Effects of type 2 diabetes mellitus on expression of macrophage colony-stimulating factor and bone differentiation factors in human chronic periodontitis
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Purpose: The purpose of this study was to compare and quantify the expression of macrophage colony-stimulationg factor (M-CSF), receptor activator of nuclear factor kappa-B (RANK) and osteoprotegerin (OPG) that the related to bone resorption in the gingival tissues of patients with type 2 diabetes mellitus (DM) and healthy adults with chronic periodontitis. Materials and Methods: Depending on the patient's systemic condition and clinical criteria of gingiva, each gingival sample was divided into three groups. Group 1 (n=12) is clinically healthy gingiva obtained from systemically healthy 12 patients. Group 2 (n=12) is infl amed gingiva from patients with chronic periodontitis. Group 3 (n=12) is infl amed gingiva from patients with chronic periodontitis associated with type 2 DM. Tissue samples were prepared and analyzed by Western blotting. The relative quantifi cations of M-CSF, RANK and OPG were performed with a densitometer. Results: The expression levels of M-CSF signifi cantly increased in group 3 as compared to group 1 (p<0.05). The expression levels of RANK were signifi cantly increased in group 3 as compared to group 1 and 2. And In the group 2, the levels of RANK were signifi cantly increased as compared to group 1 (p<0.05). The expression levels of OPG were signifi cantly increased in the group 3 as compared to group 1 and 2 (p<0.05). Conclusion: In conclusion, It can be assumed that M-CSF, RANK and OPG may be involved in the progression of periodontal inflammation associated to type 2 DM. This study demonstrated that the expression levels of RANK and OPG might be infl ammatory markers in periodontal infl amed tissue.
Abstract
Purpose: The purpose of this study was to compare and quantify the expression of macrophage colony-stimulationg factor (M-CSF), receptor activator of nuclear factor kappa-B (RANK) and osteoprotegerin (OPG) that the related to bone resorption in the gingival tissues of patients with type 2 diabetes mellitus (DM) and healthy adults with chronic periodontitis. Materials and Methods: Depending on the patient's systemic condition and clinical criteria of gingiva, each gingival sample was divided into three groups. Group 1 (n=12) is clinically healthy gingiva obtained from systemically healthy 12 patients. Group 2 (n=12) is infl amed gingiva from patients with chronic periodontitis. Group 3 (n=12) is infl amed gingiva from patients with chronic periodontitis associated with type 2 DM. Tissue samples were prepared and analyzed by Western blotting. The relative quantifi cations of M-CSF, RANK and OPG were performed with a densitometer. Results: The expression levels of M-CSF signifi cantly increased in group 3 as compared to group 1 (p<0.05). The expression levels of RANK were signifi cantly increased in group 3 as compared to group 1 and 2. And In the group 2, the levels of RANK were signifi cantly increased as compared to group 1 (p<0.05). The expression levels of OPG were signifi cantly increased in the group 3 as compared to group 1 and 2 (p<0.05). Conclusion: In conclusion, It can be assumed that M-CSF, RANK and OPG may be involved in the progression of periodontal inflammation associated to type 2 DM. This study demonstrated that the expression levels of RANK and OPG might be infl ammatory markers in periodontal infl amed tissue.
참고문헌 (40)
- 백영란, "만성 치주염 환자의 치은 조직에서 RANK 및 RANKL의 발현", Journal of Periodontal & Implant Science, 37(4), pp.849~857 (2007), 대한치주과학회
- Bickel M, "Cytokine gene expression in chronic periodontitis", J Clin Periodontol, 28, pp.840~847 (2001)
- 채영미, "Upregulation of smpd3 via BMP2 stimulation and Runx2", BMB reports, 42(2), pp.86~90 (2009), 한국생화학분자생물학회
- Cho JY, "Expression and activity of human Na+", Isymporter in human glioma cells by adenovirus-mediated gene delivery, 2000, pp.7, 740~749
- Costa PP, "Salivary interleukin-6, matrix metalloproteinase-8, and osteoprotegerin in patients with periodontitis and diabetes", J Periodontol, 81, pp.384~391 (2010)
- Crotti T, "Receptor activator NF kappaB ligand (RANKL) and osteoprotegerin (OPG) protein expression in periodontitis", J Periodontal Res, 38, pp.380~387 (2003)
- Emrich LJ, "Periodontal disease in noninsulin- dependent diabetes mellitus", J Periodontol, 62, pp.123~131 (1991)
- Fixe P, "M-CSF: haematopoietic growth factor or infl ammatory cytokine?", Cytokine, 10, pp.32~37 (1998)
- Grossi SG, "Assessment of risk for periodontal disease. I. Risk indicators for attachment loss", J Periodontol, 65, pp.260~267 (1994)
- Hormdee D, "Protein kinase-A-dependent osteoprotegerin production on interleukin-1 stimulation in human gingival fibroblasts is distinct from periodontal ligament fibroblasts", Clin Exp Immunol, 142, pp.490~497 (2005)
- Hugoson A, "Periodontal conditions in insulin-dependent diabetics", J Clin Periodontol, 16, pp.215~223 (1989)
- Iacopino AM, "Periodontitis and diabetes interrelationships: role of infl ammation", Ann Periodontol, 6, pp.125~137 (2001)
- 주상돈, "단순 만성 치주염 환자 및 2형 당뇨병환자의 만성 치주염 치은조직에서 염증성 매개인자의 발현 양상 비교", Journal of Periodontal & Implant Science, 37(5), pp.353~369 (2007), 대한치주과학회
- Katagiri T, "Regulatory mechanisms of osteoblast and osteoclast diff erentiation", Oral Dis, 8, pp.147~159 (2002)
- Kawano Y, "The effect of inflammatory cytokines on secretion of macrophage colony-stimulating factor and monocyte chemoattractant protein-1 in human granulosa cells", Am J Reprod Immunol, 52, pp.124~128 (2004)
- Lacey DL, "Osteoprotegerin ligand is a cytokine that regulates osteoclast differentiation and activation", Cell, 93, pp.165~176 (1998)
- Liu D, "Expression of RANKL and OPG mRNA in periodontal disease: possible involvement in bone destruction", Int J Mol Med, 11, pp.17~21 (2003)
- McCauley LK, "Mediators of periodontal osseous destruction and remodeling: principles and implications for diagnosis and therapy", J Periodontol, 73, pp.1377~1391 (2002)
- Mizuno N, "Characterization of epithelial cells derived from periodontal ligament by gene expression patterns of bonerelated and enamel proteins", Cell Biol Int, 29, pp.111~117 (2005)
- Mühlemann HR, "Gingival sulcus bleeding--a leading symptom in initial gingivitis", Helv Odontol Acta, 15, pp.107~113 (1971)
- Nabipour I, "Osteoprotegerin in relation to type 2 diabetes mellitus and the metabolic syndrome in postmenopausal women", Metabolism, 59, pp.742~747 (2010)
- Nagasawa T, "Roles of receptor activator of nuclear factor-kappaB ligand (RANKL) and osteoprotegerin in periodontal health and disease", Periodontol 2000, 43, pp.65~84 (2007)
- Page RC, "The role of infl ammatory mediators in the pathogenesis of periodontal disease", J Periodontal Res, 26(3), pp.230~242 (1991)
- 박재완, "단순 만성 치주염 환자 및 2형 당뇨병환자의 만성 치주염 치은조직에서 IL-6, elastase 및 α1-PI의 발현 양상 비교", Journal of Periodontal & Implant Science, 37(5), pp.325~338 (2007), 대한치주과학회
- Pontes Andersen CC, "Relationship between periodontitis and diabetes: lessons from rodent studies", J Periodontol, 78, pp.1264~1275 (2007)
- Rani CS, "Dental cells express factors that regulate bone resorption", Mol Cell Biol Res Commun, 3, pp.145~152 (2000)
- Sakata M, "Expression of osteoprotegerin (osteoclastogenesis inhibitory factor) in cultures of human dental mesenchymal cells and epithelial cells", J Bone Miner Res, 14, pp.1486~1492 (1999)
- Salvi GE, "Inflammatory mediator response as a potential risk marker for periodontal diseases in insulin-dependent diabetes mellitus patients", J Periodontol, 68, pp.127~135 (1997)
- Simonet WS, "Osteoprotegerin: a novel secreted protein involved in the regulation of bone density", Cell, 89, pp.309~319 (1997)
- Suda T, "Modulation of osteoclast differentiation", Endocr Rev, 13, pp.66~80 (1992)
- Suda T, "Modulation of osteoclast differentiation and function by the new members of the tumor necrosis factor receptor and ligand families", Endocr Rev, 20, pp.345~357 (1999)
- Tanaka S, "Macrophage colonystimulating factor is indispensable for both proliferation and differentiation of osteoclast progenitors", J Clin Invest, 91, pp.257~263 (1993)
- Teng YT, "Functional human T-cell immunity and osteoprotegerin ligand control alveolar bone destruction in periodontal infection", J Clin Invest, 106, pp.R59~R67 (2000)
- Theill LE, "RANK-L and RANK: T cells, bone loss, and mammalian evolution", Annu Rev Immunol, 20, pp.795~823 (2002)
- Wada T, "RANKL-RANK signaling in osteoclastogenesis and bone disease", Trends Mol Med, 12, pp.17~25 (2006)
- Yalda B, "Diabetes as a modifier of periodontal disease expression", Periodontol 2000, 6, pp.37~49 (1994)
- Yamazaki H, "Presence of osteoclast precursors in colonies cloned in the presence of hematopoietic colony-stimulating factors", Exp Hematol, 29, pp.68~76 (2001)
- Yasuda H, "Osteoclast differentiation factor is a ligand for osteoprotegerin", osteoclastogenesis-inhibitory factor and is identical to TRANCE, Proc Natl Acad Sci U S A, pp.1998, 95[DOI]
- Yasuda H, "Identity of osteoclastogenesis inhibitory factor (OCIF) and osteoprotegerin (OPG): a mechanism by which OPG", OCIF inhibits osteoclastogenesis in vitro, 1998, pp.139, 1329~1337
- Yaturu S, "Relationship of elevated osteoprotegerin with insulin resistance, CRP, and TNF-alpha levels in men with type 2 diabetes", Cytokine, 44, pp.168~171 (2008)
