometric method was used to determine glucoamylase activity. Results showed that in bran and potato culture medium with 70% moisture in a loose state, μ of mycelium reached to 0.15 h−1 after 45 h of culture in a thermostatic water bath incubator at 30°C. Under a low-magnification microscope, mycelial cells appeared uniform, bulky with numerous branches, and were not easily ruptured. The generated glucoamylase activity reached to 55 U/g (dry basis). This study has good utilization value for glucoamylase production by Rhizopus in SSF.
Abstract
Enhanced application of solid-state fermentation (SSF) in industrial production and the influence of SSF of Rhizopus K1 on glucoamylase productivity were analyzed using the flat band method. A growth model was implemented through SSF of Rhizopus K1 in this experiment, and spectrophotometric method was used to determine glucoamylase activity. Results showed that in bran and potato culture medium with 70% moisture in a loose state, μ of mycelium reached to 0.15 h−1 after 45 h of culture in a thermostatic water bath incubator at 30°C. Under a low-magnification microscope, mycelial cells appeared uniform, bulky with numerous branches, and were not easily ruptured. The generated glucoamylase activity reached to 55 U/g (dry basis). This study has good utilization value for glucoamylase production by Rhizopus in SSF.
참고문헌 (50)
- Galliano H, "Lignin biodegradation by cultures of Rigidoporus lignosus in solid state conditions", FEMS Microbiol. Lett, 67, pp.295~299 (1990)
- Vong WC, "Solid-state fermentation with Rhizopus oligosporus and Yarrowia lipolytica improved nutritional and flavour properties of okara", LWT-Food. Sci. Technol, 90, pp.316~322 (2018)
- Pathania S, "Utilization of horticultural waste (Apple Pomace) for multiple carbohydrase production from Rhizopus delemar F2 under solid state fermentation", J. Genet. Eng. Biotechnol, 16, pp.181~189 (2018)
- Saito K, "Production of lactic acid from xylose and wheat straw by Rhizopus oryzae", J. Biosci. Bioeng, 114, pp.166~169 (2012)
- Lennartsson PR, "Encyclopedia of Food Microbiology", pp.284~290 (2014), Academic Press
- Londoño-Hernández L, "Rhizopus oryzae – Ancient microbial resource with importance in modern food industry", Int. J. Food Microbiol, 257, pp.110~127 (2017)
- Shiraga S, "Construction of the combinatorial library of Rhizopus oryzae lipase mutated in the lid domain by displaying on yeast cell surface", J. Mol. Catal. B-Enzym, 17, pp.167~173 (2002)
- Nishise H, "Production of raw cassava starch-digestive glucoamylase by Rhizopus sp. in liquid culture", J. Ferment. Technol, 66, pp.397~402 (1988)
- Ozturkoglu-Budak S, "Protease and lipase activities of fungal and bacterial strains derived from an artisanal raw ewe’s milk cheese", Int. J. Food Microbiol, 237, pp.17~27 (2016)
- Aikat K, "Protease production in solid state fermentation with liquid medium recycling in a stacked plate reactor and in a packed bed reactor by a local strain of Rhizopus oryzae", Process. Biochem, 36, pp.1059~1068 (2001)
- Canet A, "Exploring substrate specificities of a recombinant Rhizopus oryzae lipase in biodiesel synthesis", New Biotechnol, 39(Part A), pp.59~67 (2017)
- Pashangeh K, "Biochemical characterization and stability assessment of Rhizopus oryzae lipase covalently immobilized on aminofunctionalized magnetic nanoparticles", Int. J. Biol. Macromol, 105(Part 1), pp.300~307 (2017)
- Goldberg I, "Organic acids: old metabolites, new themes", J. Chem. Technol. Biotechnol, 81, pp.1601~1611 (2006)
- Yang ST, "Comprehensive Biotechnology", pp.63~177 (2011), Elsevier
- Wang C-Y, "l-Lactic acid fermentation by culture of Rhizopus oryzae using ammonia as neutralizing agent", J. Taiwan Inst. Chem. E, 45, pp.1~5 (2014)
- Zheng Y, "Using tobacco waste extract in pre-culture medium to improve xylose utilization for l-lactic acid production from cellulosic waste by Rhizopus oryzae", Bioresour. Technol, 218, pp.344~350 (2016)
- Machado de Castro A, "Current Developments in Biotechnology and Bioengineering", pp.185~210 (2018), Elsevier
- Schutyser MAI, "Substrate aggregation due to aerial hyphae during discontinuously mixed solid-state fermentation with Aspergillus oryzae: Experiments and modeling", Biotechnol. Bioeng, 83, pp.503~513 (2003)
- Sugai-Guérios MH, "Colonization of solid particles by Rhizopus oligosporus and Aspergillus oryzae in solid-state fermentation involves two types of penetrative hyphae: a model-based study on how these hyphae grow", Biochem. Eng. J, 114, pp.173~182 (2016)
- Xu Q, "Integrated transcriptomic and metabolomic analysis of Rhizopus oryzae with different morphologies", Process Biochem, 64, pp.74~82 (2018)
- Costa JAV, "Current Developments in Biotechnology and Bioengineering", pp.1~17 (2018), Elsevier
- Vaseghi Z, "Production of active lipase by Rhizopus oryzae from sugarcane bagasse: solid state fermentation in a tray bioreactor", Int. J. Food Sci. Technol, 48, pp.283~289 (2013)
- Stertz SC, "Growth kinetics of Rhizopus formosa MUCL 28422 on raw cassava flour in solid state fermentation", J. Chem. Technol. Biot, 58, pp.580~586 (1999)
- Zhao H-M, "Impact of solid state fermentation on nutritional, physical and flavor properties of wheat bran", Food Chem, 217, pp.28~36 (2017)
- Varzakas TH, "Glucoamylases production of Aspergillus niger in solid state fermentation using a continuous counter-current reactor", Int. J. Food. Sci. Technol, 43, pp.1159~1168 (2008)
- Wickens GE., "Economic Botany: Principles and Practices", pp.151~207 (2001), Springer Netherlands
- de Andrade Silva CA, "Physiology of Lichtheimia ramosa obtained by solidstate bioprocess using fruit wastes as substrate", Bioproc. Biosyst. Eng, 37, pp.727~734 (2014)
- Singh SK, "Current Developments in Solid-state Fermentation", pp.183~204 (2008), Springer
- Shimada Y, "Purification of ethyl docosahexaenoate by selective alcoholysis of fatty acid ethyl esters with immobilized Rhizomucor miehei lipase", J. Am. Oil. Chem. Soc, 75, pp.1565~1571 (1998)
- Coradin JH, "A three-dimensional discrete latticebased system for modeling the growth of aerial hyphae of filamentous fungi on solid surfaces: A tool for investigating micro-scale phenomena in solid-state fermentation", Biochem. Eng. J, 54, pp.164~171 (2011)
- Yoon LW, "Fungal solid-state fermentation and various methods of enhancement in cellulase production", Biomass. Bioenerg, 67, pp.319~338 (2014)
- Ibarruri J, "Rhizopus oryzae as Fermentation Agent in Food Derived Sub-products", Waste. Biomass. Valori, 9, pp.2107~2115 (2017)
- Boswell GP, "Growth and function of fungal mycelia in heterogeneous environments", B. Math Biol, 65, pp.447~477 (2003)
- Singhania RR, "Recent advances in solid-state fermentation", Biochem. Eng. J, 44, pp.13~18 (2009)
- Soccol CR, "Recent developments and innovations in solid state fermentation", Biotech. Res. Innov, 1, pp.52~71 (2017)
- Chisti Y., "Industrial Biotechnology", pp.149~171 (2010), Wiley-VCH Verlag GmbH & Co. KGaA
- Chisti Y., "Encyclopedia of Bioprocess Technology", pp.2446~2462 (1999), Wiley
- Chisti Y., "Encyclopedia of Industrial Biotechnology", pp.4516~4534 (2010), Wiley
- Kong W, "Isolation of glucoamylase-producing rhizopus strains and study on their enzyme properties", Liquor-Making Sci. Technol, 219, pp.32~35 (2012)
- Díaz BHC, "Morphological characterization of the growing front of Rhizopus oligosporus in solid media", J. Food Eng, 101, pp.309~317 (2010)
- Martinez-Ruiz A, "Continuous ethyl oleate synthesis by lipases produced by solid-state fermentation by Rhizopus microsporus", Bioresour. Technol, 265, pp.52~58 (2018)
- Pitol LO, "Optimization studies to develop a low-cost medium for production of the lipases of Rhizopus microsporus by solid-state fermentation and scale-up of the process to a pilot packed-bed bioreactor", Process Biochem, 62, pp.37~47 (2017)
- Escaramboni B, "Ethanol biosynthesis by fast hydrolysis of cassava bagasse using fungal amylases produced in optimized conditions", Ind. Crop. Prod, 112, pp.368~377 (2018)
- Ramos-Sánchez LB, "Fungal lipase production by solid-state fermentation", J. Bioproc. Biotech, 5, pp.203~211 (2015)
- Diaz AB, "Modelling of different enzyme productions by solid-state fermentation on several agro-industrial residues", Appl. Microbiol. Biot, 100, pp.1~12 (2016)
- Casciatori FP, "Heat transfer in packedbeds of agricultural waste with low rates of air flow applicable to solid-state fermentation", Chem. Eng. Sci, 188, pp.97~111 (2018)
- Hansen GH, "Production of cellulolytic enzymes from ascomycetes:Comparison of solid state and submerged fermentation", Process Biochem, 50, pp.1327~1341 (2015)
- Kosakai Y, "Enhancement of L(+)-lactic acid production using mycelial flocs of Rhizopus oryzae", Biotechnol. Bioeng, 55, pp.461~470 (1997)
- Varzakas T., "Rhizopus oligosporus mycelial penetration and enzyme diffusion in soya bean tempe", Process Biochem, 33, pp.741~747 (1998)
- Rodríguez de Olmos A, "Optimization of fermentation parameters to study the behavior of selected lactic cultures on soy solid state fermentation", Int. J. Food Microbiol, 196, pp.16~23 (2015)
