본문 바로가기
KERD
논문KCI 등재

서울동물원 야생동물의 임상 검체 내 Clostridium 균의 항생제 내성 분석

Analysis of the anti-microbial susceptibility of Clostridium isolated on clinical specimens from captive wild animals in Seoul Zoo

김종택|안상진|이하늬|여용구
한국동물위생학회 (THE KOREAN SOCIETY OF VETERINARY SERVICE)|2020.03|한국동물위생학회지|제43권 제1호|pp. 31-37|DOI 10.7853/kjvs.2020.43.1.31

1

조회

0

다운로드

1

인용

0

참고문헌

키워드

Antibiotic resistanceZooAntibiotic susceptibilityWild animalClostridium
주제분류수의학

초록

Clostridial bacteria are zoonotic agents, which cause severe necrotizing enteritis, pseudo-membrane col-itis, enterotoxemia to both humans and animals. The objective of this study was to monitor the antibiotic resistance of Clostridium isolates on clinical specimens from wild animals in Seoul zoo for 5 years. Clostridium isolates were verified by using Vitek2 compact machine. Antibiotic susceptibility was as-sessed by antibiotic disc diffusion test, which was followed by Kirby-Bauer disc diffusion test method. The frequency of Antimicrobial resistance of Clostridium isolate was the greatest in gentamicin (87%), then in order of amikacin (80%). There were 55.6% of Clostridium isolates showed multiple drug resist-ance (MDR). These results showed that a lot of Clostridial bacteria from wild animals in Seoul zoo were acquired antibiotic resistance. Because of the wild animal’s aggressive manner, it has been hard to collect clinical samples from wild animals in a zoo to exam antibiotic susceptibility. For these reasons, empirical use of antibiotics has been performed in frequently. It may cause to increase the emergence of antibiotic resistance bacteria. In addition, the antibiotic resistance bacteria from zoo animals can be spread to other wild animals which inhabit around the zoo. Therefore, regular monitoring of antibiotic resistance Clostridial bacteria is important to protect animals and humans from Clostridial diseases.

Abstract

Clostridial bacteria are zoonotic agents, which cause severe necrotizing enteritis, pseudo-membrane col-itis, enterotoxemia to both humans and animals. The objective of this study was to monitor the antibiotic resistance of Clostridium isolates on clinical specimens from wild animals in Seoul zoo for 5 years. Clostridium isolates were verified by using Vitek2 compact machine. Antibiotic susceptibility was as-sessed by antibiotic disc diffusion test, which was followed by Kirby-Bauer disc diffusion test method. The frequency of Antimicrobial resistance of Clostridium isolate was the greatest in gentamicin (87%), then in order of amikacin (80%). There were 55.6% of Clostridium isolates showed multiple drug resist-ance (MDR). These results showed that a lot of Clostridial bacteria from wild animals in Seoul zoo were acquired antibiotic resistance. Because of the wild animal’s aggressive manner, it has been hard to collect clinical samples from wild animals in a zoo to exam antibiotic susceptibility. For these reasons, empirical use of antibiotics has been performed in frequently. It may cause to increase the emergence of antibiotic resistance bacteria. In addition, the antibiotic resistance bacteria from zoo animals can be spread to other wild animals which inhabit around the zoo. Therefore, regular monitoring of antibiotic resistance Clostridial bacteria is important to protect animals and humans from Clostridial diseases.

참고문헌 (21)

  1. Albertini MT, "Surveillance of methicillin-resistant Staphylococcus aureus(MRSA)and Enterobacteriaceae producing extended-spectrum beta-lactamase(ESBLE)in Northern France : a five-year multicentre incidence study", J Hosp Infect, 52(2), pp.107~113 (2002)
  2. Alekshun MN, "Targeting virulence to prevent infection : to kill or not to kill? Drug Discov", Today Ther Strateg, 1, pp.483~489 (2004)
  3. Alexander CJ, "Identification and antimicrobial resistance patterns of clinical isolates of Clostridium clostridioforme, Clostridium innocuum and Clostridium ramosen compared with these of clinical isolates of Clostridium perfringens", J Clin Microbiol, 33(12), pp.3209~3215 (1995)
  4. British Society for Antimicrobial Chemotherapy(BSAC), "Methods for antimicrobial susceptibility testing, Version 10.2", BSAC, ~ (2011)
  5. Bulger RJ, "Decreased incidence of antibiotic resistance among Staphylococcus aureus : a study in a university hospital over a 9-year period", Annals of internal medicine, 69(6), pp.1099~1108 (1968)
  6. Casadevall A, "Crisis in infectious diseases: time for a new paradigm?", Clin. Infect. Dis., 23(4), pp.790~794 (1996)
  7. Choi DY, "Frequency and antimicrobial susceptibility of bacterial isolates from domestic dogs", Master, pp.2006
  8. Esaki H, "Epidemiological Characterization of Salmonella Typhimurium DT 104. Prevalent among Food-Producing Animals in the Japanese Veterinary Antimicrobial Resistance Monitoring Program(1999-2001)", Microbiol Immunol, 48(7), pp.553~556 (2004)
  9. Center for drug evaluation and research, "FDA", Antimicrobial Resistance, ~ (2003)
  10. Harvey RA, "Lippincott’s Illustrated Reviews: Pharmacology", pp.389~404 (2012), Lippincott
  11. 장혜진, "Comparison of Harboring the Resistance Gene and Disc Diffusion Susceptibility Test Result in Staphylococcus pseudintermedius from the Bacterial Dermatitis", 한국임상수의학회지, 32(2), pp.158~161 (2015), 한국임상수의학회[DOI]
  12. Kau AL, "Human nutrition, the gut microbiome and the immune system", Nature, 474(7351), pp.327~336 (2011)
  13. 김효식, "Clostridium difficile 아포에 대한 소독제의 살균효과", 의료관련감염관리, 16(1), pp.13~17 (2011), 대한의료관련감염관리학회
  14. KIM, JEONG-DONG, "Distribution and Antimicrobial Susceptibility of Clostridium Species in SoilContaminated with Domestic Livestock Feces of Korea", Journal of Microbiology and Biotechnology, 14(2), pp.401~410 (2004), 한국미생물·생명공학회
  15. King A, "Quality assurance of antimicrobial susceptibility testing by disc diffusion", J Antimicrob Chemother, 48, pp.71~76 (2001)
  16. Murthy R, "Implementation of strategies to control antimicrobial resistance", Chest, 119, pp.405S~411S (2001)
  17. Ozawa Y, "Vancomycin-resistant enterococci in humans and imported chickens in Japan", Appl Environ Microbiol, 68, pp.6457~6461 (2002)
  18. Phillips I, "Does the use of antibiotics in food animals pose a risk to human health? A critical review of published data", J Antimicrob Chemother, 53(1), pp.28~52 (2004)
  19. Plumb DC, "Veterinary drug handbook", pp.447~449 (2002), Iowa State Press
  20. Song JH, "Unique alteration in Penicillin-binding protein 2B of multidrug-resistant Streptococcus pneumoniae from Korea", Kor J Infect Dis, 32, pp.108~114 (2000)
  21. 성명숙, "가금 유래 병원성대장균의 생화학적 성상 및 혈청형", 대한수의학회지, 48(2), pp.145~151 (2008), 대한수의학회

관련 논문AI추천

경남 북부지역 오리 분변에서 분리된 Campylobacter spp.의 항생제 내성고필옥, 김형수, 박동엽, 박동주, 박정용, 서덕진, 성민호, 정명호, 한권식한국동물위생학회 · 한국동물위생학회지2017 · 제40권 제2호시판 젓갈에서 분리한 Clostridium perfringens의 독소 유전자 및 항균제 내성 분석박권삼, 이신혜한국수산과학회 · 한국수산과학회지2023 · 제56권 제6호서울지역의 개와 고양이에서 분리한 Staphylococcus aureus의 항생제 감수성 및 multiplex PCR을 이용한 장독소의 검출김능희, 김선흥, 김창기, 김철훈, 손홍락, 이정학, 채희선한국동물위생학회 · 한국동물위생학회지2010 · 제33권 제3호Multidrug resistance of coagulase-negative staphylococci isolated from rescued wild animalsHaerin Rhim, Hong-Cheul Kim, Jae-Ik Han, Ki-Jeong Na한국동물위생학회 · 한국동물위생학회지2019 · 제42권 제4호개와 고양이에서 분리한 Pseudomonas aeruginosa의 항생제 내성Chang-Ryoul Yang, Hyun-Suk Lim, Jae-Keun Cho, Jeong-Mi Kim, Kyung-Hee Kim한국동물위생학회 · 한국동물위생학회지2021 · 제44권 제1호Investigation of Toxin Gene Diversity, Molecular Epidemiology, and Antimicrobial Resistance of Clostridium difficile Isolated from 12 Hospitals in South Korea어영, 김미나, 김종완, 김희정, 노경호, 신명근, 신희봉, 이경원, 이혁민, 정석훈, 홍성근대한진단검사의학회 · Annals of Laboratory Medicine2010 · 제30권 제5호

이 간행물의 다른 논문 (5건)

인천지역 공원 채집 모기에서 심장사상충 감염도 조사김경호, 정철, 송재성, 이성모, 김명덕, 이정구pp. 1-6개똥쑥의 첨가 급이가 육계의 혈액 성분 및 계육의 지방산 조성에 미치는 영향이수정, 조항희, 조재현pp. 7-16전국에서 도축된 가축의 잔류검사에서 검출된 잔류물질 비교조사박찬일, 방성민, 고대성, 김지향, 박양순pp. 17-21광주지역 도축 돼지 및 가공품 E형 간염 실태 조사박자윤, 김용환, 박지영, 김지연, 곽진주, 장미선, 최인수, 정하진, 성창민, 안아진, 서계원pp. 23-29Exposure to low concentrations of mycotoxins triggers unique responses from the pig gut microbiomeHo-Seong Cho, 오연수, Sang-Eog Koh, Sung-Hyun Moonpp. 39-44
원문 보기