Effect of Feeding Time Shift on the Reproductive System in Male Rats
2
조회
0
다운로드
2
인용
0
참고문헌
키워드
초록
Circadian rhythmicity (e.g. hormone secretory pattern of hormones) plays an important role in the control of reproductive function. We hypothesized that the alteration of feeding pattern via meal time shift/restriction might disrupt circadian rhythms in energy balance and induce changes in reproductive activities. To test this hypothesis, we employed simple animal model that not allowing ad libitum feeding but daytime only feeding. The animals of ad libitum feeding group (Control) have free access to food for 4 weeks. The day feeding (=reverse feeding) animals (RF group) have restricted access to food during daytime (09:00-1800) for 4 weeks. After 4 weeks of feeding, body weights, testis and epididymis weights of animals from both group were not significantly different However, the weights of seminal vesicle (control:RF group=0.233±0.014 g:0.188±0.009 g, p<0.01) and prostate (control:RF group=0.358±0.015 g:0.259±0.015 g, p<0.001) were significantly lower in RF group animals. The mRNA levels of pituitary common alpha subunit (Cα; control : RF group=1.0±0.0699 AU : 0.1923±0.0270AU, p<0.001, Fig. 1A) and FSHβ (control : RF group= 1.0±0.1489AU :0.5237±0.1088AU, p<0.05, Fig. 1C) were significantly decreased in RF group. The mRNA levels of ACTH were not significantly different. We were unable to find any prominent difference in the microstructures of epididymis and slight alterations in the those of seminal vesicles after 4 weeks of reversed feeding when compared to control samples. The present study demonstrates that the shift and/or restriction of feeding time could alter the pituitary gonadotropin expression and the weights of seminal vesicle and prostate in rats. These data suggest the lowered gonadotropin inputs may decreases androgen secretion form testis, and consequently results in poor response of androgen-dependent tissues such as seminal vesicle and prostate.
Abstract
Circadian rhythmicity (e.g. hormone secretory pattern of hormones) plays an important role in the control of reproductive function. We hypothesized that the alteration of feeding pattern via meal time shift/restriction might disrupt circadian rhythms in energy balance and induce changes in reproductive activities. To test this hypothesis, we employed simple animal model that not allowing ad libitum feeding but daytime only feeding. The animals of ad libitum feeding group (Control) have free access to food for 4 weeks. The day feeding (=reverse feeding) animals (RF group) have restricted access to food during daytime (09:00-1800) for 4 weeks. After 4 weeks of feeding, body weights, testis and epididymis weights of animals from both group were not significantly different However, the weights of seminal vesicle (control:RF group=0.233±0.014 g:0.188±0.009 g, p<0.01) and prostate (control:RF group=0.358±0.015 g:0.259±0.015 g, p<0.001) were significantly lower in RF group animals. The mRNA levels of pituitary common alpha subunit (Cα; control : RF group=1.0±0.0699 AU : 0.1923±0.0270AU, p<0.001, Fig. 1A) and FSHβ (control : RF group= 1.0±0.1489AU :0.5237±0.1088AU, p<0.05, Fig. 1C) were significantly decreased in RF group. The mRNA levels of ACTH were not significantly different. We were unable to find any prominent difference in the microstructures of epididymis and slight alterations in the those of seminal vesicles after 4 weeks of reversed feeding when compared to control samples. The present study demonstrates that the shift and/or restriction of feeding time could alter the pituitary gonadotropin expression and the weights of seminal vesicle and prostate in rats. These data suggest the lowered gonadotropin inputs may decreases androgen secretion form testis, and consequently results in poor response of androgen-dependent tissues such as seminal vesicle and prostate.
참고문헌 (24)
- Angeles-Castellanos M, "The suprachiasmatic nucleus participates in food entrainment: a lesion study", Neuroscience, 165, pp.1115~1126 (2010)
- Boden MJ, "Circadian rhythms and reproduction", Reproduction, 132, pp.379~392 (2006)
- Castellano JM, "Kisspeptins: bridging energy homeostasis and reproduction", Brain Res, 1364, pp.129~138 (2010)
- Castellano JM, "KiSS-1", kisspeptins and the metabolic control of reproduction: physiologic roles and putative physiopathological implications, 2009, pp.30, 139~145
- Damiola F, "Restricted feeding uncouples circadian oscillators in peripheral tissues from the central pacemaker in the suprachiasmatic nucleus", Genes Dev, 14, pp.2950~2961 (2000)
- Assis MA, "Food intake and circadian rhythms in shift workers with a high workload", Appetite, 40, pp.175~183 (2003)
- Escobar C, "Persistence of metabolic rhythmicity during fasting and its entrainment by restricted feeding schedules in rats", Am J Physiol Regul Integr Comp Physiol, 274, pp.1309~1316 (1998)
- Glad CA, "Reverse feeding suppresses the activity of the GH axis in rats and induces a preobesogenic state", Endocrinology, 152, pp.869~882 (2011)
- Gooley JJ, "The dorsomedial hypothalamic nucleus is critical for the expression of food-entrainable circadian rhythms", Nat Neurosci, 9, pp.398~407 (2006)
- Hill JW, "Hypothalamic pathways linking energy balance and reproduction", Am J Physiol Endocrinol Metab, 294, pp.827~832 (2008)
- Eun Young Jeon, "Effect of High-Fat Diet Feeding on the Reproductive System in Male Rats", 발생과 생식, 15(3), pp.265~272 (2011), 한국발생생물학회
- Karlsson B, "Is there an association between shift work and having a metabolic syndrome? Results from a population based study of 27,485 people", Occup Environ Med, 58, pp.747~752 (2001)
- Knutsson A, "Health disorders of shift workers", Occup Med, 53, pp.103~108 (2003)
- Krieger DT, "Suprachiasmatic nuclear lesions do not abolish food-shifted circadian adrenal and temperature rhythmicity", Science, 197, pp.398~399 (1977)
- Lennernas M, "Shift related dietary intake in day and shift workers", Appetite, 25, pp.253~265 (1995)
- Martínez-Merlos MT, "Dissociation between adipose tissue signals, behaviour and the food-entrained oscillator", J Endocrinol, 181, pp.53~63 (2004)
- Milano W, "Night eating syndrome: an overview", J Pharm Pharmacol, 64, pp.2~10 (2012)
- Mistlberger RE, "Neurobiology of food anticipatory circadian rhythms", Physiol Behav, 26(104), pp.535~545 (2011)
- Pasqua IC, "The nutritional status and eating habits of shift workers: a chronobiological approach", Chronobiol Int, 21, pp.949~960 (2004)
- Salgado-Delgado R, "Food intake during the normal activity phase prevents obesity and circadian desynchrony in a rat model of night work", Endocrinology, 151, pp.1019~1029 (2010)
- Schibler U, "Peripheral circadian oscillators in mammals: time and food", J Biol Rhythms, 18, pp.250~260 (2003)
- Stephan FK, "The "other" circadian system: food as a Zeitgeber", J Biol Rhythms, 17, pp.284~292 (2002)
- Urbanski HF, "Role of circadian neuroendocrine rhythms in the control of behavior and physiology", Neuroendocrinology, 93, pp.211~222 (2011)
- Vener K, "The effect of shift work on gastrointestinal function: a review", Chronobiologia, 16, pp.421~439 (1989)