본문 바로가기
KERD
논문KCI 등재

저강도 고반복 전기자극이 노화된 단일 근섬유의 위성세포 활성 및 단백질 합성 신호체계에 미치는 영향

The effects of low-frequncy high-volume electrical stimulation on satellite cell activation and anabolic signaling pathway in single muscle fibers of old mice

김효정|김창근|임창현|Sophie Joanisse|Stuart M. Phillips
국민체육진흥공단 한국스포츠과학원 (Korea Institute of Sport Science)|2020.12|체육과학연구 (Korean Journal of Sport Science)|제31권 제4호|pp. 638-649|DOI 10.24985/kjss.2020.31.4.638

1

조회

0

다운로드

1

인용

0

참고문헌

키워드

Skeletal muscle골격근Protein synthesis저강도 고반복전기자극단백질 합성Low-load high-volumeElectrical pulse stimulation
주제분류체육

초록

[목적] 이 연구는 저강도 고반복 저항성 근수축이 노화된 단일 근섬유의 위성세포 활성, mechanistic target of rapamycin (mTOR), mitogen-activated protein kinase (MAPK) 신호체계에 미치는 영향을 확인하였다. [방법] 12주령(젊은 집단, n=21)과 72주령(노화 집단, n=21)의 C57BL/6 마우스로부터 단일 근섬유를 분리 및 배양한 후 근수축 형태에 따른 영향을 분석하기 위하여 1) 전기자극을 시행하지 않은 통제집단(Con), 2) 저강도 저반복 전기자극 집단(LL), 3) 저강도 고반복 전기자극 집단(LH), 4) 고강도 저반복 전기자극 집단(HL)으로 무작위 배정하였다. 전기자극이 시행된 단일 근섬유는 조직면역화학염색을 이용하여 위성세포의 활성을 분석하였으며, western-blotting을 실시하여 단백질 합성 신호체계의 인산화를 분석하였다. [결과] Quiescent 위성세포(Pax7+Ki67-), 활성 위성세포(Pax7+Ki67+), 근 핵의 숫자와 4E-BP1, ERK의 인산화 정도는 노화 집단이 젊은 집단에 비해 유의하게 낮았다(p<.05). 그러나 연령에 상관없이 LH와 HL 전기자극은 Con 집단보다 위성세포의 활성(142%, both)과 mTOR (129% and 133%, respectively)와 p70S6K (133% and 136%, respectively), 4E-BP1 (140% and 129%, respectively)의 인산화를 유의하게 증가시켰다(p<.05). ERK의 인산화는 젊은 집단과 노화 집단 모두 오직 LH 전기자극에 의해서만 증가 되었다(123% and 125%, respectively). [결론] 본 연구의 결과는 저강도 고반복 전기자극이 노화된 단일 근섬유의 위성세포 활성과 mTOR 단백질 신호체계의 인산화를 젊은 집단과 유사하게 증가시킬 수 있음을 확인하였다.

Abstract

This is the first study to examine whether age impacts the response of single muscle fibers to high/low frequency and high/low volume electrical pulse stimulation. We performed in vitro experiments to evaluate the effect of low-frequency high-volume electrical pulse stimulation (EPS) on mechanistic target of rapamycin (mTOR), mitogen-activated protein kinase (MAPK) signaling pathway, and satellite cell activation in singles fibers of young and aged muscles. [Methods] Isolated single fibers from gastocnemius in 12-wk (n=21) and 72-wk (n=21) old male C57BL/6 mice were divided into four groups: 1) control (Con) received no EPS, 2) low-frequency low-volume EPS (LL), 3) low-frequency high-volume EPS (LH), and 4) high-frequency low-volume EPS (HL) were made to contract using independent EPS protocols. Satellite cell activation and anabolic pathway (mTOR and MAPK signaling) were measured before and after EPS. [Results] The number of quiescent (Pax7+/Ki67-) and active (Pax7+/Ki67+) satellite cells, myonuclear content and the phosphorylation of 4E-BP1 and ERK were higher in young when compared with old. However, regardless of age, LH and HL EPS significantly increased the number of activate satellite cells (142%, both) and phosphorylation of mTOR (129% and 133%, respectively), p70S6K (133% and 136%, respectively) and 4E-BP1 (140% and 129%, respectively) compared with Con. The protein expression of ERK phosphorylation only increased by LH EPS in both the young and old groups (123% and 125%, respectively). [Conclusion] Low-frequency high-volume EPS stimulated satellite cell activation and the mTOR signaling pathway in older similar to young muscle.

참고문헌 (41)

  1. American College of Sports Medicine, "American College of Sports Medicine position stand. Exercise and physical activity for older adults", Med Sci Sports Exerc, 41(7), pp.1510~1530 (2009)
  2. Burd, N. A., "Low-load high volume resistance exercise stimulates muscle protein synthesis more than high-load low volume resistance exercise in young men", PLoS One, 5(8), pp.e12033~ (2010)
  3. Burnette, W. N., "“Western blotting”: electrophoretic transfer of proteins from sodium dodecyl sulfate--polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A", Anal Biochem, 112(2), pp.195~203 (1981)
  4. Burton L. A., "Optimal management of sarcopenia", Clin Interv Aging, 7(5), pp.217~228 (2010)
  5. Carter, S., "In vitro experimental models for examining the skeletal muscle cell biology of exercise: the possibilities, challenges and future developments", Pflugers Arch, 471(3), pp.413~429 (2019)
  6. Donnelly, K., "A novel bioreactor for stimulating skeletal muscle in vitro", Tissue Eng Part C Methods, 16(4), pp.711~718 (2010)
  7. Hennig, R., "Firing patterns of motor units in normal rats", Nature, 314(6007), pp.164~166 (1985)
  8. Hornberger, T. A., "Mechanical stimuli regulate rapamycin-sensitive signalling by a phosphoinositide 3-kinase-, protein kinase B- and growth factor-independent mechanism", Biochem J, 380(Pt 3), pp.795~804 (2004)
  9. Joanisse S., "Skeletal Muscle Regeneration, Repair and Remodelling in Aging: The Importance of Muscle Stem Cells and Vascularization", Gerontology, 63(1), pp.91~100 (2017)
  10. Joanisse, S., "Recent advances in understanding resistance exercise training-induced skeletal muscle hypertrophy in humans", F1000Res, 9, pp.141~ (2020)
  11. Kirwan J. P., "Human aging is associated with altered TNF-alpha production during hyperglycemia and hyperinsulinemia", Am J Physiol Endocrinol Metab, 281(6), pp.E1137~E1143 (2001)
  12. Kumar, V., "Age-related differences in the dose-response relationship of muscle protein synthesis to resistance exercise in young and old men", J Physio, 587(1), pp.211~217 (2009)
  13. Lambernd, S., "Contractile activity of human skeletal muscle cells prevents insulin resistance by inhibiting pro-inflammatory signalling pathways", Diabetologia, 55(4), pp.1128~1139 (2012)
  14. Laplante. M., "mTOR signaling in growth control and disease", Cell, 149(2), pp.274~293 (2012)
  15. 임창현, "일회성 저강도 고반복 저항성 운동이 골격근 합성신호 단백질 발현 및 위성세포 활성에 미치는 영향", 운동과학, 29(1), pp.77~85 (2020), 한국운동생리학회
  16. Lim, C., "Resistance Exercise-induced Changes in Muscle Phenotype Are Load Dependent", Med Sci Sports Exerc, 51(12), pp.2578~2585 (2019)
  17. Liu, Y., "Activity-dependent nuclear translocation and intranuclear distribution of NFATc in adult skeletal muscle fibers", J Cell Biol, 155(1), pp.27~39 (2001)
  18. Lluís, F., "Regulation of skeletal muscle gene expression by p38 MAP kinases", Trends Cell Biol, 16(1), pp.36~44 (2006)
  19. Mackey A. L., "Assessment of satellite cell number and activity status in human skeletal muscle biopsies", Muscle Nerve, 40(3), pp.455~465 (2009)
  20. Mitchell, C. J., "Resistance exercise load does not determine training-mediated hypertrophic gains in young men", J Appl Physiol, 113(1), pp.71~77 (2012)
  21. Naumann, K., "Effects of chronic stimulation with different impulse patterns on the expression of myosin isoforms in rat myotube cultures", Differentiation, 55(3), pp.203~211 (1994)
  22. Nikolić, N., "Electrical pulse stimulation of cultured human skeletal muscle cells as an in vitro model of exercise", PLoS One, 7(3), pp.e33203~ (2012)
  23. Nikolić, N., "Electrical pulse stimulation of cultured skeletal muscle cells as a model for in vitro exercise - possibilities and limitations", Acta Physiol (Oxf), 220(3), pp.310~331 (2017)
  24. O'Neil, T. K., "The role of phosphoinositide 3-kinase and phosphatidic acid in the regulation of mammalian target of rapamycin following eccentric contractions", J Physiol, 587(Pt 14), pp.3691~3701 (2009)
  25. Ogasawara, R., "The role of mTOR signalling in the regulation of skeletal muscle mass in a rodent model of resistance exercise", Sci Rep, 9(6), pp.31142~ (2016)
  26. Osman, A. A., "Regulation of MAP kinase pathway activity in vivo in human skeletal muscle", Am J Physiol Endocrinol Metab, 278(6), pp.E992~E999 (2000)
  27. Paolisso G., "Advancing age and insulin resistance: role of plasma tumor necrosis factor-alpha", Am JPhysiol, 275(2), pp.E294~E299 (1998)
  28. Park D. R., "Exercise ameliorates insulin resistance via Ca2+ signals distinct from those of insulin for GLUT4 translocation in skeletal muscles", Diabetes, 64(4), pp.1224~1234 (2015)
  29. Raingeaud J., "Pro-inflammatory cytokines and environmental stress cause p38 mitogen-activated protein kinase activation by dual phosphorylation on tyrosine and threonine", J Biol Chem, 270(13), pp.7420~7426 (1995)
  30. Rosenblatt J. D., "Culturing satellite cells from living single muscle fiber explants", In Vitro Cell Dev Biol Anim, 31(10), pp.773~779 (1995)
  31. Rosenberg, I. H., "Sarcopenia: origins and clinical relevance", J Nutr, 127(5 Suppl), pp.990S~991S (1997)
  32. Scheler, M., "Cytokine response of primary human myotubes in an in vitro exercise model", Am J Physiol Cell Physiol, 305(8), pp.C877~C886 (2013)
  33. Schoenfeld, B. J., "Strength and Hypertrophy Adaptations Between Low- vs. High-Load Resistance Training: A Systematic Review and Meta-analysis", J Strength Cond Res, 31(12), pp.3508~3523 (2017)
  34. Sengupta, S., "Regulation of the mTOR complex 1 pathway by nutrients, growth factors, and stress", Mol Cell, 40(2), pp.310~322 (2010)
  35. Spangenburg, E. E., "A functional insulin-like growth factor receptor is not necessary for load-induced skeletal muscle hypertrophy", J Physiol, 586(1), pp.283~291 (2008)
  36. Stump C. S., "The metabolic syndrome: role of skeletal muscle metabolism", Ann Med, 38(6), pp.389~402 (2006)
  37. Suetta C., "Ageing is associated with diminished muscle re-growth and myogenic precursor cell expansion early after immobility-induced atrophy in human skeletal muscle", J Physiol, 591(15), pp.3789~3804 (2013)
  38. Vandenburgh, H. H., "Insulin and IGF-I induce pronounced hypertrophy of skeletal myofibers in tissue culture", Am J Physiol, 260(3 Pt 1), pp.C475~C484 (1991)
  39. Verdijk, L. B., "Satellite cells in human skeletal muscle; from birth to old age", Age (Dordr), 36(2), pp.545~547 (2014)
  40. Williamson, D., "Mitogen-activated protein kinase (MAPK) pathway activation: effects of age and acute exercise on human skeletal muscle", J Physiol, 547(Pt 3), pp.977~987 (2003)
  41. Yang, Y., "Resistance exercise enhances myofibrillar protein synthesis with graded intakes of whey protein in older men", Br J Nutr, 108(10), pp.1780~1788 (2012)

관련 논문AI추천

일회성 저강도 고반복 저항성 운동이 골격근 합성신호 단백질 발현 및 위성세포 활성에 미치는 영향김창근, 임창현한국운동생리학회 · 운동과학2020 · 제29권 제1호Effects of Resistance Exercise Training on Aged Skeletal Muscle: Potential Role of Muscle Stem Cells강년주, 김동일, 박영민한국운동생리학회 · 운동과학2023 · 제32권 제2호전기자극 조건에 따른 근육 세포에 미치는 영향과 반응김민석, 서형우, 신현영, 이현주, 태기식대한의용생체공학회 · 의공학회지2017 · 제38권 제6호저빈도 전기자극이 뒷다리 무부하 상태의 흰쥐 골격근 IGF-1 발현에 미치는 영향강종호특수교육재활과학연구소 · 특수교육재활과학연구2009 · 제48권 제4호6주 분할운동이 노화된 흰쥐 골격근 내 자식작용과 근육형성조절인자의 발현에 미치는 영향김정석한국운동생리학회 · 운동과학2014 · 제23권 제1호저산소 환경의 지구성 운동이 근육 위성세포의 활성 및 증식에 미치는 효과손희정, 임창현한국운동생리학회 · 운동과학2019 · 제28권 제1호

이 간행물의 다른 논문 (18건)

운동과 암: 예방에서 치료까지?서한교, 곽이섭pp. 603-611트레드밀 운동이 알츠하이머 질환의 미토콘드리아 항상성 기전에 미치는 영향권기천, 엄현섭, 조준용, 오은택, 최동훈pp. 612-625저온환경에의 급성노출이 에너지 대사, 골격근 산소화 농도 및 운동수행능력에 미치는 영향서종범, 임기원, 박훈영, 황든솔, 견성환, 김지수, 박예람, 김성우, 정경화, 장인권, 황혜정, 하유진, 정원상pp. 626-637축구 선수의 소셜 미디어 내용 분석을 위한 온톨로지 모형 연구김주학, 조선미, 강지연pp. 650-661엘리트 청소년 축구선수에 대한 Rosenberg 자아존중감 척도의 문항 및 반응범주 기능검사: Rasch 모형 활용강민수, 김헌태, 정명진pp. 662-671
원문 보기