Effect of Long Term Reverse Feeding on the Reproductive and Non-reproductive Tissues in Male Mice
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Previously, we demonstrated that the shift and/or restriction of feeding time during relatively short-termperiod (4 weeks) could alter the pituitary gonadotropin expression and the weights of seminal vesicle and prostate in rats. Wealso found that the reverse feeding (RF) schedule (up to 8 weeks) might induce an adaptable metabolic stress and causeimpairment of androgen-dependent reproductive tissues. In the present study, we extended the RF time regimen up to 12weeks, and measured the reproductive tissue weights. After 4 and 8 weeks of RF, the weights of epididymis were notsignificantly different. After 12 weeks, however, epididymis weights of RF animals were significantly different (CON 12W :RF 12W = 48.26±0.62 mg : 44.05±1.57 mg, p<0.05). After 4 and 12 weeks of feeding, seminal vesicle weights of RF animalswere significantly decreased (CON 4W : RF 4W = 79.36±8.34 mg : 46.28±2.43 mg, p<0.001; CON 12W : RF 12W =72.04±3.76 mg : 46.71±2.27 mg, p<0.001, respectively). Prostate weights were not changed by RF. Kidney and spleenweights of RF animals were significantly different on weeks 4 and 12 (Kidney, CON 4W : RF 4W = 249.72±4.20 mg :228.41±3.03 mg, p<0.001; CON 12W : RF 12W = 309.15±7.49 mg : 250.72±6.13 mg, p<0.001, respectively, Spleen, CON4W : RF 4W = 111.26±3.76 mg : 96.88±4.69 mg, p<0.05; CON 12W : RF 12W = 123.93±10.72 mg : 94.68±5.65 mg, p<0.05,respectively). Histology analysis of seminal vesicle revealed that the thinner epithelial cell layers, reduced complexities ofswollen papilla folding in the exocrine glands on weeks 4 and 12 of RF. There was no histological difference between controland RF group on week 8. The present study indicates that up to 12 weeks RF induced differential changes in tissue weights ofmale mice. In particular, seminal vesicle, kidney and spleen seemed to temporarily adapted to the RF-induced metabolic stresson week 8 of feeding schedule. These results confirmed the our previous study that the RF might induce an adaptablemetabolic stress and cause impairment of androgen-dependent reproductive tissues such as epididymis and seminal vesicle aswell as non-reproductive tissues such as kidney and spleen. Further studies will be needed to achieve a better understanding ofthe how does mealtime shift affect the reproductive function and exact nature of adaptation.
Abstract
Previously, we demonstrated that the shift and/or restriction of feeding time during relatively short-termperiod (4 weeks) could alter the pituitary gonadotropin expression and the weights of seminal vesicle and prostate in rats. Wealso found that the reverse feeding (RF) schedule (up to 8 weeks) might induce an adaptable metabolic stress and causeimpairment of androgen-dependent reproductive tissues. In the present study, we extended the RF time regimen up to 12weeks, and measured the reproductive tissue weights. After 4 and 8 weeks of RF, the weights of epididymis were notsignificantly different. After 12 weeks, however, epididymis weights of RF animals were significantly different (CON 12W :RF 12W = 48.26±0.62 mg : 44.05±1.57 mg, p<0.05). After 4 and 12 weeks of feeding, seminal vesicle weights of RF animalswere significantly decreased (CON 4W : RF 4W = 79.36±8.34 mg : 46.28±2.43 mg, p<0.001; CON 12W : RF 12W =72.04±3.76 mg : 46.71±2.27 mg, p<0.001, respectively). Prostate weights were not changed by RF. Kidney and spleenweights of RF animals were significantly different on weeks 4 and 12 (Kidney, CON 4W : RF 4W = 249.72±4.20 mg :228.41±3.03 mg, p<0.001; CON 12W : RF 12W = 309.15±7.49 mg : 250.72±6.13 mg, p<0.001, respectively, Spleen, CON4W : RF 4W = 111.26±3.76 mg : 96.88±4.69 mg, p<0.05; CON 12W : RF 12W = 123.93±10.72 mg : 94.68±5.65 mg, p<0.05,respectively). Histology analysis of seminal vesicle revealed that the thinner epithelial cell layers, reduced complexities ofswollen papilla folding in the exocrine glands on weeks 4 and 12 of RF. There was no histological difference between controland RF group on week 8. The present study indicates that up to 12 weeks RF induced differential changes in tissue weights ofmale mice. In particular, seminal vesicle, kidney and spleen seemed to temporarily adapted to the RF-induced metabolic stresson week 8 of feeding schedule. These results confirmed the our previous study that the RF might induce an adaptablemetabolic stress and cause impairment of androgen-dependent reproductive tissues such as epididymis and seminal vesicle aswell as non-reproductive tissues such as kidney and spleen. Further studies will be needed to achieve a better understanding ofthe how does mealtime shift affect the reproductive function and exact nature of adaptation.
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