Recovery of Pseudomonas anguilliseptica from Diseased Striped Beakperch (Oplegnathus fasciatus) in Korea
0
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
In recent years, several southern coastal fish farms in Korea have experienced 2-30% mortality in striped beakperch suffering from bacterial infections during the spring. In this study, we identified a bacterium isolated from diseased striped beakperch as Pseudomonas anguilliseptica via a biochemical test and 16S rDNA sequence analysis. To evaluate the susceptibility of striped beakperch to P. anguilliseptica, 4.39×107 or 4.39×105 CFU/fish of bacteria were injected intraperitoneally at 18±1°C into fish weighing 5.5 g. Cumulative mortalities reached 100% and 45% in the 4.39×107 and 4.39×105 CFU/fish infected groups, respectively. Experimentally infected fish showed cell-associated inflammation as well as bacteria in the kidneys and spleen. This study is the first report of striped beakperch mortality caused by P. anguilliseptica, which has pathogenicity in striped beakperch.
Abstract
In recent years, several southern coastal fish farms in Korea have experienced 2-30% mortality in striped beakperch suffering from bacterial infections during the spring. In this study, we identified a bacterium isolated from diseased striped beakperch as Pseudomonas anguilliseptica via a biochemical test and 16S rDNA sequence analysis. To evaluate the susceptibility of striped beakperch to P. anguilliseptica, 4.39×107 or 4.39×105 CFU/fish of bacteria were injected intraperitoneally at 18±1°C into fish weighing 5.5 g. Cumulative mortalities reached 100% and 45% in the 4.39×107 and 4.39×105 CFU/fish infected groups, respectively. Experimentally infected fish showed cell-associated inflammation as well as bacteria in the kidneys and spleen. This study is the first report of striped beakperch mortality caused by P. anguilliseptica, which has pathogenicity in striped beakperch.
참고문헌 (19)
- Berthe FCJ, "Identifi-cation of Pseudomonas anguilliseptica isolated from several fish species in France", Dis Aquat Org, 21, pp.151~155 (1995)
- Chao CB, "Histological, ultrastructural, and in situ hybridization study on enlarged cells in grouper Epinephelus hybrids infected by grouper iridovirus in Taiwan (TGIV)", Dis Aquat Org, 58, pp.127~142 (2004)
- Doménech A, "Association of Pseudomonas anguilliseptica infection with 'winter disease' in sea bream, Sparus aurata L", J Fish Dis, 22, pp.69~71 (1999)
- Doménech A, "Association of Pseudomonas anguilliseptica infection with 'winter disease' in sea bream, Sparus aurata L", Aquaculture, 156, pp.317~326 (1997)
- Ferguson HW, "Pseudomonas anguilliseptica infection in farmed cod, Gadus morhua L", J Fish Dis, 27, pp.249~253 (2004)
- Haenen OL, "First isolation and pathogenicity studies with Pseudomonas anguilli-septica from diseased European eel Anguilla anguilla (L.) in The Netherlands", Aquaculture, 196, pp.27~36 (2001)
- Jung SJ, "Iridovirus-like infection associated with high mortalities of striped beakperch, Oplegnathus fasciatus (Temminck et Schlegel), in southern coastal areas of the Korean peninsula", J Fish Dis, 23, pp.223~226 (2000)
- Kimura M, "A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences", J Mol Evol, 16, pp.111~120 (1980)
- Kusuda R, "Pseudomonas anguilliseptica infection of striped jack", Fish Pathol, 30, pp.121~122 (1995)
- Muroga K, "Studies on red spot disease of pond-cultured eels-I. The occurrence of the disease in eel culture ponds in Tokushima prefecture in 1972", Fish Pathol, 8, pp.1~9 (1972)
- Nakai T, "The fate of Pseudomonas anguilliseptica in arti-ficially infected eels Anguilla japonica", Fish Pathol, 19, pp.253~258 (1985)
- Nakajima K, "Comparison of fatty acid, protein and serological properties distinguishing outer membranes of Pseudomonas anguilliseptica strains from those of fish pathogens and other Pseudomonads", Int J Systemat Bacteriol, 33, pp.1~8 (1983)
- Oh MJ, "Susceptibility of marine fish species to a megalocytivirus, turbot iridovirus, isolated from turbot, Psetta maximus (L.)", J Fish Dis, 29, pp.415~421 (2006)
- 오승용, "돌돔, Oplegnathus fasciatus 치어의 절식시 산소 소비율에 미치는 수온과 광주기의 영향", Ocean and Polar Research, 28(4), pp.407~413 (2006), 한국해양연구원
- Saitou N, "The neighbor-joining method: a new method for reconstructing phylogenetic trees", Mol Biol Evol, 4, pp.406~425 (1987)
- Tort L, "Serum hemolytic and agglutinating activity as indicators of fish immunocompetence: their suitability in stress and dietary studies", Aqua Int, 4, pp.31~41 (1996)
- Wakabayashi H, "Characteristics of a Pseudomonas sp. from an epizootic of pond-cultured eels (Anguilla japonica)", Bull Jap Soc Sci Fish, 38, pp.577~587 (1972)
- Weisburg WG, "16S ribosomal DNA amplification for phylogenetic study", J Bacteriol, 173, pp.697~703 (1991)
- Wiklund T, "Pseudomonas anguilliseptica as a pathogen of salmonid fish in Finland", Dis Aquat Org, 8, pp.13~19 (1990)