Effect of Reverse Feeding on the Reproductive System in Male Rats
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초록
Circadian timing system plays a major role in a wide range of reproductive function. However it is plausible idea that other environmental and/or internal cue might be simultaneously participated in the optimal regulation of reproductive system. In the present study we extended the reverse feeding (RF) time regimen up to 8 weeks, then measured the general and reproductive indices of the animals. The animals of ad libitum feeding group (Control, CON) have free access to food for 4, 6 and 8 weeks, respectively. The day feeding animals (reverse feeding, RF group) have restricted access to food during daytime (09:00-18:00) for 4, 6 and 8 weeks, respectively. When the feeding schedules were over,key indices were measured. After 4 weeks and 8 weeks of feeding, body weights of animals were not significantly different. However, body weights of 6 weeks RF animals were significantly smaller than those of control animals (CON : RF =333.46±12.71 g : 289.91±8.31 g, p<0.01). The blood glucose levels of 4 weeks RF animals were significantly decreased compared to the levels of control animals (CON : RF = 161.4±2.7 mg/dL : 176.7±5 mg/dL, p<0.01) while the levels of 6 weeks RF and 8 weeks RF animals were not different form those of control animals. Reproductive and non-reproductive tissue weights from 6 weeks RF group were significantly lowered than those from CON group (testis, CON : RF =1.4714±0.0174 g : 1.3724±0.0168 g, p<0.001; epididymis, CON : RF = 0.3574±0.0059 g : 0.3243±0.0068 g, p<0.001;seminal vesicle, CON : RF = 0.1655±0.0068 g : 0.1328±0.0054 g, p<0.001; prostate, CON : RF = 0.3350±0.0231 g :0.2528±0.0143 g, p<0.01). After 4 weeks and 8 weeks of reverse feeding, sperm counts in RF animals were markedly reduced than those in control animals[CON 4W : RF 4W = 121.17±9.96 (×106) : 50.86±9 (×106), p<0.001; CON 8W :RF 8W= 138.69±9.8 (×106) : 108.94±4.22 (×106), p<0.001]. Present study indicates that RF may induce an adaptable metabolic stress and cause impairment of androgen-dependent reproductive tissues. On-going longitudinal studies will allow a better understanding of the how does mealtime shift affect the reproductive function and exact nature of adaptation.
Abstract
Circadian timing system plays a major role in a wide range of reproductive function. However it is plausible idea that other environmental and/or internal cue might be simultaneously participated in the optimal regulation of reproductive system. In the present study we extended the reverse feeding (RF) time regimen up to 8 weeks, then measured the general and reproductive indices of the animals. The animals of ad libitum feeding group (Control, CON) have free access to food for 4, 6 and 8 weeks, respectively. The day feeding animals (reverse feeding, RF group) have restricted access to food during daytime (09:00-18:00) for 4, 6 and 8 weeks, respectively. When the feeding schedules were over,key indices were measured. After 4 weeks and 8 weeks of feeding, body weights of animals were not significantly different. However, body weights of 6 weeks RF animals were significantly smaller than those of control animals (CON : RF =333.46±12.71 g : 289.91±8.31 g, p<0.01). The blood glucose levels of 4 weeks RF animals were significantly decreased compared to the levels of control animals (CON : RF = 161.4±2.7 mg/dL : 176.7±5 mg/dL, p<0.01) while the levels of 6 weeks RF and 8 weeks RF animals were not different form those of control animals. Reproductive and non-reproductive tissue weights from 6 weeks RF group were significantly lowered than those from CON group (testis, CON : RF =1.4714±0.0174 g : 1.3724±0.0168 g, p<0.001; epididymis, CON : RF = 0.3574±0.0059 g : 0.3243±0.0068 g, p<0.001;seminal vesicle, CON : RF = 0.1655±0.0068 g : 0.1328±0.0054 g, p<0.001; prostate, CON : RF = 0.3350±0.0231 g :0.2528±0.0143 g, p<0.01). After 4 weeks and 8 weeks of reverse feeding, sperm counts in RF animals were markedly reduced than those in control animals[CON 4W : RF 4W = 121.17±9.96 (×106) : 50.86±9 (×106), p<0.001; CON 8W :RF 8W= 138.69±9.8 (×106) : 108.94±4.22 (×106), p<0.001]. Present study indicates that RF may induce an adaptable metabolic stress and cause impairment of androgen-dependent reproductive tissues. On-going longitudinal studies will allow a better understanding of the how does mealtime shift affect the reproductive function and exact nature of adaptation.
참고문헌 (27)
- Antunes LC, "Hidalgo MP Obesity and shift work: Chronobiological aspects", Nutr Res Rev, 23, pp.155~168
- Boden MJ, "Circadian rhythms and reproduction", Reproduction, 132, pp.379~392 (2006)
- Bray GA, "Obesity and reproduction", Hum Reprod, 12(1), pp.26~32 (1997)
- Castellano JM, "KiSS-1", kisspeptins and the metabolic control of reproduction: Physiologic roles and putative physiopathological implications, 2009, pp.30, 139~145
- Clermont Y, "Duration of the cycle of the seminiferous epithelium of normal, hypophysectomizedhormone treated albino rats", Endocrinology, 76, pp.80~89 (1965)
- Damiola F, "Restricted feeding uncouples circadian oscillators in peripheral tissues from the central pacemaker in the suprachiasmatic nucleus", Genes Dev, 14, pp.2950~2961 (2000)
- Assis MA, "Food intake and circadian rhythms in shift workers with a high workload", Appetite, 40, pp.175~183 (2003)
- El-Helaly M, "Workplace exposures and male infertility - a case-control study", Int J Occup Med Environ Health, 23, pp.331~338 (2010)
- Escobar C, "Persistence of metabolic rhythmicity during fastingand its entrainment by restricted feeding schedules inrats", Am J Physiol Regul Integr Comp Physiol, 274, pp.1309~1316 (1998)
- Ford ES, "Prevalence of metabolic syndrome among US adults: Findings from the third National Health and Nutrition Examination Survey", JAMA, 287, pp.356~359 (2002)
- Glass AR, "Fertility onset, spermatogenesis, and pubertal development in male rats: Effect of graded underfeeding", Pedriatr Res, 20, pp.1161~1167 (1986)
- Gooley JJ, "The dorsomedial hypothalamic nucleus is critical for the expression of food-entrainable circadian rhythms", Nat Neurosci, 9, pp.398~407 (2006)
- Grewall T, "Androgen secretion and spermatogenesis in rats following semistarvation", Proc Soc Exp Biol Med, 138, pp.723~727 (1971)
- Howland BE, "The influence of feed restriction and subsequent re-feeding on gonadotropin secretion and serum testosterone levels in male rats", J Reprod Fertil, 44, pp.429~436 (1975)
- Karlsson B, "Is there an association between shift work and having a metabolic syndrome? Results from a population based study of 27,485 people", Occup Environ Med, 58, pp.747~752 (2001)
- Byung Kook Kwak, "Effect of Feeding Time Shift on the Reproductive System in Male Rats", 발생과 생식, 16(1), pp.53~58 (2012), 한국발생생물학회
- Lawson CC, "Rotating shift work and menstrual cycle characteristics", Epidemiology, 22, pp.305~312 (2011)
- Lennernas M, "Shift related dietary intake in day and shift workers", Appetite, 25, pp.253~265 (1995)
- Levin S, "Effects of two weeks of feed restriction on some common toxicologic parameters in Sprague-Dawley rats", Toxicol Pathol, 21, pp.1~14 (1993)
- Michalakis K, "The complex interaction between obesity, metabolic syndrome and reproductive axis: A narrative review", Metabolism (2012)[DOI]
- Mühlbauer E, "Indication of circadian oscillations in the rat pancreas", FEBS Lett, 564, pp.91~96 (2004)
- Peschke E, "Evidence for a circadian rhythm of insulin release from perifused rat pancreatic islets", Diabetologia, 41, pp.1085~1092 (1998)
- Rehm S, "Effects of food restriction on testis and accessory sex glands in maturing rats", Toxicol Pathol, 36, pp.687~694 (2008)
- Rüdiger HW, "Health problems due to night shift work and jetlag", Internist, 45, pp.1021~1025 (2004)
- Santos AM, "Effects of undernutrition on serum and testicular testosterone levels and sexual function in adult rats", Horm Metabol Res, 36, pp.27~33 (2004)
- Teerds KJ, "Functional relationship between obesity and male reproduction: from humans to animal models", Hum Reprod Update, 17, pp.667~683 (2011)
- Wang XS, "Shift work and chronic disease: The epidemiologicalevidence", Occup Med, 61, pp.78~89 (2011)