Receptor activator of nuclear factor kappa-B gene polymorphisms in Iranian periodontitis and peri-implantitis patients
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Purpose: Peri-implantitis and periodontitis are inflammatory and infectious diseases of implantand tooth-supporting tissues. Recently, the role of gene polymorphisms of immune responsecomponents in the relevant pathogenesis has been investigated. The present study wasthe first to evaluate the relationship between two known single nucleotide polymorphisms(SNPs) of the receptor activator of nuclear factor kappa-β (RANK) gene (rs3018362 andrs35211496) in chronic periodontitis and peri-implantitis patients in an Iranian population. Methods: Eighty-one periodontally healthy patients, 38 patients with peri-implantitis, and74 patients with chronic periodontitis were enrolled in this study. DNA was extracted fromblood arm vein samples by using Miller’s salting out technique according to the manufacturer’sinstructions given in the extraction kit. The concentration of DNA samples was measuredusing a spectrophotometer. The genetic polymorphisms of the RANK gene were evaluatedusing a competitive allele specific polymerase chain reaction (KBioscience allele specificPCR) technique. Differences in the frequencies of genotypes and alleles in the diseasedand healthy groups were analyzed using chi-squared statistical tests (P<0.05). Results: Analysis of rs35211496 revealed statistically significant differences in the expressionof the TT, TC, and CC genotypes among the three groups (P=0.00). No statistically significantdifference was detected in this respect between the control group and the chronicperiodontitis group. The expression of the GG, GA, and AA genotypes and allele frequencies(rs3018362) showed no statistically significant difference among the three groups (P=0.21). Conclusions: The results of this study indicate that the CC genotype of the rs35211496RANK gene polymorphism was significantly associated with peri-implantitis and may beconsidered a genetic determinant for peri-implantitis, but this needs to be confirmed byfurther studies in other populations.
Abstract
Purpose: Peri-implantitis and periodontitis are inflammatory and infectious diseases of implantand tooth-supporting tissues. Recently, the role of gene polymorphisms of immune responsecomponents in the relevant pathogenesis has been investigated. The present study wasthe first to evaluate the relationship between two known single nucleotide polymorphisms(SNPs) of the receptor activator of nuclear factor kappa-β (RANK) gene (rs3018362 andrs35211496) in chronic periodontitis and peri-implantitis patients in an Iranian population. Methods: Eighty-one periodontally healthy patients, 38 patients with peri-implantitis, and74 patients with chronic periodontitis were enrolled in this study. DNA was extracted fromblood arm vein samples by using Miller’s salting out technique according to the manufacturer’sinstructions given in the extraction kit. The concentration of DNA samples was measuredusing a spectrophotometer. The genetic polymorphisms of the RANK gene were evaluatedusing a competitive allele specific polymerase chain reaction (KBioscience allele specificPCR) technique. Differences in the frequencies of genotypes and alleles in the diseasedand healthy groups were analyzed using chi-squared statistical tests (P<0.05). Results: Analysis of rs35211496 revealed statistically significant differences in the expressionof the TT, TC, and CC genotypes among the three groups (P=0.00). No statistically significantdifference was detected in this respect between the control group and the chronicperiodontitis group. The expression of the GG, GA, and AA genotypes and allele frequencies(rs3018362) showed no statistically significant difference among the three groups (P=0.21). Conclusions: The results of this study indicate that the CC genotype of the rs35211496RANK gene polymorphism was significantly associated with peri-implantitis and may beconsidered a genetic determinant for peri-implantitis, but this needs to be confirmed byfurther studies in other populations.
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