양식 동자개(Pelteobagrus fulvidraco)의 Edwardsiella ictaluri 감염
Edwardsiella ictaluri Infection in Cultured Yellow Catfish Pelteobagrus fulvidraco Fingerlings in Korea
0
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
We observed yellow catfish Pelteobagrus fulvidraco fingerlings cultured in land ponds in Korea swimming in a corkscrew spiral pattern while hanging head-up and tail-down at the water surface, before eventually dying. Externally, these fish displayed “hole in the head” disease, pale gills, and hemorrhages in the base of the pectoral and caudal fins; internally they had liver hemorrhages and kidney discoloration. The bacterium Edwardsiella ictaluri (YCK-01 and YCL-01) was identified in the kidneys and livers of diseased fish via phenotypic characteristics and PCR analysis using the ictaluri-specific primers IVS (an intervening sequence) and IRS (the inter-ribosomal spacer). Infectivity challenges by intraperitoneal and immersion routes showed that a representative bacterial strain (YCK) exhibited strong virulence to yellow catfish, with an LD50 of 3.2 × 104 CFU/fish and 2.5 × 106 CFU/mL, respectively. This is the first report of E. ictaluri infection in yellow catfish from Korea.
Abstract
We observed yellow catfish Pelteobagrus fulvidraco fingerlings cultured in land ponds in Korea swimming in a corkscrew spiral pattern while hanging head-up and tail-down at the water surface, before eventually dying. Externally, these fish displayed “hole in the head” disease, pale gills, and hemorrhages in the base of the pectoral and caudal fins; internally they had liver hemorrhages and kidney discoloration. The bacterium Edwardsiella ictaluri (YCK-01 and YCL-01) was identified in the kidneys and livers of diseased fish via phenotypic characteristics and PCR analysis using the ictaluri-specific primers IVS (an intervening sequence) and IRS (the inter-ribosomal spacer). Infectivity challenges by intraperitoneal and immersion routes showed that a representative bacterial strain (YCK) exhibited strong virulence to yellow catfish, with an LD50 of 3.2 × 104 CFU/fish and 2.5 × 106 CFU/mL, respectively. This is the first report of E. ictaluri infection in yellow catfish from Korea.
참고문헌 (29)
- Baxa D, "Susceptibility of nonictalurid fishes to experimental infection withEdwardsiella ictaluri", Dis Aquat Org, 8, pp.113~117 (1990)
- Crumlish M, "Identification of Edwardsiella ictaluri from diseased freshwater catfish, Pangasiurs hypophthalus(Sauvage), cultured n the Mekong Delta, Vietnam", J Fish Dis, 25, pp.733~737 (2002)
- Geng Y, "Isolation, identification and biological characterization of Edwardsiella ictaluri from cultured yellow catfish Pelteobagrus fulvidraco", Oceanologia Et Limnologia Sinica, 41, pp.61~67 (2010)
- Hawke J, "A bacterium associated with disease of pond cultured channel catfish, Ictalurus punctatus", J Fish Res Bd Can, 36, pp.1508~1512 (1979)
- Hawke J, "Edwardsiella ictaluri sp. nov., the causative agent of enteric septicemia of catfish", Intl J Sys Bacteriol, 31, pp.396~400 (1981)
- Iwanowicz LR, "Mortality and pathology in brown bullheads Amieurus nebulosus associated with a spontaneous Edwardsiella ictaluri outbreak under tank culture conditions", Dis Aquat Org, 70, pp.219~225 (2006)
- Jarboe HH, "Pathology associated with a natural Edwardsiella ictaluri infection in channel catfish(Ictalurus punctatus Rafinesque)", J Wildlife Dis, 20, pp.352~354 (1984)
- Kasornchandra J, "Edwardsiella ictaluri from walking catfish, Clarias batrachus L., in Thailand", J Fish Dis, 10, pp.137~138 (1987)
- Keskun O, "Edwardsiella ictaluri infection in rainbow trout(Oncorynchus mykiss)", Turk J Vet Anim Sci, 28, pp.649~653 (2004)
- Klesius P, "Isolation of Edwardsiella ictaluri from tadpole madtom in a southwestern New Jersey River", J Aquat Anim Health, 15, pp.295~301 (2003)
- Liu JY, "Isolation and characterization of Edwardsiella ictaluri strains aspathogens from diseased yellow catfish Pelteobagrus fulvidraco(Richardson)cultured in China", Aquaculture Res, 41, pp.1835~1844 (2010)
- Morrison E, "Olfactory organ of channel catfish as a site of experimental Edwardsiella ictaluri infection", J Aquat Anim Health, 6, pp.101~109 (1994)
- Newton JC, "A. : Pathology of experimental enteric septicaemia in channel catfish, Ictalurus punctatus(Rafinesque), following immersion-exposure to Edwardsiella ictaluri", J Fish Dis, 12, pp.335~347 (1989)
- Pasnik DJ, "Experimental Edwardsiella ictaluri infection causes mortality in white perch(Morone americana)", J Anim Vet Adv, 6, pp.646~649 (2007)
- Park, "Studies on disease of catfish(Silurus asotus)in Korea. Ⅲ. Edwardsiella ictaluri infection", J Fish Pathol, 7, pp.105~112 (1994)
- Plumb J, "Susceptibility of European catfish(Silurus gianis)to Edwardsiella ictaluri", J Appl Microbiol, 3, pp.45~48 (1987)
- Plumb J, "Susceptibility of five species of fish to Edwardsiella ictaluri", J Fish Dis, 6, pp.261~266 (1983)
- Popovic NT, "Commercial phenotypic tests(API 20E)in diagnosis of fish bacteria : a review", Vet Med, 52, pp.49~53 (2007)
- Reed LJ, "A simple method of estimating 50 percent endpoints", Am J Hyg, 27, pp.493~497 (1938)
- Sakai T, "Outbreaks of Edwardsiella ictaluri infection in ayu Plecoglossu altivelis in Japanese rivers", Fish Pathol, 43, pp.152~157 (2008)
- Shotts E, "Pathogenesis of experimental Edwardsiella ictaluri infections in channel catfish(Ictalurus punctatus)", Can J Fish Aquat Sci, 43, pp.36~42 (1986)
- Soto E, "Edwardsiella ictaluri as the causative agent of mortality in cultured Nile tilapia", J Aquat Anim Health, 24, pp.81~89 (2012)
- Waltman W, "Recovery of Edwardsiella ictaluri from danio(Danio devario)", Aquaculture, 46, pp.63~66 (1985)
- Watman W, "Biochemical characteristics of Edwardsiella ictlarui", Appl Environ Microbiol, 51, pp.101~104 (1986)
- Williams ML, "Verification of an Edwardsiella ictaluri-specific diagnostic PCR", Lett Appl Microbiol, 50, pp.153~157 (2010)
- Yasuda K, "First isolation of Edwardsiella ictaluri from cultured striped catfish Pangasius hypophthalmus in Indonesia", Fish Pathol, 38, pp.181~183 (2003)
- Ye S, "First case of Edwardsiella ictaluri infection in China farmed yellow catfish Pelteobagrus fulvidraco", Aquaculture, 292, pp.6~10 (2009)
- Yi G, "Isolation and characterization of Edwardsiella ictaluri from cultured yellow catfish(Pelteobagrus fulvidraco)", Israeli J Aquaculture-Bamidgeh, 62, pp.105~115 (2010)
- Yu J-H, "Edwardsiella tarda infection in Korean catfish, Silurus asotus, in a Korean fish farm", Aquaculture Res, 41, pp.19~26 (2009)