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Effects of type 2 diabetes mellitus on expression of macrophage colony-stimulating factor and bone differentiation factors in human chronic periodontitis

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Purpose: The purpose of this study was to compare and quantify the expression of macrophage colony-stimulationg factor (M-CSF), receptor activator of nuclear factor kappa-B (RANK) and osteoprotegerin (OPG) that the related to bone resorption in the gingival tissues of patients with type 2 diabetes mellitus (DM) and healthy adults with chronic periodontitis. Materials and Methods: Depending on the patient's systemic condition and clinical criteria of gingiva, each gingival sample was divided into three groups. Group 1 (n=12) is clinically healthy gingiva obtained from systemically healthy 12 patients. Group 2 (n=12) is infl amed gingiva from patients with chronic periodontitis. Group 3 (n=12) is infl amed gingiva from patients with chronic periodontitis associated with type 2 DM. Tissue samples were prepared and analyzed by Western blotting. The relative quantifi cations of M-CSF, RANK and OPG were performed with a densitometer. Results: The expression levels of M-CSF signifi cantly increased in group 3 as compared to group 1 (p<0.05). The expression levels of RANK were signifi cantly increased in group 3 as compared to group 1 and 2. And In the group 2, the levels of RANK were signifi cantly increased as compared to group 1 (p<0.05). The expression levels of OPG were signifi cantly increased in the group 3 as compared to group 1 and 2 (p<0.05). Conclusion: In conclusion, It can be assumed that M-CSF, RANK and OPG may be involved in the progression of periodontal inflammation associated to type 2 DM. This study demonstrated that the expression levels of RANK and OPG might be infl ammatory markers in periodontal infl amed tissue.

Abstract

Purpose: The purpose of this study was to compare and quantify the expression of macrophage colony-stimulationg factor (M-CSF), receptor activator of nuclear factor kappa-B (RANK) and osteoprotegerin (OPG) that the related to bone resorption in the gingival tissues of patients with type 2 diabetes mellitus (DM) and healthy adults with chronic periodontitis. Materials and Methods: Depending on the patient's systemic condition and clinical criteria of gingiva, each gingival sample was divided into three groups. Group 1 (n=12) is clinically healthy gingiva obtained from systemically healthy 12 patients. Group 2 (n=12) is infl amed gingiva from patients with chronic periodontitis. Group 3 (n=12) is infl amed gingiva from patients with chronic periodontitis associated with type 2 DM. Tissue samples were prepared and analyzed by Western blotting. The relative quantifi cations of M-CSF, RANK and OPG were performed with a densitometer. Results: The expression levels of M-CSF signifi cantly increased in group 3 as compared to group 1 (p<0.05). The expression levels of RANK were signifi cantly increased in group 3 as compared to group 1 and 2. And In the group 2, the levels of RANK were signifi cantly increased as compared to group 1 (p<0.05). The expression levels of OPG were signifi cantly increased in the group 3 as compared to group 1 and 2 (p<0.05). Conclusion: In conclusion, It can be assumed that M-CSF, RANK and OPG may be involved in the progression of periodontal inflammation associated to type 2 DM. This study demonstrated that the expression levels of RANK and OPG might be infl ammatory markers in periodontal infl amed tissue.

참고문헌 (40)

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